本文選題：康復(fù)訓(xùn)練　切入點：血管性癡呆　出處：《南華大學(xué)》2013年碩士論文

【摘要】：目的：研究康復(fù)訓(xùn)練調(diào)控海馬β淀粉樣蛋白改善血管性癡呆大鼠模型認知功能的機制。 方法：將45只大鼠用隨機數(shù)字表法分為模型組、康復(fù)組和假手術(shù)組。采用雙側(cè)頸總動脈結(jié)扎法制作血管性癡呆大鼠模型�？祻�(fù)組大鼠行滾筒、平衡木訓(xùn)練4周。術(shù)后4周進行自發(fā)活動實驗、水迷宮、Y迷宮、新舊物體識別實驗進行大鼠行為學(xué)評估。采用大鼠海馬切片HE染色觀察海馬形態(tài)改變,并行免疫組化染色檢測海馬β淀粉樣蛋白、β分泌酶、胰島素降解酶表達情況。 結(jié)果：1.自發(fā)活動檢測結(jié)果:模型組、康復(fù)組、假手術(shù)組大鼠總活動時間、中央?yún)^(qū)活動時間、外周活動時間、水平活動距離、中央活動距離和周圍活動距離差異均無顯著性(p0.05)。 2. Y迷宮實驗:模型組Y迷宮實驗正確交替比率低于假手術(shù)組(p0.05)、康復(fù)組Y迷宮實驗正確交替率高于模型組(p0.05)，康復(fù)組Y迷宮實驗正確交替比率低于假手術(shù)組(p0.05)。 3.水迷宮實驗:模型組水迷宮穿過平臺次數(shù)低于假手術(shù)組、水迷宮實驗潛伏期長于假手術(shù)組(p0.05)�？祻�(fù)組水迷宮穿過平臺次數(shù)高于模型組、水迷宮實驗潛伏期長于模型組(p0.05)。康復(fù)組水迷宮穿過平臺次數(shù)低于假手術(shù)組、水迷宮實驗潛伏期長于假手術(shù)組(p0.05)。 4.新舊物體識別實驗:模型組新舊物體識別實驗分辨指數(shù)低于假手術(shù)組(p0.05)。模型組新舊物體識別實驗分辨指數(shù)低于康復(fù)組(p0.05)�？祻�(fù)組新舊物體識別實驗分辨指數(shù)低于假手術(shù)組(p0.05)。 5. HE染色:低倍鏡下模型組大鼠海馬DG區(qū)細胞數(shù)目少,排列稀疏,康復(fù)組大鼠海馬DG區(qū)細胞數(shù)較假手組大鼠稀疏但較模型組多。高倍鏡下,模型組海馬DG區(qū)空泡細胞多,康復(fù)組亦可見少量空泡細胞,假手術(shù)組未見空泡細胞。 6. β-淀粉樣蛋白免疫組化表達結(jié)果:模型組海馬β淀粉樣蛋白表達高于假手術(shù)組(p0.05)�？祻�(fù)組海馬β淀粉樣蛋白表達低于模型組(p0.05)。康復(fù)組海馬β淀粉樣蛋白表達高于假手術(shù)組(p0.05)。 7.各組大鼠海馬β分泌酶免疫組化表達結(jié)果:模型組海馬β分泌酶表達高于假手術(shù)組(p0.05)�？祻�(fù)組海馬β分泌酶表達低于模型組(p0.05)。康復(fù)組海馬β分泌酶表達高于假手術(shù)組(p0.05)�？祻�(fù)組海馬β分泌酶表達高于假手術(shù)組(p0.05)。 8.各組大鼠海馬胰島素降解酶免疫組化表達結(jié)果:模型組海馬胰島素降解酶表達低于假手術(shù)組(p0.05)�？祻�(fù)組海馬胰島素降解酶表達高于模型組(p0.05)�？祻�(fù)組海馬胰島素降解酶表達低于假手術(shù)組(p0.05)。結(jié)論：康復(fù)訓(xùn)練可改善血管性癡呆大鼠認知功能，，這種保護作用可能通過下調(diào)BACE及上調(diào)IDE在VD大鼠海馬DG區(qū)的表達來減少Aβ在海馬DG區(qū)的表達。
[Abstract]:Aim: to study the mechanism of rehabilitation training on the improvement of cognitive function of vascular dementia rats by 尾 -amyloid in hippocampus.Methods: 45 rats were randomly divided into three groups: model group, rehabilitation group and sham operation group.Vascular dementia rat model was established by bilateral common carotid artery ligation.Rats in rehabilitation group were trained with roller and balance beam for 4 weeks.Spontaneous activity test, water maze and Y maze were performed 4 weeks after operation.The changes of hippocampal morphology were observed by HE staining and the expression of 尾 -amyloid, 尾 -secretase and insulin-degrading enzyme in hippocampus were detected by immunohistochemical staining.The result is 1: 1.Results of spontaneous activity detection: there was no significant difference in total activity time, central area activity time, peripheral activity time, horizontal activity distance, central activity distance and peripheral activity distance between model group, rehabilitation group and sham operation group.2.Y maze experiment: the correct alternating rate of Y maze test in model group was lower than that in sham operation group, the correct alternating rate of Y maze test in rehabilitation group was higher than that in model group, and the correct alternation rate of Y maze experiment in rehabilitation group was lower than that in sham operation group.3.Water maze test: the number of water labyrinth passing through the platform in the model group was lower than that in the sham operation group, and the incubation period of the water maze test was longer than that of the sham operation group.The times of water labyrinth passing through the platform in rehabilitation group were higher than that in model group, and the latency of water maze test was longer than that of model group (P 0.05).The times of water labyrinth passing through the platform in rehabilitation group were lower than that in sham operation group, and the latency of water maze test was longer than that of sham operation group.4.New and old object recognition experiment: the resolution index of model group was lower than that of sham-operation group (P 0.05).The experimental resolution index of old and new object recognition in model group was lower than that in rehabilitation group (P 0.05).The experimental resolution index of old and new object recognition in rehabilitation group was lower than that in sham operation group (P 0.05).5.He staining: the number of DG cells in the hippocampus of the model group was less and the number of the cells in the hippocampal DG region of the rehabilitation group was less than that of the artificial hand group but more than that of the model group.Under high power microscope, there were more vacuoles in DG area of hippocampus in model group, a small number of vacuoles in rehabilitation group, and no vacuole cells in sham operation group.6. Expression of 尾 -amyloid protein: the expression of 尾 -amyloid protein in hippocampus of model group was higher than that of sham operation group (P 0.05).The expression of 尾 -amyloid protein in hippocampus of rehabilitation group was lower than that of model group.The expression of 尾 -amyloid protein in hippocampus of rehabilitation group was higher than that of sham operation group.7.The expression of 尾 -secretase in hippocampus of model group was higher than that of sham operation group (P 0.05).The expression of 尾 -secretase in hippocampus of rehabilitation group was lower than that of model group.The expression of 尾 -secretase in hippocampus of rehabilitation group was higher than that of sham operation group (P 0.05).The expression of 尾 -secretase in hippocampus of rehabilitation group was higher than that of sham operation group (P 0.05).8.The expression of insulinase in hippocampus of the model group was lower than that of the sham-operated group (P 0.05).The expression of insulin-degrading enzyme in hippocampus of rehabilitation group was higher than that of model group.The expression of insulinase in hippocampus of rehabilitation group was lower than that of sham operation group (P 0.05).Conclusion: rehabilitation training can improve the cognitive function of vascular dementia rats. This protective effect may reduce the expression of A 尾 in the DG region of hippocampus by down-regulating BACE and up-regulating the expression of IDE in the DG region of hippocampus of VD rats.
【學(xué)位授予單位】：南華大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R749.13

【共引文獻】

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