本文選題：精神分裂癥　切入點(diǎn)：抑郁癥　出處：《解放軍醫(yī)學(xué)雜志》2017年04期 　論文類型：期刊論文

【摘要】：目的觀察精神分裂癥(SZ)患者中差異表達(dá)的microRNA(miRNA),從miRNA表達(dá)水平上分析SZ與抑郁癥的關(guān)系。方法通過(guò)基因芯片篩選在SZ患者中差異表達(dá)的miRNA。采用實(shí)時(shí)定量PCR(qRT-PCR)在40例SZ患者外周血單核細(xì)胞中驗(yàn)證芯片篩查結(jié)果,并進(jìn)行精神分裂癥陽(yáng)性和陰性癥狀量表(PANSS)評(píng)定,同時(shí)檢測(cè)抑郁癥中差異表達(dá)的5種miRNA(miR-1972、miR-26b、miR-4485、miR-4498、miR-4743)的表達(dá)改變及其與SZ中差異表達(dá)的miRNA及PANSS評(píng)分的相關(guān)性。結(jié)果與正常人相比,SZ患者中33種miRNA存在差異表達(dá)(32種表達(dá)上調(diào),1種表達(dá)下調(diào)),且其中8種上調(diào)的miRNA(miR-1273d、miR-1303、miR-3064-5p、miR-3131、miR-3687、miR-4428、miR-4725-3p、miR-5096)表達(dá)差異有統(tǒng)計(jì)學(xué)意義(P0.05)。在抑郁癥中差異表達(dá)的5種miRNA在SZ患者中差異表達(dá)也有統(tǒng)計(jì)學(xué)意義(P0.05),且與SZ中差異表達(dá)的8種miRNA呈中、高度相關(guān)(r=0.607~0.909,P0.01),其中miR-1972與PANSS量表陽(yáng)性癥狀得分呈顯著正相關(guān)(r=0.339,P0.05),miR-26b與復(fù)合量表因子分呈顯著正相關(guān)(r=0.342,P0.05)。結(jié)論 SZ與抑郁癥不僅具有某些共同的臨床表現(xiàn),二者在分子遺傳學(xué)方面也可能有共同的病理基礎(chǔ)。
[Abstract]:Objective to observe the differential expression of miRNAs in patients with schizophrenia (SZ) and analyze the relationship between SZ and depression from the level of miRNA expression. Methods the differentially expressed miRNAs in patients with SZ were screened by gene microarray. Real-time quantitative PCR qRT-PCR was used in 40 patients with SZ. The results of microarray screening in peripheral blood monocytes, The positive and negative symptoms of schizophrenia were assessed by PANSS. At the same time, the expression of 5 miRNAs differentially expressed miR-1972miR-26bsimiR-4485miR-4498 miR-4743) and their correlation with miRNA and PANSS scores in SZ patients were detected. Results there were 32 differential expressions of 32 kinds of miRNA in SZ patients compared with the normal controls. Eight upregulated miRNAs miR-1303mmiR-3064-5pmiR-3131miR-3687miR-44687 miR-4725-3pmmiR-5096) were down-regulated, and there were significant differences in the expression of 5 kinds of miRNAs in patients with depression (P0.05A), and there was also a significant difference in the expression of five kinds of miRNA in patients with SZ, and there was a significant difference in the expression of P0.05G between the 5 kinds of miRNAs in patients with depression, and the difference of miR-3064-5pmiR-3131miR-4687 miR-4725-3pmmiR-5096 (P < 0.05). There was a significant positive correlation between the positive symptoms of miR-1972 and PANSS and the factor score of compound scale. Conclusion SZ and depression not only have some common clinical manifestations, but also have significant positive correlation with the score of compound scale. They may also share the same pathological basis in molecular genetics.
【作者單位】： 徐州醫(yī)科大學(xué)研究生學(xué)院;解放軍102醫(yī)院全軍心理疾病防治中心;解放軍102醫(yī)院心身科;解放軍102醫(yī)院神經(jīng)科;解放軍102醫(yī)院檢驗(yàn)科;GoPath
【基金】：南京軍區(qū)醫(yī)學(xué)科技創(chuàng)新課題(12MA002)~~
【分類號(hào)】：R749.3

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