白細胞介素-6基因多態(tài)性與膿毒癥的相關性研究
發(fā)布時間：2019-02-23 13:47
【摘要】：目的：研究白細胞介素-6基因啟動子區(qū)rs1800796C/G的等位基因及其基因型在廣西地區(qū)人群中的分布頻率，探討IL-6rs1800796C/G基因多態(tài)性與膿毒癥的關系。 方法：采用單堿基延伸的PCR技術和DNA測序法，檢測199例廣西健康體檢人群和152例膿毒癥患者的IL-6基因啟動子區(qū)rs1800796C/G多態(tài)性，并分析其多態(tài)性與膿毒癥的相關性。 結果：廣西人群IL-6基因啟動子區(qū)rs1800796位點存在G、C兩種等位基因及CC、CG、GG三種單核苷酸多態(tài)基因型。其基因型及等位基因頻率在男女組間比較差異無統(tǒng)計學意義（P＞0.05），兩種等位基因的分布在膿毒癥患者與對照組間無顯著差異（P＞0.05）。與非洲人、歐洲人、日本人和北京人等四個人群的SNP分型數(shù)據(jù)進行比較，廣西人群的基因型及等位基因分布頻率與日本人和北京人比較差異無統(tǒng)計學意義（均P＞0.05），而與歐洲人、非洲人比較差異有統(tǒng)計學意義（均P＜0.05）。廣西人、北京人和日本人CC基因型所占比例較高，，而非洲人和歐洲人GG型頻率較高。膿毒癥患者血清IL-6水平明顯增高（P＜0.05），IL-6rs1800796C/G基因多態(tài)性與膿毒癥患者血清IL-6水平無相關性。 結論：廣西人群的IL-6基因啟動子區(qū)rs1800796C/G存在基因多態(tài)性，其基因型分布及等位基因分布頻率與性別無相關性，但與其他種族人群相比存在明顯差異。IL-6rs1800796C/G基因多態(tài)性與膿毒癥易感性和血清IL-6水平無相關性。
[Abstract]:Objective: to study the alleles of interleukin-6 (IL-6) gene promoter region rs1800796C/G and its genotype distribution in Guangxi population, and to explore the relationship between IL-6rs1800796C/G gene polymorphism and sepsis. Methods: single base extension PCR technique and DNA sequencing were used to detect the rs1800796C/G polymorphism of IL-6 gene promoter in 199 healthy controls and 152 sepsis patients, and the correlation between IL-6 gene promoter polymorphism and sepsis was analyzed. Results: there were two alleles in rs1800796 locus of IL-6 gene promoter and three single nucleotide polymorphism genotypes of CC,CG,GG in Guangxi population. There was no significant difference in genotype and allele frequency between male and female groups (P > 0.05), but there was no significant difference in the distribution of the two alleles between septic patients and controls (P > 0.05). Compared with the SNP genotyping data of African, European, Japanese and Beijingese, there was no significant difference in genotype and allele distribution between Guangxi population and Japanese population and Beijingese population (all P > 0. 05). The difference between African and European was statistically significant (all P < 0.05). The proportion of CC genotypes was higher in Guangxi, Beijing and Japan, but the frequency of GG was higher in Africans and Europeans. Serum IL-6 level was significantly higher in septic patients (P < 0. 05). There was no correlation between IL-6rs1800796C/G gene polymorphism and serum IL-6 level in septic patients. Conclusion: there is a polymorphism of rs1800796C/G in the promoter region of IL-6 gene in Guangxi population, and there is no correlation between genotype distribution and allele distribution. IL-6rs1800796C/G gene polymorphism was not associated with susceptibility to sepsis and serum IL-6 level.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R459.7
本文編號：2428892
[Abstract]:Objective: to study the alleles of interleukin-6 (IL-6) gene promoter region rs1800796C/G and its genotype distribution in Guangxi population, and to explore the relationship between IL-6rs1800796C/G gene polymorphism and sepsis. Methods: single base extension PCR technique and DNA sequencing were used to detect the rs1800796C/G polymorphism of IL-6 gene promoter in 199 healthy controls and 152 sepsis patients, and the correlation between IL-6 gene promoter polymorphism and sepsis was analyzed. Results: there were two alleles in rs1800796 locus of IL-6 gene promoter and three single nucleotide polymorphism genotypes of CC,CG,GG in Guangxi population. There was no significant difference in genotype and allele frequency between male and female groups (P > 0.05), but there was no significant difference in the distribution of the two alleles between septic patients and controls (P > 0.05). Compared with the SNP genotyping data of African, European, Japanese and Beijingese, there was no significant difference in genotype and allele distribution between Guangxi population and Japanese population and Beijingese population (all P > 0. 05). The difference between African and European was statistically significant (all P < 0.05). The proportion of CC genotypes was higher in Guangxi, Beijing and Japan, but the frequency of GG was higher in Africans and Europeans. Serum IL-6 level was significantly higher in septic patients (P < 0. 05). There was no correlation between IL-6rs1800796C/G gene polymorphism and serum IL-6 level in septic patients. Conclusion: there is a polymorphism of rs1800796C/G in the promoter region of IL-6 gene in Guangxi population, and there is no correlation between genotype distribution and allele distribution. IL-6rs1800796C/G gene polymorphism was not associated with susceptibility to sepsis and serum IL-6 level.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R459.7
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本文編號：2428892
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