【摘要】：目的：觀察不同干預(yù)所致低血壓時雙側(cè)頸動脈中度狹窄家兔模型血漿神經(jīng)元特異性烯醇化酶(neuron-specific enolase, NSE)及S-100β蛋白濃度的變化,探討圍術(shù)期低血壓對頸動脈狹窄兔腦組織的損傷作用。 方法：選擇成年家兔建立雙側(cè)頸動脈中度狹窄家兔模型,采用完全隨機(jī)法分為四組,除對照組外,其他三組分別采用放血,硝酸甘油,丙泊酚使血壓降低20%-30%并維持20分鐘后分別采用相對應(yīng)的方法恢復(fù)血壓,分別于恢復(fù)血壓后7.5、24h取血液標(biāo)本,離心取上清液,用酶聯(lián)免疫吸附測定法(ELISA法)測定血清中NSE、S-100β蛋白,將腦損傷的程度做比較。 結(jié)果：與對照組比較3個降壓組的血液標(biāo)本中NSE及S-100β在各個觀測時間點的值均升高,差別有統(tǒng)計學(xué)意義(P0.05)。丙泊酚組在降壓7.5h的NSE及S-100β的表達(dá)值分別為(1487±19)μg/L和(592±10)μg/L,與放血組、硝酸甘油組相比較各時間點NSE蛋白表達(dá)指標(biāo)的升高值較小,差異有統(tǒng)計學(xué)意義(P0.05),S-100β蛋白的表達(dá)7.5h較其余兩個實驗組低,差異有統(tǒng)計學(xué)意義(P0.05)。 結(jié)論：對于雙側(cè)頸動脈中度狹窄兔模型,放血、硝酸甘油及麻醉藥物引起的血壓降低20%-30%均引起NSE及S-100β蛋白的表達(dá)升高,其中丙泊酚組的升高程度相對較低,可能與丙泊酚的腦保護(hù)作用有關(guān)。
[Abstract]:Objective: to observe the changes of plasma neuron-specific enolase (neuron-specific enolase, NSE) and S-100 尾 protein levels in bilateral carotid artery stenosis model of rabbits induced by hypotension induced by different interventions. To investigate the effect of perioperative hypotension on brain tissue injury in carotid stenosis rabbits. Methods: the bilateral carotid artery stenosis model was established in adult rabbits. The rabbits were randomly divided into four groups, except the control group, the other three groups were treated with bloodletting and nitroglycerin respectively. Propofol reduced blood pressure by 20% to 30% and maintained it for 20 minutes. Blood samples were collected at 7.5h 24 hours after restoration of blood pressure, and supernatants were obtained by centrifugation. Serum NSE,S-100 尾 protein was determined by enzyme linked immunosorbent assay (ELISA), and the degree of brain injury was compared. Results: compared with the control group, the values of NSE and S-100 尾 in blood samples of three hypotensive groups were all increased at each observation time point, the difference was statistically significant (P0.05). The expression of NSE and S-100 尾 in propofol group were (1487 鹵19) 渭 g / L and (592 鹵10) 渭 g / L at 7.5 h after hypotension, respectively. The expression of S-100 尾 protein was significantly lower than that of the other two experimental groups at 7.5 h (P0.05). Conclusion: blood letting, nitroglycerin and anesthetic drugs can increase the expression of NSE and S-100 尾 protein in bilateral carotid artery stenosis model, especially in propofol group. It may be related to the brain protection of propofol.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R651.15

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