【摘要】：目的：通過(guò)制作大鼠全腦缺血模型，比較再灌注后不同時(shí)間給予純氧對(duì)腦損傷的影響，從而探索腦缺血再灌注后給予高濃度氧的安全時(shí)間范圍。 方法：健康SD雄性大鼠48只隨機(jī)分成6組：假手術(shù)組（Sham，n=8，吸入空氣），純氧組（n=8，再灌注后立即給予純氧1h），純氧一組（n=8，再灌注0.5h后給予純氧1h），純氧二組（n=8，再灌注1h后給予純氧1h），純氧三組（n=8，再灌注2h后給予純氧1h），空氣組（n=8，，一直吸入空氣）。參照Pulsinelli四血管阻斷法制作大鼠全腦缺血再灌注模型，全腦缺血15分鐘。在缺血前（0H），再灌注后(0.5H，1H，2H，4H)采右側(cè)頸內(nèi)靜脈血，離心取血清，測(cè)定異構(gòu)前列腺素（8-iso-PGF2α）、S-100B濃度；再灌注24小時(shí)后斷頭取腦，沿中線將大腦分半，一半測(cè)定腦水含量，另一半4%多聚甲醛固定，制成石蠟切片，通過(guò)TUNEL染色觀察海馬CA1區(qū)神經(jīng)元損傷情況。 結(jié)果：純氧組和純氧一組在給予純氧后，頸內(nèi)靜脈8-iso-PGF2α、S-100B蛋白水平及神經(jīng)元凋亡百分比、腦水含量均比空氣組高，差異具有統(tǒng)計(jì)學(xué)意義；純氧二組在給予純氧后，除S-100B水平，其他指標(biāo)與空氣組有統(tǒng)計(jì)學(xué)差異，且除異構(gòu)前列腺素，其他指標(biāo)與純氧三組無(wú)統(tǒng)計(jì)學(xué)差異；純氧三組上述指標(biāo)與空氣組均無(wú)統(tǒng)計(jì)學(xué)差異。 結(jié)論：在全腦缺血再灌注早期（1小時(shí)內(nèi)）給予純氧會(huì)通過(guò)加強(qiáng)氧化應(yīng)激反應(yīng)而加重腦損傷，而在再灌注2小時(shí)后再給予可能影響較小。
[Abstract]:Aim: to study the effect of pure oxygen on brain injury in rats with global cerebral ischemia and to explore the safe time range of high concentration oxygen after cerebral ischemia reperfusion. Methods: Forty-eight healthy SD male rats were randomly divided into six groups: sham operation group (Sham,n=8, inhaled air), pure oxygen group (n = 8), pure oxygen group (n = 1 h after 0.5 h reperfusion), pure oxygen group (n = 8), pure oxygen group (n = 8). Pure oxygen group 2 (nil 8, 1 h after reperfusion), pure oxygen group 3 (na 8, 2 h after reperfusion for 1 h), air group (n 8, inhaled air all the time). The rat model of global cerebral ischemia reperfusion was established by Pulsinelli four-vessel occlusion method, and the whole brain was ischemia for 15 minutes. Before ischemia (0H) and after reperfusion (0.5H), the right jugular vein blood was collected and the serum was centrifuged to determine the concentration of isoprostaglandin (8-iso-PGF2 偽) and S-100B; After 24 hours of reperfusion, the head was cut off, the brain was divided into half along the midline, the water content in the brain was measured in half, and the other half was fixed with 4% paraformaldehyde. The brain was made into paraffin sections. The neuronal damage in the CA1 area of hippocampus was observed by TUNEL staining. Results: the levels of 8-iso-PGF2 偽, S-100B protein and the percentage of neuronal apoptosis in the jugular vein of the pure oxygen group and the pure oxygen group were higher than those in the air group, and the difference was statistically significant. After pure oxygen was given, the other indexes were significantly different from those of the air group except S-100B, and there was no significant difference between the other indexes and the pure oxygen group except isomeric prostaglandins. There was no statistical difference between the above indexes of pure oxygen group and air group. Conclusion: at the early stage (within 1 hour) of global cerebral ischemia-reperfusion, pure oxygen may aggravate the brain injury by strengthening the oxidative stress response, but it may have little effect after 2 hours of reperfusion.
【學(xué)位授予單位】：華中科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R651.15

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