黃芪對膿毒癥大鼠腸道相關(guān)淋巴細(xì)胞凋亡的影響
發(fā)布時間：2018-11-14 14:27
【摘要】：目的： 觀察黃芪對膿毒癥大鼠腸道相關(guān)淋巴細(xì)胞凋亡的影響。 方法： 選擇使用盲腸結(jié)扎并穿孔術(shù)復(fù)制膿毒癥大鼠模型，將健康雄性SD大鼠72只（25020）g，隨機(jī)分為3組，假手術(shù)組、黃芪治療組和膿毒癥組，在造模成功后的12h、24h、72h三個時間點(diǎn)分別留取腸集合淋巴結(jié)(PP結(jié)）、回盲部上段小腸及小腸粘液灌洗液，采用TUNEL檢測PP結(jié)淋巴細(xì)胞的凋亡率；ELISA測定腸灌洗液IgA含量；免疫組化法觀察腸固有層sIgA陽性細(xì)胞數(shù)量的變化以及PP結(jié)內(nèi)淋巴細(xì)胞Caspase-3蛋白的陽性表達(dá)情況。 結(jié)果： 1、PP結(jié)淋巴細(xì)胞凋亡率 假手術(shù)組在12h、24h及72h各時間點(diǎn)PP結(jié)內(nèi)淋巴細(xì)胞均存在較低的凋亡率無顯著差異（P＞0.05）；膿毒癥組及黃芪治療組在12h、24h、72h各時間點(diǎn)的PP結(jié)淋巴細(xì)胞凋亡率較假手術(shù)組均升高（P＜0.05），12h最高，此后逐步降低，72h最低但較假手術(shù)組仍明顯升高（P＜0.05），且在各時間點(diǎn)膿毒癥組較黃芪治療組也有所升高（P＜0.05），差異有統(tǒng)計學(xué)意義。 2、腸固有層sIgA陽性細(xì)胞數(shù)量的變化 假手術(shù)組在12h、24h及72h三個時間點(diǎn)的腸固有層sIgA陽性細(xì)胞數(shù)量變化無顯著差異（P＞0.05）；膿毒癥組及黃芪治療組在12h、24h、72h各時間點(diǎn)的腸固有層sIgA陽性細(xì)胞數(shù)量較假手術(shù)組均減少（P＜0.05），12h最少，此后逐步增加，72h最多與假手術(shù)組對比仍減少（P＜0.05），且在各時間點(diǎn)膿毒癥組的腸固有層sIgA陽性細(xì)胞數(shù)量也少于黃芪注射液治療組（P＜0.05），差異有統(tǒng)計學(xué)意義。 3、PP結(jié)內(nèi)淋巴細(xì)胞Caspase-3蛋白的陽性表達(dá)情況 假手術(shù)組在12h、24h和72h各個時間點(diǎn)的PP結(jié)淋巴細(xì)胞Caspase-3蛋白均有較低的陽性表達(dá)，各時間點(diǎn)的積分統(tǒng)計無統(tǒng)計學(xué)意義（P＞0.05）；膿毒癥組及黃芪治療組在12h、24h及72h各時間點(diǎn)的PP結(jié)淋巴細(xì)胞Caspase-3蛋白陽性表達(dá)的積分與假手術(shù)組對比，，均顯著增高(P＜0.05)，12h最高，此后逐步降低，72h最低但仍高出數(shù)倍（P＜0.05），且在各時間點(diǎn)膿毒癥組較黃芪治療組增高更加明顯（P＜0.05），差異有統(tǒng)計學(xué)意義。 4、腸灌洗液IgA水平的變化 假手術(shù)組在12h、24h及72h三個時間點(diǎn)的腸灌洗液IgA濃度比較無顯著差異（P＞0.05）；膿毒癥組及黃芪治療組在12h、24h、72h各時間點(diǎn)的腸IgA水平均顯著低于假手術(shù)組（P＜0.05），12h最低，此后逐步升高，72h最高與假手術(shù)組對比仍明顯下降（P＜0.05），且在各時間點(diǎn)膿毒癥組較黃芪治療組也有所降低（P＜0.05），差異有統(tǒng)計學(xué)意義。 結(jié)論： 黃芪能有效抑制膿毒癥大鼠腸道相關(guān)淋巴細(xì)胞的過度凋亡，保護(hù)腸道免疫屏障，具有改善膿毒癥大鼠的腸道黏膜免疫功能。
[Abstract]:Objective: to observe the effect of astragalus on apoptosis of intestinal associated lymphocytes in septic rats. Methods: sepsis rat model was induced by cecal ligation and perforation. 72 healthy male SD rats (25020) g were randomly divided into 3 groups: sham operation group, astragalus membranaceus treatment group and sepsis group. At 72 h, the PP nodes were collected respectively, and the upper ileocecal small intestine and mucus lavage fluid were used to detect the apoptosis rate of PP node lymphocytes by TUNEL. ELISA was used to determine the content of IgA in the intestinal lavage fluid, and the changes of sIgA positive cells in the lamina propria and the expression of Caspase-3 protein in the PP node were observed by immunohistochemical method. Results: 1 there was no significant difference in apoptosis rate of lymphocytes in PP knots between 12h and 72h in sham operation group (P > 0. 05). The apoptosis rate of PP knots in sepsis group and astragalus membranaceus treatment group was higher than that in sham-operated group at 24 h and 72 h (P < 0. 05), the highest at 12 h, and then decreased gradually. The lowest value in 72 hours was higher than that in sham operation group (P < 0.05), and the sepsis group was higher than that of Astragalus membranaceus group at every time point (P < 0.05), and the difference was statistically significant. 2, the change of the number of sIgA positive cells in lamina propria of intestine; there was no significant difference in the number of sIgA positive cells in the lamina propria of intestine at 24 and 72 hours after sham-operation (P > 0. 05). The number of sIgA positive cells in intestinal lamina propria of sepsis group and astragalus membranaceus treatment group at 24 h and 72 h were lower than that in sham operation group (P < 0. 05), and at 12 h, the number of sIgA positive cells in lamina propria was increased gradually. At 72 hours, the number of sIgA positive cells in intestinal lamina propria in sepsis group was lower than that in Huangqi injection group (P < 0. 05). 3The expression of Caspase-3 protein in lymphocytes of PP knots was lower in sham-operated group at 24 h and 72 h after operation, and the expression of Caspase-3 protein in lymphocytes of PP node was lower than that in sham operation group at 24 h and 72 h, respectively. There was no statistical significance in integral statistics at each time point (P > 0.05). In sepsis group and astragalus membranaceus treatment group, the positive expression of Caspase-3 protein in PP node lymphocytes at 12h and 72h was significantly higher than that in sham operation group (P < 0. 05), the highest at 12 h, and then decreased gradually. 72h was the lowest but still several times higher (P < 0.05), and at each time point the sepsis group was higher than the astragalus treatment group (P < 0.05), the difference was statistically significant. 4, the change of IgA level of intestinal lavage fluid in sham-operated group, there was no significant difference in IgA concentration of intestinal lavage fluid at 24 h and 72 h in sham operation group (P > 0. 05). The intestinal IgA levels in sepsis group and astragalus membranaceus treatment group were significantly lower than those in sham operation group (P < 0. 05) at 12 h and 24 h / 72 h, the lowest at 12 h, and then increased gradually. The highest level at 72 h was still significantly lower than that in sham operation group (P < 0. 05). At each time point, sepsis group was lower than that of astragalus treatment group (P < 0.05), the difference was statistically significant. Conclusion: Astragalus membranaceus can effectively inhibit the excessive apoptosis of intestinal lymphocytes and protect the intestinal immune barrier in septic rats. It can improve the intestinal mucosal immune function of septic rats.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R459.7
本文編號：2331423
[Abstract]:Objective: to observe the effect of astragalus on apoptosis of intestinal associated lymphocytes in septic rats. Methods: sepsis rat model was induced by cecal ligation and perforation. 72 healthy male SD rats (25020) g were randomly divided into 3 groups: sham operation group, astragalus membranaceus treatment group and sepsis group. At 72 h, the PP nodes were collected respectively, and the upper ileocecal small intestine and mucus lavage fluid were used to detect the apoptosis rate of PP node lymphocytes by TUNEL. ELISA was used to determine the content of IgA in the intestinal lavage fluid, and the changes of sIgA positive cells in the lamina propria and the expression of Caspase-3 protein in the PP node were observed by immunohistochemical method. Results: 1 there was no significant difference in apoptosis rate of lymphocytes in PP knots between 12h and 72h in sham operation group (P > 0. 05). The apoptosis rate of PP knots in sepsis group and astragalus membranaceus treatment group was higher than that in sham-operated group at 24 h and 72 h (P < 0. 05), the highest at 12 h, and then decreased gradually. The lowest value in 72 hours was higher than that in sham operation group (P < 0.05), and the sepsis group was higher than that of Astragalus membranaceus group at every time point (P < 0.05), and the difference was statistically significant. 2, the change of the number of sIgA positive cells in lamina propria of intestine; there was no significant difference in the number of sIgA positive cells in the lamina propria of intestine at 24 and 72 hours after sham-operation (P > 0. 05). The number of sIgA positive cells in intestinal lamina propria of sepsis group and astragalus membranaceus treatment group at 24 h and 72 h were lower than that in sham operation group (P < 0. 05), and at 12 h, the number of sIgA positive cells in lamina propria was increased gradually. At 72 hours, the number of sIgA positive cells in intestinal lamina propria in sepsis group was lower than that in Huangqi injection group (P < 0. 05). 3The expression of Caspase-3 protein in lymphocytes of PP knots was lower in sham-operated group at 24 h and 72 h after operation, and the expression of Caspase-3 protein in lymphocytes of PP node was lower than that in sham operation group at 24 h and 72 h, respectively. There was no statistical significance in integral statistics at each time point (P > 0.05). In sepsis group and astragalus membranaceus treatment group, the positive expression of Caspase-3 protein in PP node lymphocytes at 12h and 72h was significantly higher than that in sham operation group (P < 0. 05), the highest at 12 h, and then decreased gradually. 72h was the lowest but still several times higher (P < 0.05), and at each time point the sepsis group was higher than the astragalus treatment group (P < 0.05), the difference was statistically significant. 4, the change of IgA level of intestinal lavage fluid in sham-operated group, there was no significant difference in IgA concentration of intestinal lavage fluid at 24 h and 72 h in sham operation group (P > 0. 05). The intestinal IgA levels in sepsis group and astragalus membranaceus treatment group were significantly lower than those in sham operation group (P < 0. 05) at 12 h and 24 h / 72 h, the lowest at 12 h, and then increased gradually. The highest level at 72 h was still significantly lower than that in sham operation group (P < 0. 05). At each time point, sepsis group was lower than that of astragalus treatment group (P < 0.05), the difference was statistically significant. Conclusion: Astragalus membranaceus can effectively inhibit the excessive apoptosis of intestinal lymphocytes and protect the intestinal immune barrier in septic rats. It can improve the intestinal mucosal immune function of septic rats.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R459.7
【參考文獻(xiàn)】
相關(guān)期刊論文 前10條
1 袁芳芳;蘇磊;劉志鋒;;腸功能障礙分子機(jī)制的研究進(jìn)展[J];廣東醫(yī)學(xué);2012年12期
2 莊艷;劉先領(lǐng);;蒽環(huán)類藥物心臟毒性及其預(yù)防措施[J];腫瘤藥學(xué);2011年04期
3 王瑩;李文媛;鄭海星;佟曉杰;;黃芪皂苷Ⅳ對大鼠腦缺血/再灌注損傷海馬神經(jīng)元凋亡及相關(guān)基因表達(dá)的影響[J];中國臨床保健雜志;2011年04期
4 豐成相;;黃芪中主要化學(xué)成分的藥理學(xué)研究概況[J];中國民族民間醫(yī)藥;2012年03期
5 李淑芳;;中藥黃芪藥理作用研究進(jìn)展[J];湖北中醫(yī)雜志;2013年06期
6 孟啟勇;向鏡芬;;免疫功能改變對膿毒癥患者預(yù)后的影響[J];湖南師范大學(xué)學(xué)報(醫(yī)學(xué)版);2013年02期
7 趙劭懂;葛許華;蔡愛東;江濤;喻文亮;;T細(xì)胞亞群選擇性缺失對小兒膿毒癥發(fā)生發(fā)展的影響[J];實(shí)用兒科臨床雜志;2010年18期
8 王秋寧;王洪新;楊育紅;魯美麗;李香華;;黃芪甲苷對異丙腎上腺素誘導(dǎo)的乳大鼠心肌細(xì)胞肥大的保護(hù)作用[J];中國藥理學(xué)與毒理學(xué)雜志;2011年01期
9 閆鳳霞;錢濤;高維娟;葉冬青;任立群;張雅麗;侯志平;;黃芪注射液對缺氧缺糖/復(fù)氧復(fù)糖大鼠海馬神經(jīng)元鈣調(diào)蛋白表達(dá)的影響[J];中國病理生理雜志;2011年09期
10 趙瑞杰;李引乾;王會;王廣彬;娜日蘇;金大鵬;關(guān)偉軍;馬月輝;;Caspase家族與細(xì)胞凋亡的關(guān)系[J];中國畜牧雜志;2010年17期
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