發(fā)布時(shí)間：2018-11-10 23:11

【摘要】：目的:探討骨髓間質(zhì)干細(xì)胞(bone marrow mesenchymal stem cells,BM-MSCs)對(duì)大鼠顱腦損傷后血管再生的影響。方法:自由落體法建立大鼠腦撞擊傷模型,50只Sprague-Dawley大鼠隨機(jī)分為移植組和對(duì)照組,每組25只。移植組大鼠手術(shù)后12 h側(cè)腦室注射法移植BM-MSCs,對(duì)照組僅注射生理鹽水。術(shù)后第1,3,7,14和21天行改良大鼠神經(jīng)功能缺損評(píng)分(modified neurological deficit scores of rats,mNSS)。于術(shù)前,術(shù)后3,6,12,24 h和3,7 d采用流式細(xì)胞儀檢測大鼠外周血CD34和CD133抗體雙標(biāo)記細(xì)胞。免疫組織化學(xué)SP法檢測損傷周圍腦組織CD31和神經(jīng)元特異性烯醇化酶(neuron-specific enolase,NSE)的表達(dá)。結(jié)果:兩組間mNSS分值差異有統(tǒng)計(jì)學(xué)意義(F=5.997,P0.05),組內(nèi)不同時(shí)間點(diǎn)間差異也有統(tǒng)計(jì)學(xué)意義(F=37.106,P0.01)。術(shù)后第7,14,21天對(duì)照組較移植組mNSS評(píng)分高,差異有統(tǒng)計(jì)學(xué)意義(均P0.05)。大鼠外周血中存在CD34和CD133雙陽性細(xì)胞表達(dá)。對(duì)照組大鼠外周血中CD34和CD133雙陽性細(xì)胞數(shù)傷后3 h為下降狀態(tài),后升高,傷后6 h達(dá)最高點(diǎn),此后逐漸下降,傷后24 h降至正常水平。移植組有同樣的趨勢,CD34和CD133雙陽性細(xì)胞升高持續(xù)到傷后24 h,其數(shù)量明顯高于對(duì)照組(P0.05)。術(shù)前兩組NSE均有陽性表達(dá),術(shù)后7,14 d時(shí)移植組的NSE表達(dá)顯著高于對(duì)照組(P0.05)。術(shù)前兩組CD31的陽性表達(dá)很少,術(shù)后3,7 d時(shí)移植組的CD31表達(dá)顯著高于對(duì)照組(均P0.05)。結(jié)論:BM-MSCs移植可以增加腦創(chuàng)傷后大鼠外周血中內(nèi)皮祖細(xì)胞數(shù)量并持續(xù)約24 h,可以調(diào)高大鼠腦創(chuàng)傷后損傷周圍區(qū)的血管生成標(biāo)志物的表達(dá)和神經(jīng)元標(biāo)志物的表達(dá)。BM-MSCs移植組大鼠腦損傷后神經(jīng)功能較對(duì)照組改善明顯。
[Abstract]:Objective: to investigate the effect of bone marrow mesenchymal stem cells (bone marrow mesenchymal stem cells,BM-MSCs) on vascular regeneration after craniocerebral injury in rats. Methods: the rat model of brain impact injury was established by free-fall method. Fifty Sprague-Dawley rats were randomly divided into transplantation group and control group with 25 rats in each group. Rats in the transplantation group received intraventricular injection of saline 12 hours after operation. The modified neurological deficit score (modified neurological deficit scores of rats,mNSS) was performed on the 1st day, 3rd day, 14 th and 21 th day after operation. CD34 and CD133 antibody double labeled cells in peripheral blood of rats were detected by flow cytometry at 24 hours and 3 days after operation. The expression of CD31 and neuron-specific enolase (neuron-specific enolase,NSE) were detected by immunohistochemical SP method. Results: there was significant difference in mNSS score between the two groups (P 0.05), and there was also significant difference between the two groups at different time points (P 0.01). The mNSS score of the control group was significantly higher than that of the transplantation group on day 7, 14 and 21 (P0.05). CD34 and CD133 double positive cells were expressed in peripheral blood of rats. In the control group, the number of CD34 and CD133 double positive cells in peripheral blood decreased 3 h after injury, then increased, reached the peak at 6 h after injury, then gradually decreased, and then decreased to normal level 24 h after injury. There was the same trend in the transplantation group, CD34 and CD133 double positive cells increased until 24 hours after injury, the number of the cells was significantly higher than that of the control group (P0.05). There was positive expression of NSE in both groups before operation, and the expression of NSE in transplantation group was significantly higher than that in control group at 714 days postoperatively (P0.05). The expression of CD31 in the transplantation group was significantly higher than that in the control group 3 days after operation (P0.05). Conclusion: BM-MSCs transplantation can increase the number of endothelial progenitor cells in peripheral blood of rats after brain trauma for 24 hours. The expression of angiogenic markers and neuronal markers in the peritraumatic area of high and high brain injury could be regulated. The nerve function in the BM-MSCs transplantation group was significantly improved than that in the control group.
【作者單位】： 九江市第一人民醫(yī)院神經(jīng)外科;
【基金】：江西省衛(wèi)生廳科技基金(20131736)~~
【分類號(hào)】：R651.15

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