【摘要】：目的：探討黃體酮對蛛網(wǎng)膜下腔出血(subarachnoid hemorrhage,SAH)后早期腦損傷(early brain injury,EBI)中細胞凋亡、血腦屏障(blood-brain barrier,BBB)的穩(wěn)定性、腦水腫和病死率保護作用。 方法：66只大鼠被隨機分為假手術(shù)組,SAH+溶劑組,SAH+黃體酮組。于SAH模型制成后1、6h和12h分別給予黃體酮(16mg/kg)或等體積的溶劑。在SAH后24h分析不同組間大鼠在病死率、神經(jīng)功能評分、腦水腫、細胞凋亡、caspase-3及基質(zhì)金屬蛋白酶-9(MMP-9)表達水平的差異。 結(jié)果：與SAH+溶劑組比較,黃體酮治療顯著降低了大鼠的病死率、細胞凋亡程度以及caspase-3水平與MMP-9的表達水平,減輕了腦水腫和伊文思藍的滲出,提高了神經(jīng)功能評分(P0.05)。 結(jié)論：黃體酮可通過抑制細胞凋亡和穩(wěn)定BBB減輕SAH后EBI。
[Abstract]:Aim: to investigate the effects of progesterone on apoptosis, stability of blood-brain barrier (blood-brain barrier,BBB), cerebral edema and mortality in early brain injury (early brain injury,EBI) after subarachnoid hemorrhage (subarachnoid hemorrhage,SAH). Methods: 66 rats were randomly divided into sham-operation group, SAH solvent group and SAH progesterone group. Progesterone (16mg/kg) or the same volume of solvent was given at 1h and 12h after the SAH model was made. 24 hours after SAH, the differences of mortality, neurological function score, brain edema, apoptosis, caspase-3 and matrix metalloproteinase-9 (MMP-9) expression in different groups were analyzed. Results: compared with SAH solvent group, progesterone significantly decreased the mortality, apoptosis degree, caspase-3 level and MMP-9 expression, reduced brain edema and Evans blue exudation, and increased the neurological function score (P0.05). Conclusion: progesterone can reduce EBI. after SAH by inhibiting apoptosis and stabilizing BBB.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R651.15

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相關(guān)期刊論文 前2條

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本文編號：2269032