【摘要】：目的：構(gòu)建心臟驟停-心肺復(fù)蘇成功的大鼠模型,進(jìn)一步研究亞低溫和烏司他丁聯(lián)合治療對(duì)心肺復(fù)蘇大鼠腦組織的保護(hù)作用。 方法：采用窒息法制備大鼠的心肺復(fù)蘇模型。隨機(jī)分成五組：假手術(shù)組、對(duì)照組、亞低溫組、烏司他丁藥物組、烏司他丁聯(lián)合亞低溫組,治療結(jié)束后于1、3、6小時(shí)各取血液2ml,通過ELISA分別檢測(cè)各組血液中炎癥細(xì)胞因子TNF-α、IL-6、IL-8的表達(dá)。手術(shù)6小時(shí)后處死大鼠,取腦組織,光鏡檢測(cè)腦組織病理形態(tài)變化。 結(jié)果：(1)血清中炎癥因子表達(dá)量：與對(duì)照組比較,亞低溫組、烏司他丁組、聯(lián)合組在短時(shí)間內(nèi)(3小時(shí)內(nèi))均可抑制IL-6,IL-8,TNF-α的表達(dá)且效果顯著(P0.05),聯(lián)合組的抑制效果更為明顯。當(dāng)時(shí)間延長(zhǎng)到6小時(shí),聯(lián)合組抑制效果與亞低溫組或?yàn)跛舅〗M差異無統(tǒng)計(jì)學(xué)意義(P0.05)。(2)腦組織病理形態(tài)變化：心肺復(fù)蘇后各組均有不同程度的腦部損傷,亞低溫組、烏司他丁組、腦部損傷程度有所減緩,聯(lián)合組損傷程度最輕。 結(jié)論：烏司他丁和亞低溫的聯(lián)合應(yīng)用可抑制心肺復(fù)蘇術(shù)后大鼠的炎癥反應(yīng),減緩腦損傷。圖6幅,表4個(gè),參考文獻(xiàn)65篇
[Abstract]:Aim: to establish a successful rat model of cardiac arrest and cardiopulmonary resuscitation (CPR) and to study the protective effects of mild hypothermia and ulinastatin on brain tissue of cardiopulmonary resuscitation (CPR) rats. Methods: the rat cardiopulmonary resuscitation model was established by asphyxia. They were randomly divided into five groups: sham operation group, control group, mild hypothermia group, ulinastatin drug group, ulinastatin combined with mild hypothermia group. The blood samples were collected at 1, 3 and 6 hours after treatment. The expression of inflammatory cytokines TNF- 偽 and IL-6,IL-8 in the blood of each group were detected by ELISA. The rats were killed 6 hours after operation, the brain tissue was taken and the pathological changes of brain tissue were detected by light microscope. Results: (1) the expression of inflammatory factors in serum: compared with the control group, the mild hypothermia group, ulinastatin group and combined group could inhibit the expression of IL-6,IL-8,TNF- 偽 in a short time (3 hours) and the effect was significant (P0.05), the inhibitory effect of the combined group was more obvious than that of the control group. When the time was prolonged to 6 hours, there was no significant difference in the inhibitory effect between the combined group and mild hypothermia group or ulinastatin group (P0.05). (2). After cardiopulmonary resuscitation, there were different degrees of brain injury in the combined group, mild hypothermia group and ulinastatin group. The degree of brain injury was alleviated, and that of the combined group was the least. Conclusion: the combination of ulinastatin and mild hypothermia can inhibit the inflammatory response of rats after cardiopulmonary resuscitation and slow down brain injury. 6 figures, 4 tables, 65 references
【學(xué)位授予單位】：中南大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R459.7

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