自噬在急性心肌梗死后無(wú)復(fù)流中的表達(dá)
發(fā)布時(shí)間：2018-08-30 12:10
【摘要】：目的觀察急性心肌梗死后無(wú)復(fù)流區(qū)心肌細(xì)胞自噬的表達(dá),探討心肌細(xì)胞自噬在大鼠實(shí)驗(yàn)性無(wú)復(fù)流中的作用。方法 SD大鼠隨機(jī)分為實(shí)驗(yàn)組和對(duì)照組:均予結(jié)扎冠狀動(dòng)脈2 h,再灌注1 h,建立急性心肌梗死后再灌注無(wú)復(fù)流模型,對(duì)照組只穿線,不結(jié)扎。在再灌注末,從左心房注入6%硫磺素1 m L/kg使復(fù)流區(qū)著色,無(wú)復(fù)流區(qū)不著色;再于原位重新結(jié)扎冠狀動(dòng)脈,實(shí)驗(yàn)組從左心房注入Evan'S藍(lán),使結(jié)扎區(qū)外著藍(lán)色,梗死區(qū)不著藍(lán)色,對(duì)照組不予Evan's藍(lán)。采用實(shí)時(shí)定量PCR法和Western blot法檢測(cè)實(shí)驗(yàn)組無(wú)復(fù)流區(qū)、梗死區(qū)和正常區(qū)心肌組織自噬相關(guān)蛋白LC3、p62/SQSTM1表達(dá);電鏡下觀察自噬體超微結(jié)構(gòu)改變情況。結(jié)果 TTC和硫磺素染色結(jié)果顯示,實(shí)驗(yàn)組無(wú)復(fù)流面積顯著高于對(duì)照組(P0.05);透射電鏡觀察到,大鼠心肌細(xì)胞內(nèi)自噬體體積增大,數(shù)量增多;PCR法和Western blot法檢測(cè)實(shí)驗(yàn)組無(wú)復(fù)流區(qū)、梗死區(qū)及正常區(qū)心肌組織自噬相關(guān)蛋白LC3-Ⅰ、LC3-Ⅱ,p62/SQSTM1的表達(dá),實(shí)驗(yàn)組無(wú)復(fù)流區(qū)自噬表達(dá)明顯高于梗死區(qū)和正常區(qū)心肌組織(P0.05),但梗死區(qū)和遠(yuǎn)端區(qū)心肌組織自噬表達(dá)差異無(wú)統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論實(shí)驗(yàn)組無(wú)復(fù)流區(qū)心肌組織局部自噬表達(dá)增強(qiáng),其活性的改變可能與無(wú)復(fù)流的發(fā)展及程度相關(guān)。
[Abstract]:Objective to observe the expression of myocardial autophagy in non-reflow zone after acute myocardial infarction (AMI) and to investigate the role of myocardial autophagy in experimental non-reflow rats. Methods SD rats were randomly divided into experimental group and control group: coronary artery was ligated for 2 hours and reperfusion for 1 hour. At the end of reperfusion, the reflow area was stained by injecting 6% sulfur 1 m L/kg from the left atrium, and the no reflow area was not stained, and then the coronary artery was religated in situ, and the Evan'S blue was injected into the left atrium in the experimental group, so that the ligation area was blue outside the ligation area and the infarcted area was not blue. The control group was not treated with Evan's blue. Real time quantitative PCR and Western blot were used to detect the expression of autophagy associated protein (LC3,p62/SQSTM1) in myocardium without reflow, infarction and normal myocardium, and ultrastructural changes of autophagy were observed under electron microscope. Results the results of TTC and sulfur staining showed that the no reflow area in the experimental group was significantly higher than that in the control group (P0.05). Expression of autophagy associated protein LC3- 鈪，
本文編號(hào)：2213033
[Abstract]:Objective to observe the expression of myocardial autophagy in non-reflow zone after acute myocardial infarction (AMI) and to investigate the role of myocardial autophagy in experimental non-reflow rats. Methods SD rats were randomly divided into experimental group and control group: coronary artery was ligated for 2 hours and reperfusion for 1 hour. At the end of reperfusion, the reflow area was stained by injecting 6% sulfur 1 m L/kg from the left atrium, and the no reflow area was not stained, and then the coronary artery was religated in situ, and the Evan'S blue was injected into the left atrium in the experimental group, so that the ligation area was blue outside the ligation area and the infarcted area was not blue. The control group was not treated with Evan's blue. Real time quantitative PCR and Western blot were used to detect the expression of autophagy associated protein (LC3,p62/SQSTM1) in myocardium without reflow, infarction and normal myocardium, and ultrastructural changes of autophagy were observed under electron microscope. Results the results of TTC and sulfur staining showed that the no reflow area in the experimental group was significantly higher than that in the control group (P0.05). Expression of autophagy associated protein LC3- 鈪，
本文編號(hào)：2213033
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