【摘要】：目的：制作SD大鼠TBI模型并采用雄激素干預(yù)治療，了解雄激素對(duì)創(chuàng)傷性腦損傷(TBI)大鼠腦組織磷酸蛋白聚(phosphacan, PC)及NG2蛋白聚糖(NG2)表達(dá)影響與創(chuàng)傷性腦損傷后神經(jīng)修復(fù)的關(guān)系,探討其促神經(jīng)再生的作用機(jī)制。 方法 1、選擇雄性Sprague-Dawley大鼠48只，體重為140g～200g。隨機(jī)分為正常組、TBI模型組、雄激素干預(yù)組(于模型制成后即刻注射丙酸睪丸酮注射液25mg/kg)。 2、使用改進(jìn)的FEENEY'S法制作SD大鼠TBI模型。 3、造模后24h、72h、5d、7d取腦組織制作石蠟切片，用免疫組化法用Anti-NG2/CSPG4、Anti-PTP zeta/Phosphacan標(biāo)記。觀察損傷區(qū)組織磷酸蛋白聚糖(phosphacan, PC)及NG2蛋白聚糖表（NG2）達(dá)動(dòng)態(tài)變化，并采用灰度分析量化比較。 4、雄激素干預(yù)組造模后制電鏡標(biāo)本應(yīng)用透射電鏡對(duì)比觀察損傷區(qū)及其周?chē)纳窠?jīng)元超微結(jié)構(gòu)變化、細(xì)胞損傷、凋亡、神經(jīng)元軸突再生及膠質(zhì)細(xì)胞增生情況。 結(jié)果 1、雄激素干預(yù)組造模后24h、72h、5d、7d后腦組織損傷區(qū)及周?chē)磉_(dá)水平明顯低于TBI組。 2、雄激素干預(yù)組24h、72h、5d和7d時(shí)細(xì)胞水腫,可見(jiàn)凋亡細(xì)胞，，TBI模型組細(xì)胞表面突起較少或接近與無(wú);雄激素干預(yù)組的神經(jīng)元軸突較TBI模型組顯著增多 3、24h、72h、5d、7d損傷外圍膠質(zhì)細(xì)胞增生情況較TBI模型組明顯降低 結(jié)論 雄激素可使PC和NG2表達(dá)抑制而促進(jìn)神經(jīng)元軸突再生,促進(jìn)神經(jīng)修復(fù)。
[Abstract]:Objective: to investigate the effect of androgen on the expression of phosphatase (PC) and NG2 proteoglycan (NG2) in the brain tissue of rats with traumatic brain injury (TBI) and to investigate the relationship between the effects of androgen on the nerve repair after traumatic brain injury (TBI). To explore the mechanism of promoting nerve regeneration. Methods 1. Forty-eight male Sprague-Dawley rats, weighing 140 g / 200 g, were selected. They were randomly divided into normal group and TBI model group. In the androgen intervention group (testosterone propionate injection 25mg/kg). 2. The SD rat model was made by using the modified FEENEYS method. Anti-NG2 / CSPG4 + Anti-PTP zeta / Phosphacan was labeled by immunohistochemical method. To observe the dynamic changes of phosphate proteoglycan (PC) and NG2 proteoglycan (NG2) in injured tissue. In androgen intervention group, the ultrastructural changes, cell injury and apoptosis of neurons in and around the injured area were observed by transmission electron microscope (TEM). Axon regeneration and glial cell proliferation of neurons. Results 1. The expression level of brain tissue injury area and surrounding area in androgen intervention group was significantly lower than that in TBI group at 24 h, 72 h and 5 d after establishment of the model, and the cell edema was found in androgen intervention group at 24 h, 72 h, 5 d and 7 d, respectively. It can be seen that the apoptotic cells in TBI model group have less or close to none of the surface processes. The neuronal axons in the androgen intervention group were significantly higher than those in the TBI model group. [WT5 "HZ] the proliferation of peripheral glial cells in the androgen intervention group was significantly lower than that in the TBI model group. Conclusion androgen can induce the expression of PC and NG2 in TBI model group. Inhibit and promote neuronal axon regeneration, Promote nerve repair.
【學(xué)位授予單位】：山西醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R651.15

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