【摘要】：目的探討腦室給予CGRP受體拮抗劑CGRP8-37對顱腦創(chuàng)傷后大鼠的保護(hù)作用。方法將90只Wistar大鼠隨機(jī)分為對照組(6只)、創(chuàng)傷組(TBI組,42只)、CGRP8-37組(42只)。TBI組、CGRP8-37組大鼠分別作自由落體創(chuàng)傷模型,應(yīng)用免疫組化和PCR技術(shù),動態(tài)觀察對照、創(chuàng)傷和CGRP8-37組大鼠0.5h,2h,6h,12h,24h,48h,72h后CGRP的變化規(guī)律。結(jié)果①免疫組化顯示:對照組在TBI組腦組織CGRP陽性單位在傷后0.5h開始表達(dá)上調(diào),2h、6h依次增高,12h達(dá)到峰值(P0.05),之后逐漸回落,72h時高于正常水平。與TBI組相比,CGRP8-37組腦組織CGRP陽性單位表達(dá)在2h即達(dá)到高峰,隨后持續(xù)回落,48h即恢復(fù)正常,72h低于正常水平。且在相應(yīng)的時間點(diǎn)CGRP陽性單位表達(dá)都低于TBI組。②PCR顯示:與免疫組化結(jié)果基本相似,即CGRP8-37組腦組織CGRP mRNA表達(dá)較TBI組峰值提前,由12h提前至2h,且在相應(yīng)時間點(diǎn)減少CGRP mRNA表達(dá)。結(jié)論CGRP受體拮抗劑CGRP8-37能減輕大鼠創(chuàng)傷性腦損傷后CGRP的表達(dá),減輕腦水腫,并能促進(jìn)病理過程的恢復(fù)。
[Abstract]:Objective to investigate the protective effect of CGRP receptor antagonist CGRP8-37 on rats with traumatic brain injury. Methods 90 Wistar rats were randomly divided into control group (n = 6), trauma group (n = 42), CGRP8-37 group (n = 42). The changes of CGRP were observed in rats in the trauma and CGRP8-37 groups after 72 hours of injury and 24 h, 24 h and 48 h. Results 1Immunohistochemistry showed that the expression of CGRP positive units in brain tissue of TBI group increased from 0.5 h to 6 h after injury and reached the peak at 12 h (P0.05), then decreased gradually to the normal level at 72 h after injury. Compared with TBI group, the expression of CGRP positive units reached its peak at 2 h, and then returned to normal level at 48 h. At the corresponding time point, CGRP positive unit expression was lower than that in TBI group. 2PCR showed that CGRP mRNA expression in brain tissue of CGRP8-37 group was similar to that of TBI group, that is, CGRP mRNA expression in CGRP8-37 group was earlier than that in TBI group, from 12 h to 2 h, and CGRP mRNA expression was decreased at the corresponding time point. Conclusion CGRP receptor antagonist CGRP8-37 can reduce the expression of CGRP after traumatic brain injury, alleviate brain edema and promote the recovery of pathological process.
【作者單位】： 山西醫(yī)科大學(xué);山西醫(yī)科大學(xué)第一醫(yī)院;
【基金】：國家自然基金資助項(xiàng)目(No.30600637)
【分類號】：R651.1

【共引文獻(xiàn)】

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