本文選題：膿毒癥 + 腦腸肽��； 參考：《蘭州大學(xué)》2013年碩士論文

【摘要】：目的探討外源性腦腸肽Ghrelin對膿毒癥大鼠小腸上皮短肽載體-1表達(dá)及功能的影響。 方法雄性SD大鼠80只,隨機(jī)分為4組,正常組、假手術(shù)組、膿毒癥組和Ghrelin干預(yù)組,每組20只；采用盲腸結(jié)扎穿刺術(shù)模型,制模后立即給予Ghrelin靜脈干預(yù)；模型建立后20h每組隨機(jī)處理10只大鼠,Elisa方法檢測TNF-α、IL-1β和Ghrelin水平,免疫組化、實(shí)時定量PCR和蛋白印跡分別檢測PepTl分布、mRNA和蛋白表達(dá),高效液相色譜法檢測PepTl攝取功能；每組剩余10只大鼠觀察生存時間(7d)。 結(jié)果(1)與正常組和假手術(shù)組相比,膿毒癥時小腸黏膜受損明顯,血清及小腸黏膜TNF-α、IL-1β明顯升高(P0.05)；小腸黏膜PepTl分布減少,PepTl mRNA表達(dá)、蛋白表達(dá)以及對底物攝取能力明顯降低(P0.05)；(2)與膿毒癥組比較,Ghrelin干預(yù)組大鼠小腸黏膜損傷較輕,血清及腸黏膜TNF-α、IL-1β(P0.05);小腸黏膜PepTl分布量增多,生存率、PepTl mRNA表達(dá)蛋白表達(dá)及對底物攝取能力顯著升高(P0.05)；(3)正常組與假手術(shù)組各指標(biāo)比較無顯著差異(P0.05)。 結(jié)論膿毒癥時大鼠小腸上皮PepTl mRNA、蛋白表達(dá)以及PepTl在腸黏膜分布明顯下降,機(jī)體在基因及蛋白水平下調(diào)了小腸上皮PepTl生物學(xué)功能；Ghrelin干預(yù)可降低炎性反應(yīng),對膿毒癥小腸上皮PepTl mRNA、蛋白表達(dá)以及攝取能力均有上調(diào)作用。
[Abstract]:Objective to investigate the effects of exogenous brain gut peptide ghrelin on the expression and function of intestinal epithelial short peptide vector-1 in septic rats. Methods 80 male Sprague-Dawley rats were randomly divided into 4 groups: normal group, sham operation group, sepsis group and ghrelin intervention group with 20 rats in each group. The levels of TNF- 偽 -1 尾 and ghrelin were detected by Elisa, the mRNA and protein expression of PepTL were detected by immunohistochemistry, real-time quantitative PCR and Western blot, respectively. The uptake function of PepTL was detected by high performance liquid chromatography (HPLC). The survival time (7 days) of the remaining 10 rats in each group was observed. Results (1) compared with the normal group and the sham operation group, the intestinal mucosa was damaged significantly in sepsis, the TNF- 偽 -IL-1 尾 in serum and small intestinal mucosa was significantly increased (P0.05), the distribution of PepTl in intestinal mucosa decreased the expression of PepTl mRNA, the protein expression and the ability of uptake of substrate (P0.05). (2) compared with the sepsis group, the intestinal mucosal damage in ghrelin intervention group was lighter, TNF- 偽 -IL-1 尾 in serum and intestinal mucosa increased (P0.05), and PepTl mRNA expression and substrate uptake in small intestinal mucosa increased significantly (P0.05), while the survival rate of ghrelin intervention group was significantly higher than that in sepsis group. (3) there was no significant difference between normal group and sham operation group (P0.05). Conclusion PepTl mRNAs, protein expression and PepTl distribution in intestinal mucosa were significantly decreased in septic rats. The biological function of PepTl in intestinal epithelium was down-regulated by ghrelin intervention at gene and protein level. It can up-regulate PepTl mRNAs, protein expression and uptake ability in septic intestinal epithelium.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R459.7

【參考文獻(xiàn)】

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1 黎介壽;;對腸功能障礙的再認(rèn)識[J];腸外與腸內(nèi)營養(yǎng);2008年06期

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