本文選題：臍帶間充質(zhì)干細(xì)胞 + 移植　； 參考：《天津醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：觀察經(jīng)靜脈或冠脈移植臍帶間充質(zhì)干細(xì)胞(mesenchymal stem cells, MSCs)對(duì)急性心肌梗死豬的心功能、梗死面積、新生毛細(xì)血管數(shù)量的影響。方法：實(shí)驗(yàn)于2011年1月至2012年6月在泰達(dá)國(guó)際心血管病醫(yī)院動(dòng)物實(shí)驗(yàn)室在用球囊封堵前降支制作急性心肌梗死模型。造模成功并完成干細(xì)胞移植的23只豬分為3組：冠脈組8只,靜脈組8只,對(duì)照組7只。移植組于心肌梗死后2周分別經(jīng)靜脈和冠脈的方式移植干細(xì)胞,對(duì)照組經(jīng)靜脈注射等量的生理鹽水。所有實(shí)驗(yàn)豬分別于干細(xì)胞移植前和移植后6周行超聲心動(dòng)圖檢查,測(cè)量左室舒張末容積(LVEDV).左室收縮末容積(LVESV)、左心室射血分?jǐn)?shù)(LVEF)、短軸縮短率(FS)；所有實(shí)驗(yàn)豬分別于干細(xì)胞移植前和移植后6周行心肌核素灌注顯像檢查,計(jì)算左室梗死面積及梗死質(zhì)量比；移植后6周時(shí),氯化鉀注射法處死豬,分別取梗死區(qū)、梗死周邊區(qū)心肌,行vWF免疫組化染色,計(jì)算毛細(xì)血管密度。并行透射電鏡觀察細(xì)胞移植后分化情況。 結(jié)果： 1.與對(duì)照組相比,靜脈移植組和冠脈移植組的左室舒張末容積(LVEDV)、左室收縮末容積(LVESV)縮小、左室射血分?jǐn)?shù)(LVEF)、短軸縮短率(FS)增加,差異具有統(tǒng)計(jì)學(xué)意義[冠脈組：56.86±7.62mm,16.25±6.45mm,71.25±8.94%,39.52±6.85%;靜脈組：59.38±5.55mm,17.63±6.14mm,69.38±6.93%,38.25±6.69%;對(duì)照組：70.57±8.50mm,25.57±7.55mm,59.71±7.18%,31.29±5.19%;(P0.05)]。冠脈移植組與靜脈移植組比較,無(wú)明顯差異(P0.05)。 2.與對(duì)照組相比,冠脈組和靜脈組的左室心肌梗死面積、梗死質(zhì)量比明顯縮小,差異具有統(tǒng)計(jì)學(xué)意義[冠脈組：15.13±2.99%,16.13±2.99%；靜脈組：13.13±3.23%,15.00±3.07%；對(duì)照組：21.43±5.88%,22.86±6.49%；(P0.05)]。冠脈組與靜脈組間無(wú)顯著性差異(P0.05)。 3.與對(duì)照組相比,冠脈組和靜脈組的新生毛細(xì)血管的數(shù)量明顯增加,差異具有統(tǒng)計(jì)學(xué)意義[冠脈組：14.71±2.99個(gè)；靜脈組：13.58±2.65個(gè)；對(duì)照組：10.24±1.71個(gè)；(P0.05)]。冠脈組與靜脈組間無(wú)顯著性差異(P0.05)。 4.移植組臍帶間充質(zhì)干細(xì)胞可分布于梗死區(qū)及梗死周邊區(qū),透射電鏡下可見(jiàn)由干細(xì)胞分化而來(lái)的的血管內(nèi)皮細(xì)胞及心肌樣細(xì)胞。 結(jié)論：經(jīng)靜脈或冠脈兩種途徑移植臍帶MSCs均能有效地改善急性心肌梗死豬的心功能,縮小左室舒張末期容積和收縮末期容積,縮小梗死面積,增加毛細(xì)血管的數(shù)量,兩種途徑之間無(wú)顯著的差異。
[Abstract]:Aim: to investigate the effects of mesenchymal stem cells (MSCs) on cardiac function, infarct size and the number of new capillaries in acute myocardial infarction (AMI) pigs. Methods: from January 2011 to June 2012, the model of acute myocardial infarction was established in the animal laboratory of Taida International Cardiovascular Hospital. The 23 pigs were divided into 3 groups: coronary group (n = 8), vein group (n = 8) and control group (n = 7). Stem cells were transplanted by vein and coronary artery two weeks after myocardial infarction in the transplantation group and the control group were injected with the same amount of physiological saline via vein. The left ventricular end-diastolic volume (LVEF) was measured by echocardiography before and 6 weeks after stem cell transplantation. Left ventricular end-systolic volume (LVEF), left ventricular ejection fraction (LVEF), short axis shortening rate (STR) and myocardial radionuclide perfusion imaging were performed before and 6 weeks after transplantation to calculate the infarct size and infarct mass ratio of the left ventricle. The pigs were killed by potassium chloride injection. The infarcted and peri-infarcted myocardium were taken respectively and the capillary density was calculated by using vWF immunohistochemical staining. The cell differentiation after transplantation was observed by transmission electron microscope. Results: 1. Compared with the control group, left ventricular end-diastolic volume (LVEF), left ventricular end-systolic volume (LVESVV), left ventricular ejection fraction (LVEF) and short axis shortening rate (FSs) were decreased in vein transplantation group and coronary artery transplantation group. The difference was statistically significant [56.86 鹵7.62mm 鹵16.25 鹵6.45mm in the coronary group 71.25 鹵8.94 in the coronary group, 39.52 鹵6.85mm in the venous group; 59.38 鹵5.55mm in the venous group, 69.38 鹵6.93mm in the venous group, 38.38 鹵6.93mm in the control group; 70.57 鹵8.50mm / 25.57 鹵7.55mm / 25.57 鹵7.55mm / 59.71 鹵7.18mm in the control group, 31.29 鹵5.195mm respectively]. There was no significant difference between the coronary artery transplantation group and the vein transplantation group. Compared with the control group, the myocardial infarction size and the infarct mass ratio of the coronary artery group and the vein group were significantly reduced, and the difference was statistically significant [the coronary artery group: 15.13 鹵2.99g; the venous group: 13.13 鹵3.23g 15.00 鹵3.07; the control group 21.43 鹵5.880.86 鹵6.49P 0.05]. There was no significant difference between the coronary artery group and the venous group. Compared with the control group, the number of neocapillaries in the coronary artery group and the venous group was significantly increased, with statistical significance [the coronary artery group: 14.71 鹵2.99; the venous group: 13.58 鹵2.65; the control group: 10.24 鹵1.71 (P0.05)]. There was no significant difference between the coronary artery group and the venous group. The umbilical cord mesenchymal stem cells in the transplantation group could be distributed in the infarcted and peri-infarction areas. Vascular endothelial cells and cardiomyocyte-like cells differentiated from stem cells could be seen under transmission electron microscope. Conclusion: transplantation of umbilical cord MSCs via vein or coronary artery can effectively improve cardiac function, reduce left ventricular end-diastolic volume and end-systolic volume, reduce infarct area and increase the number of capillaries in acute myocardial infarction pigs. There was no significant difference between the two approaches.
【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R542.22

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陸東風(fēng);吳昊;黃t，

本文編號(hào)：2041389