依那普利拉對嚴(yán)重?zé)齻笫笮募p傷的保護(hù)作用及其機(jī)制探討
發(fā)布時間：2018-06-19 20:21
本文選題：依那普利拉 + 嚴(yán)重?zé)齻?/strong>�。� 參考：《安徽醫(yī)科大學(xué)學(xué)報》2016年09期
【摘要】：目的探討應(yīng)用依那普利拉的早期干預(yù)對嚴(yán)重?zé)齻笫笮募p害的保護(hù)作用,并對其作用機(jī)制進(jìn)行初步研究。方法 66只雌性清潔級SD大鼠,隨機(jī)將大鼠分為對照組6只、燒傷組30只、治療組30只。燒傷組和治療組分為傷后1、3、6、12、24 h 5個時相點,每時相點6只大鼠。燒傷組與治療組大鼠采用熱水直接燙傷法制作嚴(yán)重?zé)齻Ｐ?傷后予以腹腔注射補(bǔ)液抗休克,治療組加用依那普利拉早期干預(yù),均在傷后各時相點行腹主動脈采血,留取心臟組織,ELISA法檢測血清心肌肌鈣蛋白Ⅰ(cTnⅠ)、白介素1(IL-1)、腫瘤壞死因子α(TNF-α)、血管緊張素Ⅱ(AngⅡ)水平、心肌AngⅡ水平,部分心肌組織應(yīng)用TUNEL法檢測細(xì)胞凋亡情況。結(jié)果燒傷組與治療組血清cTnI水平在傷后各時相點較對照組均有顯著性升高(P0.05),治療組各時相點血清cTnI水平升高幅度均低于燒傷組(P0.05);燒傷組與治療組血清IL-1、TNF-α水平各時相點較對照組均有顯著性升高(P0.05),治療組各時相點血清IL-1、TNF-α水平升高幅度均低于燒傷組(P0.05);燒傷組心肌勻漿及血清中AngⅡ在傷后顯著性升高(P0.05),治療組各時相點血清及心肌勻漿中AngⅡ水平升高幅度均低于燒傷組,其中傷后1、12 h差異有統(tǒng)計學(xué)意義(P0.05);燒傷后心肌細(xì)胞凋亡數(shù)量在各個時相點顯著增加,治療組各個時相點凋亡數(shù)量顯著少于燒傷組(P0.05)。結(jié)論嚴(yán)重?zé)齻笫笤缙诩窗l(fā)生心肌損傷,依那普利拉對燒傷大鼠心肌損傷起保護(hù)作用,其保護(hù)作用機(jī)制可能與減少燒傷后IL-1、TNF-α、AngⅡ的釋放,減少心肌細(xì)胞凋亡有關(guān)。
[Abstract]:Objective to investigate the protective effect of early intervention of enalapril on myocardial injury in severely burned rats and its mechanism. Methods 66 female SD rats of clean grade were randomly divided into control group (n = 6), burn group (n = 30) and treatment group (n = 30). The burn group and the treatment group were divided into 5 time points, 6 rats at each time point. Rats in burn group and treatment group were treated with hot water direct scalding method to make severe burn model. The rats in treatment group were treated with intraperitoneal injection of rehydration to resist shock. The treatment group was treated with early intervention of enalapril, and blood was collected from abdominal aorta at each time point after injury. The serum levels of cardiac troponin 鈪，
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