本文選題：卡巴膽堿 + 膿毒癥��； 參考：《廣東醫(yī)學》2016年16期

【摘要】：目的探討卡巴膽堿對膿毒癥大鼠腸道上皮細胞通透性和緊密連接的作用。方法取健康成年雄性SD大鼠,隨機分為4組(每組8只),經腹腔注射10 mg/kg脂多糖(LPS)復制膿毒癥模型:對照組:腹腔注射等體積的生理鹽水;膿毒癥組:腹腔注射10 mg/kg LPS;卡巴膽堿組:腹腔注射10 mg/kg LPS,15 min后腹腔注射0.1mg/kg卡巴膽堿;α-BGT組:腹腔注射10 mg/kg LPS,5 min后皮下注射1.0μg/kgα-BGT,10 min后腹腔注射0.1 mg/kg卡巴膽堿。給予LPS 3 h后放血處死動物,取回腸組織,光鏡下觀察腸道上皮組織損害,熒光分光光度計測量體外漏出的FITC-Dextran,統(tǒng)計腸系膜淋巴結、肝、脾中細菌移位數(shù)量,電鏡下觀察腸道上皮細胞緊密連接超微結構變化,共聚焦顯微鏡下觀察腸道上皮細胞緊密連接ZO-1和claudin-2蛋白移位,Western blot法檢測緊密連接ZO-1和claudin-2蛋白含量。結果給予LPS后3 h,各組大鼠腸黏膜上皮細胞嚴重損傷,微絨毛壞死、脫落,炎性細胞浸潤伴出血,腸黏膜組織學評分顯著升高(P0.05),腸道上皮組織通透性顯著升高(P0.05),腸系膜淋巴結、肝、脾中的菌落數(shù)明顯增加(P0.05),腸道上皮細胞緊密連接結構破壞,為低密度電子致密影,腸道上皮細胞緊密連接ZO-1和claudin-2蛋白結構缺失,細胞周圍著色減少,大量移位。腹腔注射卡巴膽堿后可顯著減輕膿毒癥大鼠上述改變,皮下注射α-BGT后可逆轉卡巴膽堿的上述作用。結論卡巴膽堿可減輕膿毒癥大鼠腸道上皮細胞損傷,降低其通透性,改善上皮細胞緊密連接蛋白移位和表達,該作用是通過激活膽堿能抗炎通路完成的。
[Abstract]:Objective to investigate the effect of Kaba choline on the permeability and close connection of intestinal epithelial cells in rats with sepsis. Methods the healthy adult male SD rats were randomly divided into 4 groups (8 rats in each group), and 10 mg/kg lipopolysaccharides (LPS) were injected into the abdominal cavity to replicate the sepsis model: the control group was injected into the abdominal cavity with equal volume of saline, and the sepsis group was injected 10 mg/kg intraperitoneally. LPS: Kaba choline group: intraperitoneal injection of 10 mg/kg LPS, 15 min after intraperitoneal injection of 0.1mg/kg Kaba choline; alpha -BGT group: intraperitoneal injection of 10 mg/kg LPS, 5 min after subcutaneous injection of 1 micron g/kg alpha -BGT, 10 min after the abdominal injection of 0.1 Kaba choline. FITC-Dextran was measured in vitro by spectrophotometer. The number of bacterial translocations in mesenteric lymph nodes, liver and spleen were counted. Ultrastructural changes were observed under electron microscopy. Under confocal microscopy, the intestinal epithelial cells were closely linked to ZO-1 and Claudin-2 egg white shift, and ZO-1 and Claudi were closely linked by Western blot method. The content of n-2 protein was 3 h after LPS. The intestinal mucosal epithelial cells were seriously damaged, microvilli necrosis, shedding, inflammatory cell infiltration and bleeding, intestinal mucosa histology score increased significantly (P0.05), intestinal epithelial tissue permeability increased significantly (P0.05), intestinal mesangial lymph node, liver and spleen increased significantly (P0.05), intestinal epithelium The cell closely connected structure was destroyed by the low density electron dense shadow, the intestinal epithelial cells closely connected ZO-1 and Claudin-2 protein structure loss, the peripheral coloring of the cells decreased, and a large amount of displacement. After intraperitoneal injection of Kaba choline, the above changes in septic rats could be significantly reduced, and the above effect of Kaba choline could be reversed after subcutaneous injection of alpha -BGT. Conclusion card Choline alleviates intestinal epithelial cell damage in septic rats, reduces its permeability and improves the displacement and expression of close connexin in epithelial cells, which is accomplished by activating the cholinergic anti-inflammatory pathway.
【作者單位】： 鄭州大學第五附屬醫(yī)院消化內科;河南省人民醫(yī)院消化科;
【分類號】：R459.7

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