本文選題：急性壞死性胰腺炎 + 內(nèi)臟大神經(jīng)��； 參考：《河南科技大學(xué)》2013年碩士論文

【摘要】：目的：探討切斷內(nèi)臟交感神經(jīng)對(duì)早期犬急性壞死性胰腺炎（ANP）病理生理學(xué)的影響及探索治療ANP新思路。 方法：取20只健康雜種犬隨機(jī)分為假手術(shù)組（n=4）；ANP模型組8只（模型組，n=8）；ANP模型+切斷雙側(cè)內(nèi)臟大神經(jīng)組（實(shí)驗(yàn)組，n=8）。動(dòng)態(tài)監(jiān)測(cè)各組外周血清的血淀粉酶、超敏C-反應(yīng)蛋白、血清鈣、腫瘤壞死因子-α、白介素-10的改變。7天后處死全部動(dòng)物并做病理觀察。結(jié)果：1.血淀粉酶：假手術(shù)組顯著低于模型組、實(shí)驗(yàn)組，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；模型組與實(shí)驗(yàn)組比較，差異無(wú)統(tǒng)計(jì)學(xué)意義（P＞0.05）。2.超敏C-反應(yīng)蛋白：三組血清超敏C-反應(yīng)蛋白水平模型復(fù)制成功后均升高，術(shù)后1天達(dá)到峰值，，假手術(shù)組升高水平較低，達(dá)到峰值后下降快；模型組升高水平最高，達(dá)到峰值后下降最慢；實(shí)驗(yàn)組升高水平高，達(dá)到峰值后下降慢。假手術(shù)組與模型組、實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；模型組與實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）。3.血鈣水平：三組血鈣水平模型復(fù)制成功后均降低，假手術(shù)組血清鈣水平復(fù)制成功后降低不明顯，基本在正常范圍內(nèi)；模型組血清鈣水平復(fù)制成功后顯著降低，有明顯的低血鈣癥狀；實(shí)驗(yàn)組血清鈣水平復(fù)制成功后降低，有低血鈣癥狀。假手術(shù)組與模型、實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；模型組與實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）。4.腫瘤壞死因子-α：三組血清腫瘤壞死因子-α水平模型復(fù)制成功后升高，術(shù)后1天高到到峰值，假手術(shù)組升高水平較低，達(dá)到峰值后下降快；模型組升高水平最高，達(dá)到峰值后下降速度最慢；實(shí)驗(yàn)組升高水平中等，達(dá)到峰值后下降速度較慢。假手術(shù)組與模型、實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；模型組與實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）。5.白介素-10：三組血清白介素-10模型復(fù)制成功后均升高，術(shù)后1天升高到到峰值，假手術(shù)組升高水平較低，達(dá)到峰值后下降快；模型組升高水平高，達(dá)到峰值后下降慢；實(shí)驗(yàn)組升高水平最高，達(dá)到峰值后下降速度最慢。假手術(shù)組與模型、實(shí)驗(yàn)組比較，差異有統(tǒng)計(jì)學(xué)意義（P＜0.01）；型組與實(shí)驗(yàn)組比較差異有統(tǒng)計(jì)學(xué)意義（P＜0.05）。6.病理評(píng)分：B、C組較A組高，B組較C組高，差異有統(tǒng)計(jì)學(xué)意義（p＜0.05）。 結(jié)論：1.切斷內(nèi)臟交感神經(jīng)能改善ANP犬早期嚴(yán)重的應(yīng)激反應(yīng)及過(guò)度的炎癥反應(yīng)狀態(tài)。2.切斷內(nèi)臟交感神經(jīng)能延緩ANP犬早期病理生理學(xué)變化進(jìn)程，且能減輕ANP犬的胰腺病理?yè)p傷。
[Abstract]:Objective: to investigate the effect of transection of visceral sympathetic nerve on the pathophysiology of early acute necrotizing pancreatitis (ANP) in dogs and to explore a new idea for the treatment of ANP. Methods: twenty healthy mongrel dogs were randomly divided into sham operation group (n = 8) and model group (n = 8). The changes of serum amylase, hypersensitive C-reactive protein, serum calcium, tumor necrosis factor- 偽 and interleukin-10 in peripheral blood of each group were dynamically monitored. The result is 1: 1. Serum amylase level in sham-operated group was significantly lower than that in model group (P < 0.01), but there was no significant difference between model group and experimental group (P > 0.05). Hypersensitive C- reactive protein: the serum hypersensitive C- reactive protein level of the three groups increased after replicating successfully, reached the peak on the first day after operation, and decreased quickly after reaching the peak in the sham-operation group, and the elevation level in the model group was the highest. In the experimental group, the rising level was high, and the decrease was slow after reaching the peak value. There was significant difference between the sham operation group and the model group (P < 0.01), and the difference between the model group and the experimental group was statistically significant (P < 0.05) and the difference between the model group and the experimental group was significant (P < 0.05). Serum calcium level: the serum calcium level of the three groups decreased after the successful replication, but the serum calcium level of the sham operation group was not significantly decreased after the successful replication, basically within the normal range, the serum calcium level of the model group decreased significantly after the successful replication, and the serum calcium level of the model group decreased significantly after the successful replication of the model group. There were obvious hypocalcemia symptoms, and the serum calcium levels in the experimental group decreased after successful replication, and there were hypocalcemia symptoms. There was significant difference between the sham operation group and the model group (P < 0.01), and the difference between the model group and the experimental group was statistically significant (P < 0.05). Tumor necrosis factor- 偽: the serum tumor necrosis factor- 偽 level in the three groups increased after replicating successfully, reached the peak on the 1st day after operation, and decreased rapidly in the sham operation group, the highest level in the model group, and the highest in the model group. The descending speed was the slowest after the peak value, while the rising level was moderate in the experimental group, and the decreasing rate was slower after the peak value. There was significant difference between the sham operation group and the model group (P < 0.01), and the difference between the model group and the experimental group was statistically significant (P < 0.05). Interleukin-10: the serum interleukin-10 (IL-10) level in the three groups increased to its peak one day after replication and reached the peak level in the sham-operation group, and decreased quickly after the peak in the sham-operation group, the elevation level in the model group was higher than that in the control group, and the decrease was slow after reaching the peak value. The experimental group had the highest level of elevation and the slowest descent rate after reaching the peak value. There was significant difference between the sham operation group and the model group (P < 0.01), and between the type group and the experimental group (P < 0.05). The pathological score of group C was higher than that of group A and group B was higher than that of group C, and the difference was statistically significant (P < 0.05). Conclusion 1. Transection of splanchnic sympathetic nerve can improve early severe stress response and excessive inflammatory response in ANP dogs. Transection of visceral sympathetic nerve could delay the pathophysiological changes in ANP dogs and alleviate the pancreatic pathological injury in ANP dogs.
【學(xué)位授予單位】：河南科技大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類(lèi)號(hào)】：R657.51

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