本文選題：hepcidin + 膿毒血癥�。� 參考：《浙江大學》2014年碩士論文

【摘要】：目的： 探討hepcidin基因干擾對膿毒癥小鼠淋巴細胞凋亡的影響,為膿毒癥的發(fā)病機制和防治提供一定理論參考。 方法： BALB/C雄性6-8周健康小鼠10只,隨機均分為實驗組(hepcidin基因干擾組,n=5)和對照組(RNA干擾腺病毒組,n=5)。實驗組小鼠給予尾靜脈高動力注射hepcidin特異性siRNA (small interference RNA)腺病毒,對照組小鼠給予尾靜脈高動力注射對照的RNA干擾腺病毒,兩組小鼠均自由飲食和活動。13天后,兩組小鼠采用盲腸結(jié)扎穿孔(cecal ligation and puncture, CLP)法復(fù)制膿毒癥模型。CLP模型制備24小時后處死小鼠,留取全血和脾臟標本。采用流式細胞術(shù)測定脾臟T淋巴細胞亞型CD4+和CD8+的數(shù)量百分比；TUNEL法檢測脾臟組織細胞的凋亡量；Annexin-V染色流式檢測外周血和脾臟T淋巴細胞的凋亡百分比；Western blot法檢測脾臟組織凋亡相關(guān)蛋白caspase-3和caspase-9的活化情況。 結(jié)果： 實驗組小鼠脾臟CD4+.T細胞百分比明顯低于對照組(17.34%vs.29.08%,P0.05)；實驗組小鼠脾臟CD8--T細胞百分比明顯低于對照組(8.37%vs.14.57%,P0.05); TUNEL結(jié)果顯示實驗組小鼠脾臟組織細胞凋亡量比對照組小鼠多；實驗組小鼠脾臟T淋巴細胞凋亡率較對照組明顯增多(79.3%vs.59.6%,P0.05),實驗組小鼠外周血T淋巴細胞凋亡率較對照組明顯增加(93.0%vs.68.9%,P0.05)；實驗組小鼠脾臟組織凋亡相關(guān)蛋白caspase-3和caspase-9活化水平較對照組明顯增強(11.97vs.1.54, P0.05;2.22vs.1.09, P0.05)。 結(jié)論： Hepcidin基因干擾引起膿毒癥小鼠淋巴細胞凋亡增加,hepcidin可能在膿毒癥中對淋巴細胞的凋亡起一定的抑制作用。
[Abstract]:Objective: To investigate the effect of hepcidin gene interference on lymphocyte apoptosis in septic mice, and to provide a theoretical reference for the pathogenesis, prevention and treatment of sepsis. Methods: Ten healthy BALB/C male mice were randomly divided into two groups: the experimental group was treated with hepcidin gene interference and the control group with adenovirus. The mice in the experimental group were injected with hepcidin specific siRNA small interference adenovirus, and the control mice were given RNA interference adenovirus. The mice in both groups were fed free diet and activity for 13 days. The sepsis model was made by cecal ligation and puncture (CLP) method in the two groups. The mice were killed 24 hours later, and the whole blood and spleen samples were collected. Flow cytometry was used to determine the percentage of CD4 and CD8 in spleen T lymphocyte subtypes and Tunel method was used to detect apoptosis of splenic tissue cells. Annexin-V staining flow cytometry was used to detect the percentage of apoptosis in peripheral blood and spleen T lymphocytes. Western blot method was used to detect the percentage of apoptosis in peripheral blood and spleen T lymphocytes. Activation of apoptosis-related proteins caspase-3 and caspase-9 in spleen tissue. Results: The percentage of CD4. T cells in the spleen of the experimental group was significantly lower than that of the control group (17. 34 vs 29. 08), the percentage of the spleen CD8--T cells in the experimental group was significantly lower than that in the control group (8. 37 vs 14. 57). The results of TUNEL showed that the apoptosis of spleen tissue cells in the experimental group was more than that in the control group. Compared with the control group, the apoptosis rate of spleen T lymphocytes in the experimental group was significantly higher than that in the control group. The apoptosis rate of T lymphocytes in the peripheral blood of the experimental group was significantly higher than that of the control group, and the apoptosis-related protein caspase-3 and caspase-9 activated water in the spleen of the experimental group were significantly higher than that of the control group. Compared with the control group, P0.05 + 2.22vs.1.09, P0.05 + 2.22vs.1.09. Conclusion: Apoptosis of lymphocytes in septic mice induced by Hepcidin gene interference may be inhibited by hepcidin in sepsis.
【學位授予單位】：浙江大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R459.7

【參考文獻】

相關(guān)期刊論文 前1條

1 立彥;李秀華;瞿衛(wèi);陳永銘;洪亮;鄭署云;;感染性休克患者外周血T淋巴細胞凋亡和影響因素分析[J];臨床薈萃;2011年18期

，

本文編號：1853561