本文選題：迷走神經(jīng)電刺激 + 腦外傷　； 參考：《南昌大學(xué)》2017年碩士論文

【摘要】：目的:探討迷走神經(jīng)電刺激(vugal nerve stimulation,VNS)對(duì)TBI后昏迷大鼠的促醒作用及其對(duì)大鼠前額葉皮層N-甲基-D-天冬氨酸(N-methyl-D-aspartate,NMDA)受體亞基NR1表達(dá)的影響,并闡明Orexin-A在其中所起的在作用。方法:將SD大鼠隨機(jī)分為4組(每組36只):空白對(duì)照組(Control)、模型組(TBI)、刺激組(TBI+VNS)和orexin-1受體(OXlR)拮抗劑組(TBI+SB334867+VNS)。大鼠經(jīng)“自由落體法”建立TBI昏迷模型后進(jìn)行VNS治療。根據(jù)各組大鼠的行為學(xué)變化進(jìn)行意識(shí)分級(jí),并于VNS后的6、12、24小時(shí)每組分別處死12只大鼠,取其前額葉皮層組織,應(yīng)用免疫組化、Western-blot技術(shù)檢測(cè)NR1表達(dá)的變化。結(jié)果:刺激組有23只蘇醒,而模型組中僅蘇醒11只,與此同時(shí),刺激組大鼠前額葉皮層NR1的表達(dá)水平要明顯高于模型組(P0.05)。OX1R拮抗劑組蘇醒的大鼠(16只)少于刺激組,且NR1的表達(dá)也較刺激組顯著降低(P0.05)。另外,模型組大鼠NR1的表達(dá)水平較空白對(duì)照組也顯著降低(P0.05)。組內(nèi)比較的結(jié)果顯示,NR1的表達(dá)水平在模型組中6、12、24小時(shí)呈現(xiàn)逐漸上升的趨勢(shì)(P0.05),而在刺激組中的三個(gè)時(shí)間點(diǎn)呈現(xiàn)依次遞減的趨勢(shì)(P0.05)。結(jié)論:迷走神經(jīng)電刺激有助于腦外傷后昏迷大鼠意識(shí)狀態(tài)的恢復(fù),且能夠上調(diào)大鼠前額葉皮層NR1的表達(dá)。此外,VNS促醒的作用機(jī)制可能與Orexin-A對(duì)NR1表達(dá)的調(diào)控有關(guān)。
[Abstract]:Aim: to investigate the effects of vagal nerve stimulation (VNSs) on the prowakening of coma rats after TBI and on the expression of N- methyl-Daspartate N-methyl-D-aspartate receptor subunit (NR1) in the prefrontal cortex of rats, and to elucidate the role of Orexin-A in the expression of N-methyl-Daspartate receptor subunit (NMDAR) in the prefrontal cortex of rats. Methods: SD rats were randomly divided into 4 groups (36 rats in each group: control group, model group, stimulation group) and orexin-1 receptor OXlR antagonist group. TBI coma model was established by free fall method in rats and then treated with VNS. According to the behavioral changes of rats in each group, the consciousness was graded, and 12 rats were killed in each group at 612h 24 hours after VNS. The prefrontal cortex tissues were taken and the expression of NR1 was detected by immunohistochemistry Western-blot technique. Results: 23 rats were awake in the stimulation group, but only 11 in the model group. At the same time, the expression level of NR1 in the prefrontal cortex in the stimulation group was significantly higher than that in the model group (P 0.05) .OX1R antagonist group (16 rats) was less than the stimulation group. The expression of NR1 was significantly lower than that of stimulation group (P 0.05). In addition, the expression of NR1 in the model group was significantly lower than that in the control group. The results of comparison within the group showed that the expression of NR1 in the model group showed a gradual upward trend in 24 hours, while the three time points in the stimulation group showed a decreasing trend of P0.05 in turn. Conclusion: vagal electrical stimulation is helpful to the recovery of conscious state and up-regulates the expression of NR1 in prefrontal cortex of rats after brain injury. In addition, the mechanism of VNS may be related to the regulation of NR1 expression by Orexin-A.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R651.15

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