本文選題：熱打擊　切入點(diǎn)：細(xì)胞凋亡　出處：《解放軍醫(yī)學(xué)雜志》2017年01期

【摘要】：目的觀察熱打擊對(duì)大鼠腸上皮細(xì)胞(IEC-6)氧化應(yīng)激反應(yīng)、溶酶體損傷及細(xì)胞凋亡的影響,探討中暑熱損傷過程中腸道損傷的機(jī)制。方法將IEC-6細(xì)胞分為對(duì)照組(細(xì)胞僅置于37℃、5%CO2細(xì)胞培養(yǎng)箱中孵育)、熱打擊組(細(xì)胞置于43℃、5%CO2細(xì)胞培養(yǎng)箱中1h,之后分別置于正常細(xì)胞培養(yǎng)箱中繼續(xù)孵育0、1、3、6、12h)、藥物干預(yù)組(于熱打擊前分別采用溶酶體抑制劑E-64和活性氧清除劑NAC預(yù)處理細(xì)胞1h)。采用DCFH-DA法檢測(cè)細(xì)胞內(nèi)活性氧(ROS)的表達(dá),吖啶橙(AO)染色法檢測(cè)溶酶體膜穩(wěn)定性,AnnexinⅤFITC/PI雙染色法檢測(cè)細(xì)胞凋亡率,CCK-8法檢測(cè)細(xì)胞活力。結(jié)果與對(duì)照組比較,熱打擊后復(fù)溫1h即導(dǎo)致IEC-6細(xì)胞活力下降,凋亡增加,且在復(fù)溫后12h時(shí)最為明顯(P0.05);熱打擊后即刻即引起細(xì)胞內(nèi)ROS表達(dá)及"蒼白細(xì)胞"數(shù)量增加,且在復(fù)溫后12h時(shí)最為明顯(P0.05)。與熱打擊組比校,采用NAC對(duì)細(xì)胞進(jìn)行預(yù)處理可明顯提高熱打擊后細(xì)胞活力,減輕溶酶體損傷,并降低細(xì)胞凋亡率(P0.05);采用E-64對(duì)細(xì)胞進(jìn)行預(yù)處理可明顯提高熱打擊后細(xì)胞活力,并降低細(xì)胞凋亡率(P0.05)。結(jié)論 ROS介導(dǎo)了熱打擊誘導(dǎo)腸上皮細(xì)胞凋亡的調(diào)控,這可能是中暑導(dǎo)致腸道黏膜屏障功能損傷的重要機(jī)制之一。
[Abstract]:Objective to observe the effects of heat shock on oxidative stress, lysosome injury and apoptosis in rat intestinal epithelial cells (IEC-6), and to explore the mechanism of intestinal injury during heat injury.Methods IEC-6 cells were divided into control group (the cells were incubated in 37 鈩，

本文編號(hào)：1703286