本文選題：肝素　切入點(diǎn)：脫細(xì)胞真皮基質(zhì)　出處：《內(nèi)蒙古大學(xué)》2013年碩士論文

【摘要】：目的1.制作肝素化選擇性羊脫細(xì)胞真皮基質(zhì)(acelluar dermal matrix, ADM)。2.運(yùn)用肝素化羊ADM治療大鼠深二度燙傷,研究燙傷創(chuàng)面愈合率和創(chuàng)面組織結(jié)構(gòu)的變化情況。3.探討肝素化羊ADM治療燒傷創(chuàng)面時(shí),創(chuàng)面組織中細(xì)胞凋亡的變化情況,從而揭示該生物敷料在促進(jìn)創(chuàng)面愈合的一些機(jī)制。方法1.選擇1歲齡健康綿羊,取斷層皮片制作選擇性羊ADM。2.160只健康Wistar大鼠制作成深二度燙傷模型,隨機(jī)分為碘伏紗布治療組、豬ADM治療組、羊ADM治療組和肝素化羊ADM治療組。3.燙傷大鼠在術(shù)3d、7d、14d、21d和28d計(jì)算大鼠創(chuàng)面愈合率。并同時(shí)在各個(gè)時(shí)間段處死大鼠,取創(chuàng)面組織標(biāo)本做HE染色和TUNEL原位細(xì)胞凋亡染色,觀察創(chuàng)面愈合不同時(shí)期內(nèi)組織結(jié)構(gòu)變化情況。結(jié)果1.成功制作選擇性羊ADM,表皮完整,真皮未見(jiàn)細(xì)胞成分、血管及皮膚附件。2.燙傷后14d、21d、28d時(shí),豬ADM組、羊ADM組和肝素化羊ADM組的創(chuàng)面愈合率均優(yōu)于碘伏紗布組(P0.05),21d肝素化羊ADM組優(yōu)于豬ADM組(P0.05),21d和28d時(shí)肝素化羊ADM組優(yōu)于羊ADM組(P0.05)。3.各組創(chuàng)面HE染色顯示,傷后21d,豬ADM組和羊ADM組真皮層膠原纖維排列欠規(guī)整,成纖維細(xì)胞開(kāi)始出現(xiàn)較多的增長(zhǎng)。而肝素化羊ADM組傷后21天時(shí)表皮、真皮結(jié)構(gòu)基本正常,膠原纖維排列比較規(guī)則。4.各組深二度在燙傷后第7d,各組創(chuàng)面組織中成纖維細(xì)胞的凋亡較3d時(shí)均有明顯的增加,但各組間無(wú)明顯的統(tǒng)計(jì)差異(P0.05)。隨著大鼠創(chuàng)面的愈合,在燙傷后14d-28d,各組創(chuàng)面組織中的成纖維細(xì)胞凋亡數(shù)有明顯的增加,豬ADM組、羊ADM組和肝素化羊ADM組中的細(xì)胞凋亡數(shù)均較碘伏紗布組有明顯的增加(P0.05),其中尤以肝素化羊ADM治療組在術(shù)后28d的數(shù)字最高(P0.05)。結(jié)論1.成功制作了選擇性羊皮真皮基質(zhì)。2.三種真皮基質(zhì)治療深二度燒傷創(chuàng)面,其中尤以肝素化羊ADM在促進(jìn)創(chuàng)面愈合組織結(jié)構(gòu)恢復(fù)方面的效果更好。3.肝素化羊ADM在燒傷創(chuàng)面愈合后期,較其它組能更加的誘導(dǎo)或促進(jìn)創(chuàng)面肉芽組織中成纖維細(xì)胞的細(xì)胞凋亡,減少瘢痕的形成,從而促進(jìn)創(chuàng)面修復(fù)愈合。
[Abstract]:Objective 1. To make heparinized selective acelluar dermal matrix, ADM.2.Heparinized sheep ADM was used to treat deep second degree scalding in rats. The healing rate and tissue structure of burn wounds were studied. 3. To investigate the effect of heparin-treated sheep ADM on burn wounds. The changes of cell apoptosis in wound tissue, so as to reveal the mechanism of the biological dressing in promoting wound healing. 1. Choose 1 year old healthy sheep. The model of deep second degree scalding was made from selective sheep ADM.2.160 rats by skin grafts. The rats were randomly divided into two groups: iodophor gauze treatment group and porcine ADM treatment group. Sheep ADM group and heparinized sheep ADM group .3.The wound healing rate of scalded rats was calculated on the 3rd day, 7d, 14d, 21d and 28d, and the rats were killed at the same time. The wound tissue samples were stained by HE and TUNEL in situ apoptosis staining. The changes of tissue structure in different periods of wound healing were observed. 1. Selective sheep ADM was made successfully. The epidermis was intact, no cell components were found in dermis, blood vessels and skin appendages were found. At 14 days after burn, 21 days after scald, pig ADM group was treated. The wound healing rate of sheep ADM group and heparin treated sheep ADM group was better than that of iodophor gauze group on 21d after heparin treatment, and that of heparin treated sheep ADM group was better than that of porcine ADM group at 21d and 28d. The wound surface of heparin treated sheep ADM group was superior to that of ADM group (P 0.05N. 3). On the 21st day after injury, the collagenous fibers in the dermis of pig ADM group and sheep ADM group were irregular, and fibroblasts began to increase, while the epidermis and dermis structure of heparinized sheep ADM group were basically normal at 21 days after injury. On the 7th day after scalding, the apoptosis of fibroblasts in the wound tissue of each group increased significantly, but there was no significant statistical difference between the groups (P 0.05). With the healing of the wound in rats, the apoptosis of fibroblasts in the wound tissue of each group was significantly increased than that of the third day after scald, but there was no significant statistical difference between the groups. The number of fibroblast apoptosis in wound tissue of each group increased significantly at 14 d to 28 d after scalding, and the number of fibroblasts in pig ADM group was significantly increased. The number of apoptosis in sheep ADM group and heparinized sheep ADM group was significantly higher than that in iodophor gauze group, especially in heparinized sheep ADM group. Conclusion 1. Selective dermal matrix of sheep skin was successfully made. Treatment of deep second degree burn wounds with three kinds of dermal matrix, Especially heparinized sheep ADM was more effective in promoting the recovery of wound healing tissue structure. Heparin sheep ADM could induce or promote the apoptosis of fibroblasts in granulation tissue more than other groups in the late stage of burn wound healing. Reduce scar formation, thereby promoting wound healing.
【學(xué)位授予單位】：內(nèi)蒙古大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R644

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張曉暉,姚天明,黃高f,王文亮;細(xì)胞凋亡的最新研究進(jìn)展[J];第四軍醫(yī)大學(xué)學(xué)報(bào);2002年S1期

2 劉潮波;王偉琴;程懷正;;微粒皮在修復(fù)領(lǐng)域中的應(yīng)用[J];河北醫(yī)學(xué);2006年04期

3 付春景,郭龍輝,申琦,黃幼田,許燕;心肌肥大和心力衰竭大鼠心肌細(xì)胞凋亡及Bax和Bcl-2的表達(dá)[J];鄭州大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2005年03期

4 谷慶陽(yáng),曹衛(wèi)紅,王德文,高亞兵,楊志祥,趙坡;P53,Bax,Bcl-2蛋白表達(dá)及細(xì)胞凋亡在急性放射性皮膚潰瘍發(fā)生發(fā)展過(guò)程中的作用探討[J];軍事醫(yī)學(xué)科學(xué)院院刊;2001年02期

5 呂凱歌;張麗紅;裴慶國(guó);潘可風(fēng);蔣欣泉;張秀麗;;多孔支架材料PLGA/HA生物相容性的實(shí)驗(yàn)研究[J];口腔頜面外科雜志;2007年02期

6 苑麗霞;孫毅;楊艷君;趙鴻飛;;細(xì)胞程序性死亡機(jī)制的研究[J];山西農(nóng)業(yè)科學(xué);2008年08期

7 呂大倫,王云龍,林軍,侯英勇,劉雯蓓,杭守云;LMWH對(duì)燒傷創(chuàng)面微循環(huán)影響的實(shí)驗(yàn)研究[J];皖南醫(yī)學(xué)院學(xué)報(bào);2001年03期

8 姜篤銀,陳璧,賈赤宇,陶克;異種脫細(xì)胞真皮基質(zhì)抗原性的實(shí)驗(yàn)研究[J];中華燒傷雜志;2003年03期

9 陳璧;深Ⅱ度燒傷創(chuàng)面治療與愈合后的關(guān)系[J];中華燒傷雜志;2005年01期

10 劉強(qiáng);孟愛(ài)華;賀立新;楊紅明;柴家科;;豬脫細(xì)胞真皮基質(zhì)在體內(nèi)轉(zhuǎn)歸的動(dòng)物實(shí)驗(yàn)與臨床觀察[J];中華燒傷雜志;2006年05期

，

本文編號(hào)：1676045