本文選題：高壓電　切入點(diǎn)：燒傷　出處：《河北醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:高壓電燒傷是一種特殊原因所致的損傷,燒傷后體內(nèi)氧自由基產(chǎn)生增多和(或)機(jī)體抗氧化能力下降所引起的脂質(zhì)過(guò)氧化、蛋白氧化、DNA損傷而造成嚴(yán)重的細(xì)胞、組織、器官損害,血清中谷胱甘肽(glutathione,GSH)含量變化是減輕機(jī)體氧化應(yīng)激損傷的關(guān)鍵因素之一。在還原反應(yīng)中,谷胱甘肽過(guò)氧化物酶(Glutathione peroxidase,GPx)通過(guò)將GSH作為電子供體使H2O2解毒,并生成終產(chǎn)物氧化型谷胱甘肽(oxidized glutathione,GSSG)。谷胱甘肽還原酶(glutathione reductase,GR)是將GSSG還原為GSH的催化酶。因此,我們?cè)O(shè)計(jì)本實(shí)驗(yàn),旨在研究高壓電燒傷早期大鼠血清GSSG、GR變化,并通過(guò)烏司他丁(ulinastatin,UTI)進(jìn)行干預(yù),研究燒傷后GSSG、GR的變化機(jī)制,探討UTI治療高壓電燒傷的可行性。方法:1實(shí)驗(yàn)動(dòng)物分組:健康成年SD大鼠(由河北醫(yī)科大學(xué)動(dòng)物實(shí)驗(yàn)中心提供,體重291~362g,動(dòng)物許可證號(hào):SCXK(冀)2013-1-003,合格證編號(hào)832457)240只,按隨機(jī)數(shù)字表法分成四組,假高壓電燒傷組(簡(jiǎn)稱:假傷組)、高壓電燒傷組(簡(jiǎn)稱:電傷組)、高壓電燒傷UTI治療組(簡(jiǎn)稱:UTI組)、高壓電燒傷鹽水治療組(簡(jiǎn)稱:鹽水組),每組各60只。每組又按觀察時(shí)間分為電擊前15min、電擊后5min、電擊后1h、2h、4h、8h六個(gè)時(shí)相組,每時(shí)相組10只。2實(shí)驗(yàn)前準(zhǔn)備:將大鼠編號(hào)、稱重,左上肢、右下肢及前胸脫毛。將實(shí)驗(yàn)藥品按實(shí)驗(yàn)需要配成所需濃度。3高壓電燒傷模型制作:首先連接好實(shí)驗(yàn)變壓器和調(diào)壓器電線。用10%水合氯醛溶液腹腔注射麻醉大鼠,按40mg/kg給藥,麻醉成功后,將大鼠仰臥于專用電擊實(shí)驗(yàn)臺(tái)上,固定四肢,將兩個(gè)1cm×1cm電極片分別固定于大鼠的左上肢(電流入口)、右下肢(電流出口)脫毛區(qū)。接通電源后,調(diào)整調(diào)壓器使升壓器輸出電壓至2k V,連接升壓器,使高壓電流通過(guò)大鼠,電擊3s。對(duì)照組制作假電傷模型,不合閘通過(guò)電流,其余步驟與電傷組一致。電擊5min內(nèi),UTI組腹腔注射2×104U/kg UTI,鹽水組腹腔內(nèi)注射等量的生理鹽水。4樣本采集與保存:將高壓電燒傷模型復(fù)制成功的大鼠開(kāi)胸暴露心臟,直視下心臟抽血6ml,置于一次性使用真空采血管(紅)中,輕輕顛倒數(shù)次后靜置30min,上離心機(jī),以3000轉(zhuǎn)/min離心10min,紅管中取上清液置于Eppendorf管中在-70℃條件下保存。5指標(biāo)檢測(cè):置于冰箱的血清采用ELISA雙夾心抗體法,檢測(cè)GSSG、GR含量。6數(shù)據(jù)處理:采用SPSS 21.0統(tǒng)計(jì)軟件,行兩因素析因設(shè)計(jì)的方差分析。以P0.05為顯著性檢驗(yàn)水準(zhǔn)。結(jié)果:1大鼠血清GSSG含量變化電傷組GSSG含量與假傷組相比明顯升高(P0.05),且GSSG含量電傷后在一定時(shí)間內(nèi)有顯著差異(P0.05),呈上升趨勢(shì)。UTI組GSSG含量總體低于鹽水組(P0.05),且GSSG含量治療后在一定時(shí)間內(nèi)有顯著差異(P0.05),傷后5min、1h、2h、4h、8h與傷前相比有較明顯明顯差異(P0.05)2大鼠血清GR含量變化電傷組GR含量總體高于假傷組(P0.05),且GR含量受傷后隨時(shí)間有顯著差異(P0.05),呈增高趨勢(shì)。UTI組GR含量總體低于鹽水組(P0.05),且GR含量治療后隨時(shí)間有顯著差異(P0.05),UTI組傷后5min~8h各時(shí)相均高于本組傷前值(P0.05)結(jié)論:1高壓電燒傷早期大鼠血清GSSG、GR含量均有不同程度升高,證明燒傷后存在的GSH大量消耗生成GSSG,同時(shí)GR含量升高還原GSSG為GSH以補(bǔ)充機(jī)體所需。2高壓電燒傷UTI組GSSG、GR含量較鹽水組降低,提示UTI治療具有抗氧化作用,能減少GSH轉(zhuǎn)化為GSSG,同時(shí)降低了GR的活性,減輕機(jī)體氧化應(yīng)激損傷。
[Abstract]:Objective : High - voltage burn is a kind of injury caused by special causes . The changes of lipid peroxidation , protein oxidation and DNA damage caused by the increase of oxygen free radicals in the body and the decrease of antioxidant ability of the organism are one of the key factors to alleviate the oxidative stress injury . In the reduction reaction , glutathione peroxidase ( GPx ) is used as electron donor to detoxicate H _ 2O _ 2 , and the final product oxidized glutathione ( oxidized glutathione ) is produced . Methods : We designed this experiment to study the changes of Gssg and GR in rats with high piezoelectric burn . It was divided into four groups according to the random number table method . The rats were divided into four groups according to the random number table method . The rats were divided into four groups according to the random number table method . The results showed that the content of GR in UTI group was significantly higher than that in saline group ( P0.05 ) . The content of GR in UTI group was significantly higher than that in saline group ( P0.05 ) . and the activity of GR is reduced , and the oxidative stress injury of the organism is reduced .

【學(xué)位授予單位】：河北醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R647

【參考文獻(xiàn)】

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本文編號(hào)：1668350