發(fā)布時(shí)間：2018-03-24 18:27

  本文選題：創(chuàng)面愈合　切入點(diǎn)：非線性光譜成像技術(shù)　出處：《福建醫(yī)科大學(xué)》2013年碩士論文

【摘要】：目的：在本研究中，我們運(yùn)用基于雙光子激發(fā)熒光（TPEF）和二次諧波產(chǎn)生（SHG）的非線性光譜成像技術(shù)對創(chuàng)傷愈合過程中膠原蛋白等物質(zhì)進(jìn)行持續(xù)監(jiān)測，從而初步明確其在創(chuàng)面愈合過程中的動態(tài)變化規(guī)律，指導(dǎo)臨床對創(chuàng)傷愈合過程的干預(yù)。方法：選用雄性SD大鼠90只，建立大鼠背部脊柱兩側(cè)圓形皮膚全層切除創(chuàng)傷模型，脊柱右側(cè)創(chuàng)面用“外用重組牛堿性成纖維細(xì)胞生長因子”（Recombinant Bovine Basic Fibroblast Growth Factor For External Use貝復(fù)濟(jì)）進(jìn)行干預(yù)，設(shè)為實(shí)驗(yàn)組；脊柱左側(cè)創(chuàng)面應(yīng)用生理鹽水，設(shè)為對照組，進(jìn)行局部用藥，同體對照，記錄創(chuàng)面愈合時(shí)間，測量創(chuàng)面面積并計(jì)算創(chuàng)面愈合率。分別于造模后的1、2、3、5、7、10、14、20d，隨機(jī)選取10只作為活體掃描標(biāo)本，利用雙光子顯微鏡成像系統(tǒng)活體動態(tài)監(jiān)測創(chuàng)面膠原蛋白的SHG成像信息，結(jié)果：實(shí)驗(yàn)組與對照組在造模后17天時(shí)的創(chuàng)面愈合率分別為100%和95.32%，實(shí)驗(yàn)組創(chuàng)面愈合時(shí)間明顯縮短。實(shí)驗(yàn)及數(shù)據(jù)分析結(jié)果清晰的顯示了大鼠皮膚創(chuàng)面愈合過程中膠原蛋白等物質(zhì)的動態(tài)變化，在創(chuàng)面愈合過程中，膠原蛋白呈明顯的向心性生長。同一時(shí)間，實(shí)驗(yàn)組較對照組膠原蛋白的合成分泌有顯著增多，，統(tǒng)計(jì)學(xué)差異明顯。結(jié)論：非線性光譜成像技術(shù)是基于雙光子激發(fā)熒光（Two-PhotonExcitation Fluorescence TPEF）和二次諧波產(chǎn)生（Second Harmonic GenerationSHG）的生物醫(yī)學(xué)光學(xué)領(lǐng)域的新技術(shù)，以其簡單、無創(chuàng)且可在體成像等特點(diǎn)，在將來有望成為繼病理切片之后的另一種組織形態(tài)學(xué)研究乃至臨床診斷的利器。隨著科技進(jìn)步及光譜成像設(shè)備的小型化發(fā)展，非線性光譜成像技術(shù)在基礎(chǔ)研究與臨床應(yīng)用中擁有巨大的發(fā)展?jié)摿皬V闊的應(yīng)用前景。
[Abstract]:Objective: in this study, we used nonlinear spectral imaging based on two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) to continuously monitor collagen and other substances during wound healing. Therefore, the dynamic changes of the wound healing process were preliminarily clarified, and the clinical intervention on wound healing was guided. Methods: 90 male SD rats were selected to establish the model of full-thickness skin excision on both sides of the dorsal spine of rats. The right spine wound was treated with "recombinant Bovine Basic Fibroblast Growth Factor For External Use" as experimental group, and the left spine wound was treated with normal saline as control group. In the same body control, the wound healing time was recorded, the wound area was measured and the wound healing rate was calculated. Two-photon microscope imaging system was used to monitor the SHG imaging information of collagen in vivo. Results: the wound healing rates of the experimental group and the control group were 100% and 95.32, respectively, and the healing time of the experimental group was significantly shortened. The results of experiment and data analysis clearly showed that the glue was used in the healing process of the rat skin wound. The dynamic changes of proteins and other substances, At the same time, the synthesis and secretion of collagen in the experimental group was significantly higher than that in the control group. Conclusion: nonlinear spectral imaging is a new technique in biomedical optics based on Two-Photon Excitation Fluorescence (TPEF) and second harmonic generation (second Harmonic Generation SHG), which is simple, noninvasive and in vivo imaging. In the future, it is expected to be another kind of sharp weapon for histomorphology research and clinical diagnosis after pathological section. With the development of science and technology and the miniaturization of spectral imaging equipment, Nonlinear spectral imaging technology has great development potential and broad application prospect in basic research and clinical application.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2013
【分類號】：R641

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