本文選題：Orexin-A　切入點(diǎn)：OX1R　出處：《南昌大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討迷走神經(jīng)電刺激(Vagus nerve stimulation,VNS)對腦外傷(Traumatic brain injury,TBI)昏迷大鼠的促醒效果及Orexins在上述效應(yīng)中的調(diào)節(jié)機(jī)制。方法:將120只SD大鼠隨機(jī)分為四組:空白對照組、TBI組、刺激組(TBI+VNS)和拮抗劑組(TBI+VNS+SB334867)。通過VNS刺激腦外傷后昏迷大鼠,通過意識狀態(tài)六級評分法評估大鼠的行為學(xué)變化,并于刺激結(jié)束后6h、12h、24h,用ELISA、Western-blot、免疫組織化學(xué)技術(shù)檢測各組大鼠前額葉皮質(zhì)Orexin-A和OX1R的表達(dá)。結(jié)果:TBI組中8只(8/30)大鼠出現(xiàn)翻正反射,刺激組中20只(20/30)大鼠出現(xiàn)翻正反射,拮抗劑組中12只(12/30)大鼠出現(xiàn)翻正反射;Orexin-A的含量在各時間點(diǎn)中呈現(xiàn):拮抗劑組空白對照組TBI組刺激組(P0.05),而OX1R的表達(dá)在各時間點(diǎn)缺乏一致性,6h:空白對照組拮抗劑組TBI組刺激組(P0.05);12h:空白對照組TBI拮抗劑組刺激組(P0.05);24h:拮抗劑組空白對照組TBI組刺激組(P0.05)。結(jié)論:VNS可改善腦外傷昏迷大鼠意識狀態(tài)水平,其作用機(jī)制可能與上調(diào)前額葉皮層中Orexin-A和OX1R表達(dá)水平有關(guān)。
[Abstract]:Objective: to investigate the effects of vagus nerve stimulation (VNSs) on the wake-up of rats with traumatic brain injury-induced brain injury (TBI) and the regulatory mechanism of Orexins in the above effects. Methods: 120 Sprague-Dawley rats were randomly divided into four groups: the blank control group, the control group, and the control group. The rats in the stimulation group and the antagonist group were treated with TBI VNS SB334867. The behavioral changes of the rats were evaluated by the six grade score of consciousness state after VNS stimulation. The expressions of Orexin-A and OX1R in the prefrontal cortex of rats in each group were detected with ELISAA Western-blot and immunohistochemistry. Results the positive inversion reflex was found in 8 / 30 of the rats and 20 / 30 / 30 of the rats in the stimulation group. In the antagonist group, the content of Orexin-A appeared in 12 / 30 rats at different time points: in the antagonist group, the level of Orexin-A was found in the blank control group, the stimulation group in the TBI group was P0.05A, and the expression of OX1R was lack of consistency at each time point for 6 h: TBI in the blank control group of antagonist group. The control group was treated with TBI antagonist group for 24 hours. The control group with TBI group was stimulated with P0.05. Conclusion the level of conscious state of coma rats with brain injury can be improved by using the control group (P0.05) for 12 h, and the control group with TBI antagonist group for 24 h, and the control group, TBI group, can improve the level of consciousness state of the rats with brain injury. The mechanism may be related to the up-regulation of Orexin-A and OX1R expression in prefrontal cortex.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 董曉陽;馮珍;;迷走神經(jīng)電刺激促醒作用的研究進(jìn)展[J];中國康復(fù)醫(yī)學(xué)雜志;2017年04期

2 董曉陽;馮珍;;深部腦刺激昏迷促醒靶點(diǎn)的研究進(jìn)展[J];中國康復(fù)理論與實踐;2016年07期

3 董曉陽;馮珍;;迷走神經(jīng)電刺激對腦外傷昏迷大鼠前額葉皮質(zhì)5-羥色胺2A受體表達(dá)的影響[J];中國康復(fù)理論與實踐;2016年04期

4 鐘穎君;馮珍;;正中神經(jīng)電刺激對促醒相關(guān)神經(jīng)遞質(zhì)影響的研究進(jìn)展[J];中國康復(fù)醫(yī)學(xué)雜志;2015年03期

5 魏天祺;馮珍;;迷走神經(jīng)電刺激臨床應(yīng)用及機(jī)制研究進(jìn)展[J];中國康復(fù)醫(yī)學(xué)雜志;2015年02期

6 王鵬;邢紅偉;周志武;;腦外傷致持續(xù)性植物狀態(tài)的催醒治療研究進(jìn)展[J];疑難病雜志;2014年01期

7 趙玉雪;何偉;榮培晶;朱兵;;膽堿能抗炎通路的研究進(jìn)展[J];中華中醫(yī)藥雜志;2013年11期

8 趙彬元;李玉霞;明海霞;陳彥文;梁永林;李楊;吳玉宏;;電刺激單、雙側(cè)迷走神經(jīng)對肝郁證大鼠模型腦內(nèi)NE、DA、5-HT和5-HIAA的影響[J];中醫(yī)研究;2013年01期

9 王亮;馮珍;;神經(jīng)電刺激的臨床應(yīng)用及機(jī)制研究進(jìn)展[J];中國康復(fù)醫(yī)學(xué)雜志;2013年08期

10 喻東山;;睡眠的神經(jīng)遞質(zhì)與精神藥理[J];中國全科醫(yī)學(xué);2010年08期

，

本文編號：1632296