發(fā)布時間：2018-03-18 23:40

  本文選題：低氧血癥　切入點(diǎn)：創(chuàng)傷性軸索損傷　出處：《上海交通大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：研究目的 1.在創(chuàng)傷性軸索損傷(TAI)合并低氧血癥性二次腦損傷（SBI）大鼠模型基礎(chǔ)上進(jìn)行不同氧濃度復(fù)蘇，分析比較不同氧濃度復(fù)蘇之間大鼠動脈血?dú)庵笜?biāo)的變化規(guī)律，探討不同氧濃度對動脈血?dú)獾挠绊憽?2.在創(chuàng)傷性軸索損傷(TAI)合并低氧血癥性二次腦損傷（SBI）大鼠模型基礎(chǔ)上進(jìn)行不同氧濃度復(fù)蘇，分析比較不同氧濃度復(fù)蘇之間對大鼠組織病理學(xué)的變化規(guī)律，探討不同氧濃度對損傷軸索的影響 研究方法 1.采用自制聯(lián)合損傷裝置制作TAI模型，經(jīng)小動物呼吸機(jī)吸入低濃度氧構(gòu)建低氧血癥，從而形成TAI合并低氧血癥的大鼠模型。 2.在此模型基礎(chǔ)上，對氧復(fù)蘇后大鼠動脈血行血?dú)夥治�，研究不同氧濃度�?fù)蘇對大鼠動脈血?dú)庾兓挠绊憽?3.在此模型基礎(chǔ)上，對氧復(fù)蘇后大鼠腦組織行β-APP免疫組化染色,研究不同氧濃度復(fù)蘇對大鼠腦組織病理變化的影響。 研究結(jié)果 1.給予21%氧濃度混合氣體時，PaO2迅速上升，接近正常水平；50%氧濃度時，PaO2上升至正常時的1.8倍；75%氧濃度時，，是正常時的2.0倍；100%氧濃度時是正常時的2.9倍。給予21%氧濃度混合氣體時，SaO2迅速升高，接近正常水平；50%氧濃度時，SaO2接近100%；75%、100%氧濃度時，SaO2為100%。不同氧濃度通氣復(fù)蘇后，即各組內(nèi)致傷后60min、90min（即通氣復(fù)蘇30min、60min）時兩者之間PaO2和SaO2基本一致。 2.延髓部位ROI的β-APP免疫組化染色顯示，24h時TAI合并缺氧組的陽性染色明顯強(qiáng)于各復(fù)蘇組，50%氧濃度時的陽性染色最弱；各組陽性染色隨時間而減弱，1w時各組陽性染色明顯弱于24h，此時TAI合并缺氧組的陽性染色仍明顯強(qiáng)于各復(fù)蘇組，50%氧濃度時的陽性染色最弱。染色陽性的神經(jīng)軸索半定量分析顯示，24h時TAI合并缺氧組明顯高于各復(fù)蘇組，50%氧濃度時的值最低；各組數(shù)值隨時間而降低，1w時各組值明顯低于24h，此時TAI合并缺氧組的值仍高于各復(fù)蘇組，50%氧濃度的值仍為最低。 研究結(jié)論 1、給氧治療可以明顯改善創(chuàng)傷后缺氧大鼠的動脈氧分壓和氧飽和度，而且隨著氧濃度的升高其動脈氧分壓逐漸升高。 2、給氧治療可以顯著減低軸索損傷的程度，起到神經(jīng)保護(hù)作用。 3、對于此TAI合并缺氧動物模型的最佳復(fù)蘇氧濃度為50%。
[Abstract]:Research purpose. 1. On the basis of the model of traumatic axonal injury (TAI) combined with hypoxemia secondary brain injury (SBI), resuscitation with different oxygen concentrations was carried out, and the changes of arterial blood gas indexes in rats with different oxygen concentration resuscitation were analyzed and compared. To study the effect of different oxygen concentration on arterial blood gas. 2. Different oxygen concentration resuscitation was carried out on the basis of traumatic axonal injury (TAI) combined with hypoxemia secondary brain injury (SBI) rat model, and the changes of histopathology between different oxygen concentration resuscitation were analyzed and compared. Study on the effect of different oxygen concentration on damaged axons. Research method. 1. TAI model was made by using self-made combined injury device. Hypoxemia was established by inhaling low concentration oxygen through small animal ventilator, and then the rat model of TAI combined with hypoxemia was established. 2. On the basis of this model, the arterial blood gas of rats after oxygen resuscitation was analyzed to study the effect of different oxygen concentration resuscitation on the changes of arterial blood gas in rats. 3. On the basis of this model, 尾 -APP immunohistochemical staining was used to study the effect of different oxygen concentration resuscitation on the pathological changes of rat brain tissue after oxygen resuscitation. Research results. 1. PaO2 increased rapidly when 21% oxygen concentration was given to mixed gas, and Pao _ 2 increased to 1.8-fold 75% oxygen concentration when it was close to the normal level of 50% oxygen concentration. It is 2. 0 times 100% oxygen concentration at normal level and 2. 9 times as high as 2. 9 times at normal oxygen concentration. When 21% oxygen concentration mixture gas is given, SaO2 increases rapidly, and at 50% oxygen concentration near normal level, SaO2 is close to 100. When oxygen concentration is close to 100%, SaO2 is 100. After different oxygen concentration is resuscitated, In other words, PaO2 and SaO2 were basically the same between the two groups at 60 minutes after injury (30 minutes after resuscitation and 60 minutes after ventilation resuscitation). 2. 尾 -APP immunohistochemical staining of ROI in medulla oblongata showed that the positive staining of TAI combined with hypoxia at 24 h was significantly stronger than that of 50% oxygen concentration in resuscitation group. The positive staining of each group was significantly weaker than that of 24 hours after weakening with time. At this time, the positive staining of TAI combined with hypoxia was still significantly stronger than that of the resuscitation group with 50% oxygen concentration. The neuronal axonal semilayer with positive staining was still stronger than that of the resuscitation group with 50% oxygen concentration. Quantitative analysis showed that TAI combined with hypoxia at 24 h was significantly higher than the lowest at 50% oxygen concentration in each resuscitation group. The values of each group were significantly lower than that of the control group for 24 hours, and the value of TAI combined with hypoxia was still higher than that of the resuscitation group (50% oxygen concentration). Research conclusion. 1. Oxygen administration could significantly improve the arterial oxygen partial pressure and oxygen saturation in rats with post-traumatic hypoxia, and the arterial oxygen partial pressure increased gradually with the increase of oxygen concentration. 2. Oxygen therapy can significantly reduce the degree of axonal injury and play a neuroprotective role. 3. The optimal resuscitation oxygen concentration for this TAI combined with hypoxia animal model is 50.
【學(xué)位授予單位】：上海交通大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R651.15

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱佳駿;吳明遠(yuǎn);;新生兒窒息復(fù)蘇首選純氧還是空氣的Meta分析[J];中華兒科雜志;2007年09期

本文編號：1631885