本文選題：胰島素　切入點(diǎn)：NGF　出處：《中國(guó)修復(fù)重建外科雜志》2013年02期 　論文類(lèi)型：期刊論文

【摘要】：目的制備N(xiāo)GF-胰島素復(fù)合凝膠并觀察其對(duì)大鼠深Ⅱ度燙傷創(chuàng)面愈合的影響。方法以卡波姆980為基質(zhì),分別加入NGF 4 000 U、胰島素800 U,制備胰島素凝膠、NGF凝膠、NGF-胰島素復(fù)合凝膠。觀察NGF-胰島素復(fù)合凝膠性狀,并行體外藥物釋放檢測(cè)。75只SPF級(jí)雄性Wistar大鼠,體重200～250 g,隨機(jī)分為正常對(duì)照組(A組)、糖尿病對(duì)照組(B組)、局部胰島素凝膠治療組(C組)、局部NGF凝膠治療組(D組)、局部NGF-胰島素復(fù)合凝膠治療組(E組),每組15只。B、C、D、E組大鼠采用一次性腹腔注射鏈脲佐菌素(55 mg/kg)制備1型糖尿病模型,A組注射相同劑量檸檬酸鈉緩沖液。造模成功后,采用恒溫水浴箱法于各組大鼠背部制備深Ⅱ度燙傷模型。A、B組創(chuàng)面外敷空白凝膠基質(zhì),C、D、E組對(duì)應(yīng)外敷胰島素凝膠、NGF凝膠、NGF-胰島素復(fù)合凝膠,每天換藥1次。燙傷后觀察各組大鼠存活情況,于3、7、11、15、21 d觀察創(chuàng)面愈合情況并計(jì)算創(chuàng)面愈合率,各時(shí)間點(diǎn)每組處死3只大鼠取皮膚全層標(biāo)本行組織學(xué)及免疫組織化學(xué)染色觀察。結(jié)果制備的NGF-胰島素復(fù)合凝膠清亮透明,保濕性及黏附性良好,易于涂抹及清洗。體外釋放檢測(cè)示NGF-胰島素復(fù)合凝膠釋藥時(shí)間可達(dá)24 h以上,且30 d內(nèi)穩(wěn)定性良好。各組大鼠均存活至實(shí)驗(yàn)完成。燙傷后3 d各組創(chuàng)面無(wú)縮小,7、11、15、21 d時(shí)E組創(chuàng)面愈合率最高,B組最低,與其余各組比較以及E、B組間比較,差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。組織學(xué)觀察示各時(shí)間點(diǎn)E組肉芽組織和膠原纖維生長(zhǎng)均優(yōu)于其余各組。免疫組織化學(xué)染色示各組CD34、增殖細(xì)胞核抗原(proliferating cell nuclear antigen,PCNA)均于3 d開(kāi)始表達(dá),且隨時(shí)間延長(zhǎng)陽(yáng)性細(xì)胞逐漸增多;各時(shí)間點(diǎn)E組微血管密度及PCNA最高,B組最低,與其余各組比較以及E、B組間比較差異均有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論局部應(yīng)用NGF-胰島素復(fù)合凝膠可顯著促進(jìn)大鼠深Ⅱ度燙傷創(chuàng)面愈合。
[Abstract]:Objective to prepare NGF- insulin composite gel and observe its effect on wound healing of deep second degree scald in rats. Methods Carbomer 980 was used as the matrix. NGF 4 000 U and insulin 800 U were added to prepare the NGF- insulin composite gel. The characters of NGF- insulin compound gel were observed, and the drug release in vitro was performed in 75 male Wistar rats of SPF grade. Weighing 200g, they were randomly divided into normal control group (n = 15), diabetes control group (n = 15), local insulin gel group (n = 15), local insulin gel group (n = 15), group C (n = 15), local NGF gel group (n = 15) and group E (n = 15). Rats in group A were injected with sodium citrate buffer at the same dose by intraperitoneal injection of streptozotocin (55 mg / kg). The deep 鈪，

本文編號(hào)：1618003