本文關鍵詞： 微小RNA- 烏司他丁 炎性反應　出處：《重慶醫(yī)科大學學報》2017年05期 　論文類型：期刊論文

【摘要】：目的:觀察在脂多糖(lipopolysaccharide,LPS)誘導的小鼠巨噬細胞株RAW264.7產(chǎn)生炎癥反應中微小RNA-21(Micro RNA-21,mi R-21)的表達情況,以及烏司他丁(Ulinastatin,UTI)在炎癥反應中對mi R-21的干預作用。方法:設置正常組、模型組(LPS 500 ng/m L剌激組)、實驗組(LPS 500 ng/m L+UTI 1 000 U/m L組)和陰性實驗對照組(UTI 1 000 U/m L),觀察RAW264.7細胞生長狀態(tài);四甲基偶氮唑(methyl thiazolyl tetrazolium,MTT)法檢測不同濃度UTI對RAW264.7細胞活性的影響;用不同濃度UTI預處理2 h后,LPS刺激小鼠RAW264.7細胞誘導炎癥模型,RT-PCR檢測腫瘤壞死因子-α(tumor necrosis factor-α,TNF-α)m RNA、白介素-6(interleukin-6,IL-6)m RNA、mi R-21的表達。結果:(1)UTI對RAW264.7細胞的毒性作用:UTI濃度小于1 000 U/m L時對RAW264.7細胞無毒性作用,比較差異無統(tǒng)計學意義(P=0.117)。(2)與正常組相比,在不同濃度(100,250,500 ng/m L)LPS刺激RAW264.7細胞后,TNF-αm RNA及IL-6 m RNA的分泌(1.311±0.037,1.549±0.039,1.667±0.059;1.167±0.019,1.518±0.058,1.740±0.071)與基礎分泌量(1.000±0.000)相比明顯升高(P=0.000),且mi R-21表達(1.082±0.114,1.671±0.087,2.789±0.107)明顯升高,并隨LPS濃度升高而增加(P=0.000);(3)不同濃度UTI(10,100,1 000 U/m L)能有效降低TNF-αm RNA及IL-6 m RNA(1.000±0.000,1.998±0.212,1.421±0.100,1.122±0.154,0.991±0.139;1.000±0.000,1.880±0.045,1.179±0.108,1.053±0.070,0.960±0.088)的表達,并降低mi R-21(1.000±0.000,2.789±0.107,2.675±0.135,2.258±0.170,1.369±0.279)的表達,差異有統(tǒng)計學意義(P1=0.000,P2=0.000,P3=0.000)。結論:LPS刺激小鼠RAW264.7細胞可促進mi R-21表達升高,其表達水平在一定程度上反映了細胞炎癥反應狀態(tài);UTI可通過下調(diào)mi R-21抑制相關信號通路從而在免疫炎癥反應中發(fā)揮保護作用。
[Abstract]:Aim: to observe the expression of minimal RNA-21(Micro RNA-21 in lipopolysaccharide lipopolysaccharide (LPS) -induced inflammatory reaction in murine macrophage cell line RAW264.7, and the effect of ulinastatin Ulinastatinatin UTII on the inflammatory response of murine macrophage cell line RAW264.7. Methods: normal control group was set up. The growth status of RAW264.7 cells was observed in the model group stimulated with LP500 ng/m L, the experimental group in the LP500 ng/m L UTI 1 000 U / mL group and the negative control group in the control group. The effects of different concentrations of UTI on the cell activity of RAW264.7 were detected by the method of tetrazoliumium tetrazolium tetrazolium tetrazolium (RAW264.7). After pretreatment with different concentrations of UTI for 2 h, the inflammatory model induced by RAW264.7 cells was pretreated with different concentrations of UTI. RT-PCR was used to detect the expression of tumor necrosis factor- 偽 -tumor necrosis factor- 偽 tNF- 偽 mRNAs and interleukin-6 interleukin-6m RNAi R-21. Results the toxic effect of UTI on RAW264.7 cells was less than 1 000 UMT L ~ (-1) 路min ~ (-1) ~ (-1) 路min ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1) ~ (-1). It has no toxic effect on RAW264.7 cells. There was no significant difference between the two groups.) compared with the control group, the secretion of TNF- 偽 m RNA and IL-6 m RNA in RAW264.7 cells stimulated by LPs at different concentrations was significantly higher than that in the control group (1.311 鹵0.0377.49 鹵0.0399.1.667 鹵0.0591.167 鹵0.0191.518 鹵0.0581.740 鹵0.0771), and the expression of R-21 was 1.082 鹵0.1141.671 鹵0.082.789 鹵0.107), and the expression of R-21 was 1.082 鹵0.1141.671 鹵0.072.789 鹵0.107). With the increase of LPS concentration, the expression of TNF- 偽 m RNA and IL-6 m RNA(1.000 鹵0.000UmL) was significantly decreased. The difference was statistically significant (P < 0.01 0. 091 鹵0. 1391.000 鹵0. 1391.000 鹵0. 880 鹵0. 1081.179 鹵0. 1081.053 鹵0. 0700.60 鹵0. 088)), and decreased the expression of TNF- 偽 m RNA and IL-6 m RNA(1.000 鹵0. 10770 鹵2. 675 鹵0. 1352.258 鹵0. 1700.369 鹵0. 279). Conclusion LPS can stimulate the expression of TNF- 偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can stimulate the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can promote the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 in mice. Conclusion LPS can promote the expression of TNF- 偽-偽 RNA and IL-6 m RNA(1.000 + 0.1070.675 鹵0.1352.258 鹵0.1752.258 鹵0.1700.369 鹵0.2799.Conclusion LPS can promote the expression of TNF- 偽 RNA and IL-6 m RNA(1.000 in mice. To a certain extent, the expression level of UUTI may play a protective role in the immune inflammatory response by down-regulating the signal pathway associated with the inhibition of miR-21.
【作者單位】： 重慶醫(yī)科大學附屬第一醫(yī)院重癥醫(yī)學科;重慶市急救醫(yī)療中心ICU;
【基金】：重慶市衛(wèi)生計生委科研資助項目(編號:2016ZDXM030)
【分類號】：R459.7

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