發(fā)布時間：2019-05-12 19:11

【摘要】：目的:建立小鼠肝癌休眠模型并驗證休眠細胞的存在。方法:30只昆明種小鼠均右側腋下接種1×106個H22腹水型肝癌細胞,常規(guī)喂養(yǎng)2周,第15天選擇表皮腫瘤直徑0.5cm的小鼠,進行姑息性手術,將肉眼可見腫瘤組織全部切除。手術后的小鼠再常規(guī)喂養(yǎng)8周,如接種處未見腫瘤組織生長,即認為肝癌小鼠休眠模型建立成功(按照平均壽命折算,小鼠生存期8周,即相當于人類壽命5年)。其后模型小鼠分為復發(fā)對照組和復發(fā)實驗組,復發(fā)對照組小鼠常規(guī)喂養(yǎng);復發(fā)實驗組模型小鼠給予連續(xù)外傷刺激。6周后處死全部模型小鼠,接種部位取材,組織固定和HE染色觀察腫瘤再生成情況確定休眠細胞的存在。結果:肝癌細胞接種于小鼠并行手術后,有20%小鼠成瘤,80%處于休眠狀態(tài)。復發(fā)實驗組給予連續(xù)外傷刺激6周后,100%小鼠成瘤,而復發(fā)對照組只有8.3%,兩組差異有統(tǒng)計學意義,P=0.000。結論:本方法成功建立了小鼠肝癌休眠模型,并用外傷刺激引起肝癌休眠細胞增殖,驗證了休眠腫瘤細胞在體內的存在。
[Abstract]:Objective: to establish a dormant model of liver cancer in mice and verify the existence of dormant cells. Methods: 30 Kunming mice were inoculated with 1 脳 106 H22 aspartate liver cancer cells under the right armpit and fed for 2 weeks. On the 15th day, the mice with 0.5cm in diameter of epidermis were selected for palliative operation and all the tumor tissues were resected with naked eye. If no tumor tissue growth was found in the inoculated mice, the dormancy model of liver cancer mice was established successfully (according to the average life expectancy, the survival time of mice was 8 weeks, that is, equivalent to 5 years of human life span). The model mice were divided into recurrent control group and recurrent experimental group, and the recurrent control group mice were fed with routine feeding. The model mice in the recurrent experimental group were stimulated by continuous trauma. After 6 weeks, all the model mice were killed and taken from the inoculated site. Tissue fixation and HE staining were used to observe the regeneration of the tumor to determine the existence of dormant cells. Results: after the liver cancer cells were inoculated in mice and operated, 20% of the mice formed tumors and 80% of them were dormant. After 6 weeks of continuous trauma stimulation, 100% of the mice in the recurrent experimental group formed tumors, while only 8.3% in the recurrent control group. There was significant difference between the two groups (P 鈮，

本文編號：2475629