【摘要】：為進(jìn)行傳染性法氏囊病病毒(IBDV)多表位抗原的原核表達(dá)和免疫特性分析,應(yīng)用重疊延伸PCR技術(shù)擴(kuò)增合成由病毒VP2和VP3蛋白多個(gè)表位序列構(gòu)成的重組抗原基因,并將抗原基因與原核表達(dá)載體pBVIL1重組,表達(dá)多表位融合抗原,通過與標(biāo)準(zhǔn)抗血清反應(yīng)和動(dòng)物免疫分別檢測表達(dá)抗原的免疫反應(yīng)性和免疫原性。結(jié)果顯示,經(jīng)42℃誘導(dǎo)后,含重組載體的大腸桿菌表達(dá)了31 kD的抗原蛋白,Western blot鑒定出現(xiàn)陽性條帶,免疫小鼠后,抗血清的滴度為1∶6 400。說明原核表達(dá)載體構(gòu)建成功,大量表達(dá)的多表位融合抗原具有IBDV抗原反應(yīng)性。
[Abstract]:In order to analyze the prokaryotic expression and immunological characteristics of (IBDV) polyepitope antigen of infectious bursal disease virus (IBDV), a recombinant antigen gene composed of multiple epitopes of VP2 and VP3 proteins was synthesized by overlapping extended PCR technique. The antigen gene was recombined with prokaryotic expression vector pBVIL1 to express multi-epitope fusion antigen. The immunoreactivity and immunogenicity of the expressed antigen were detected by reaction with standard antiserum and animal immunity. The results showed that after induction at 42 鈩，

本文編號：2424717