【摘要】：為進行傳染性法氏囊病病毒(IBDV)多表位抗原的原核表達和免疫特性分析,應用重疊延伸PCR技術擴增合成由病毒VP2和VP3蛋白多個表位序列構成的重組抗原基因,并將抗原基因與原核表達載體pBVIL1重組,表達多表位融合抗原,通過與標準抗血清反應和動物免疫分別檢測表達抗原的免疫反應性和免疫原性。結果顯示,經(jīng)42℃誘導后,含重組載體的大腸桿菌表達了31 kD的抗原蛋白,Western blot鑒定出現(xiàn)陽性條帶,免疫小鼠后,抗血清的滴度為1∶6 400。說明原核表達載體構建成功,大量表達的多表位融合抗原具有IBDV抗原反應性。
[Abstract]:In order to analyze the prokaryotic expression and immunological characteristics of (IBDV) polyepitope antigen of infectious bursal disease virus (IBDV), a recombinant antigen gene composed of multiple epitopes of VP2 and VP3 proteins was synthesized by overlapping extended PCR technique. The antigen gene was recombined with prokaryotic expression vector pBVIL1 to express multi-epitope fusion antigen. The immunoreactivity and immunogenicity of the expressed antigen were detected by reaction with standard antiserum and animal immunity. The results showed that after induction at 42 鈩，

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