【摘要】：目的探討小鼠胚胎心神經(jīng)嵴細(xì)胞的形成、分布模式及其在心血管系統(tǒng)發(fā)育過程中的作用。方法選用抗細(xì)胞視黃酸結(jié)合蛋白1(CRABP1)、抗α-平滑肌肌動(dòng)蛋白(α-SMA)、抗心肌肌球蛋白重鏈(MHC)、抗胰島因子1(Isl-1)抗體,對(duì)45只胚齡8～12d小鼠胚胎連續(xù)切片進(jìn)行免疫組織化學(xué)染色。結(jié)果胚齡8d,CRABP1在神經(jīng)褶的外胚層未見陽性表達(dá)。胚齡8.5～9d,在心管與鰓弓水平,神經(jīng)褶開始出現(xiàn)CRABP1陽性細(xì)胞,且有部分細(xì)胞從神經(jīng)褶背側(cè)分離進(jìn)入鄰近間充質(zhì)。胚齡10d,神經(jīng)管兩側(cè)間充質(zhì)內(nèi)的CRABP1陽性細(xì)胞遷移至鰓弓、弓動(dòng)脈壁內(nèi)皮周圍以及流出道心膠質(zhì)內(nèi)。胚齡11～12d,弓動(dòng)脈內(nèi)皮周圍、流出道心內(nèi)膜墊內(nèi)CRABP1表達(dá)明顯下降,但弓動(dòng)脈管壁α-SMA陽性平滑肌細(xì)胞數(shù)量增加。主肺動(dòng)脈隔及其分隔形成的升主動(dòng)脈和肺動(dòng)脈干管壁內(nèi)均可見Isl-1陽性細(xì)胞,但未見CRABP1表達(dá)。結(jié)論小鼠胚胎CRABP1陽性神經(jīng)嵴細(xì)胞形成的時(shí)間窗限定在胚齡8.5～9d。胚齡10d后,CRABP1陽性神經(jīng)嵴細(xì)胞經(jīng)過遷移,參與弓動(dòng)脈中膜平滑肌和流出道心內(nèi)膜墊的形成。CRABP1不能用于標(biāo)記遷移后的神經(jīng)嵴細(xì)胞。
[Abstract]:Objective to investigate the formation and distribution pattern of cardiac crest cells in mouse embryo and its role in the development of cardiovascular system. Methods Anti cell retinoic acid binding protein-1 (CRABP1) and 偽 -smooth muscle actin (偽-SMA),) anti-cardiac myosin heavy chain (MHC), anti-islet factor 1 (Isl-1) antibody were used. The embryo sections of 45 812 day old mice were stained with immunohistochemistry. Results there was no positive expression of CRABP1 in the neuropleated ectoderm at 8 d of embryo. At the age of 8.5 ~ 9 days, at the level of cardiac tube and Gill arch, CRABP1 positive cells began to appear in the pleats, and some cells were isolated from the dorsal side of the pleats into the adjacent mesenchymal cells. At the age of 10 days, the CRABP1 positive cells in the mesenchymal cells on both sides of the neural tube migrated to the Gill arch, the wall of the arcuate artery around the endothelium and the cardiac glia of the outflow tract. At the age of 11 ~ 12 days, the expression of CRABP1 in the endocardium of the outflow tract decreased significantly around the endothelium of the arch artery, but the number of 偽-SMA positive smooth muscle cells increased in the wall of the arch artery. Isl-1 positive cells were found in the walls of ascending aorta and pulmonary trunk, but no expression of CRABP1 was found in the septum of aorta and its septum. Conclusion the time window for the formation of CRABP1 positive neural crest cells in mouse embryos is limited to 8. 5 days. After 10 days of embryo age, CRABP1 positive neural crest cells migrated and participated in the formation of medial smooth muscle and endocardial cushion of outflow tract. CRABP1 could not be used to label the migrated neural crest cells.
【作者單位】： 山西醫(yī)科大學(xué)組織學(xué)胚胎學(xué)教研室;渭南職業(yè)技術(shù)學(xué)院護(hù)理系;
【基金】：國家自然科學(xué)基金資助項(xiàng)目(30771141) 山西省自然科學(xué)基金資助項(xiàng)目(2006011108) 山西省回國留學(xué)人員科研資助項(xiàng)目(2008-47)
【分類號(hào)】：R329.2

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