本文選題：內(nèi)皮源性降鈣素基因相關(guān)肽 + 人臍靜脈內(nèi)皮細(xì)胞�。� 參考：《中國(guó)現(xiàn)代醫(yī)學(xué)雜志》2013年24期

【摘要】：目的探討內(nèi)皮源性降鈣素基因相關(guān)肽對(duì)血管緊張素II誘導(dǎo)的內(nèi)皮細(xì)胞抗凋亡作用。方法培養(yǎng)HUVEC-12,分別加入不同劑量的AngII孵育內(nèi)皮細(xì)胞,研究其量效和時(shí)效關(guān)系,并給予特異性AT1受體阻斷劑氯沙坦研究其可能的機(jī)制。Real time-PCR檢測(cè)內(nèi)皮細(xì)胞中CGRP mRNA水平;放射免疫法檢測(cè)內(nèi)皮細(xì)胞CGRP的含量。在培養(yǎng)HUVEC-12給予AngII誘導(dǎo)細(xì)胞凋亡,外源性給予CGRP和辣椒素。Annexin V-FITC流式細(xì)胞檢測(cè)細(xì)胞凋亡;比色法測(cè)定caspase-3活性;RT-PCR檢測(cè)內(nèi)皮細(xì)胞中CGRP、Bcl-2和Bax mRNA的表達(dá)。結(jié)果①AngII孵育內(nèi)皮細(xì)胞可劑量和時(shí)間依賴性地降低α-和β-CGRP mRNA的表達(dá)水平和CGRP含量,此作用可被預(yù)先給予的氯沙坦阻斷。②CGRP可劑量依賴性降低AngII誘導(dǎo)的內(nèi)皮細(xì)胞凋亡,減弱AngII增加內(nèi)皮細(xì)胞caspase-3活性,此作用能被預(yù)先給予的CGRP受體阻斷劑CGRP8-37取消。③辣椒素可劑量依賴性上調(diào)內(nèi)皮源性α-和β-CGRP mRNA的表達(dá)水平,此作用能被預(yù)先給予的VR1受體阻斷劑capsazepine取消。④辣椒素可劑量依賴性降低AngII誘導(dǎo)的內(nèi)皮細(xì)胞凋亡,減弱AngII增加內(nèi)皮細(xì)胞caspase-3活性,此作用能被預(yù)先給予的capsazepine和CGRP8-37取消。⑤辣椒素可以上調(diào)抗凋亡分子Bcl-2 mRNA的表達(dá),下調(diào)促凋亡分子Bax mRNA的表達(dá),此作用能被預(yù)先給予的capsazepine和CGRP8-37取消。結(jié)論 AngⅡ能抑制內(nèi)皮細(xì)胞合成與釋放CGRP;用辣椒素促進(jìn)內(nèi)皮源性CGRP的表達(dá)能對(duì)抗AngⅡ誘導(dǎo)的內(nèi)皮細(xì)胞凋亡。
[Abstract]:Objective to investigate the antiapoptotic effect of endothelium-derived calcitonin gene-related peptide on angiotensin II induced endothelial cells. Methods HUVEC-12 was incubated with different doses of AngII to investigate the dose-effect and time-dependent relationship, and the possible mechanism of Losartan, a specific AT1 receptor blocker, was studied. Real time-PCR was used to detect the level of CGRP mRNA in endothelial cells. The content of CGRP in endothelial cells was detected by radioimmunoassay. Apoptosis was induced by AngII in cultured HUVEC-12, apoptosis was detected by CGRP and capsaicin. Annexin V-FITC flow cytometry was used to detect apoptosis, and caspase-3 activity was detected by RT-PCR to detect the expression of Bcl-2 and Bax mRNA in endothelial cells. Results the expression of 偽-and 尾 -CGRP mRNA and the content of CGRP were decreased in a dose-and time-dependent manner after 1AngII incubation, which could be blocked by losartan in a dose-dependent manner, and the apoptosis of endothelial cells induced by AngII was decreased in a dose-dependent manner. Attenuated AngII increased caspase-3 activity in endothelial cells, which could be inhibited by CGRP receptor blocker CGRP8-37. 3 capsaicin could up-regulate the expression of endothelium-derived 偽-and 尾 -CGRP mRNA in a dose-dependent manner. This effect could be eliminated by pre-administered VR1 receptor antagonist capsazepine. Capsaicin could decrease the apoptosis of endothelial cells induced by AngII in a dose-dependent manner, and decrease the increase of caspase-3 activity of endothelial cells induced by AngII. This effect can be cancelled by pre-administration of capsazepine and CGRP8-37. 5 capsaicin can up-regulate the expression of anti-apoptotic molecule Bcl-2 mRNA and down-regulate the expression of pro-apoptotic molecule Bax mRNA. This effect can be cancelled by pre-administered capsazepine and CGRP8-37. Conclusion Ang 鈪，

本文編號(hào)：1941000