本文選題：Myroides + odoratimimus　； 參考：《華僑大學(xué)》2017年碩士論文

【摘要】：Myroides sp.菌,原名芳香黃桿菌(Flavobacterium odoratum),是非發(fā)酵性、專性需氧型、革蘭氏陰性不動桿菌,在代謝過程中能產(chǎn)生具有水果香味的黃色物質(zhì)。Myroides sp.能感染免疫力低下的人群,造成各種院內(nèi)感染甚至爆發(fā)感染,如:泌尿系統(tǒng)感染、蜂窩組織炎、菌血癥、惡性腫瘤、壞死性筋膜炎、敗血癥、心室炎和糖尿病并發(fā)癥等。Myroides sp.感染難治愈,因為Myroides sp.菌耐藥性廣泛,且抗生素耐藥機制尚不清楚。本論文對一株臨床分離的多耐藥Myroides odoratimimus(M.odoratimimus)菌PR63039,采用PacBio RSII測序系統(tǒng),對其進(jìn)行全基因組測序和生物信息學(xué)分析,試圖在基因組水平闡明M.odoratimimus PR63039的抗生素耐藥和致病機制、并比較臨床M.odoratimimus分離菌株基因組之間的異同。M.odoratimimus PR63039基因組由染色體(4,366,950 bp)和質(zhì)粒(90,798bp)組成。使用CG viewer,我們獲得PR63039的基因組圈圖。PR63039基因組含有大量的耐藥基因,包括:β-內(nèi)酰胺耐藥基因、四環(huán)素耐藥基因、氯霉素耐藥基因、喹諾酮耐藥基因、多耐藥質(zhì)子泵基因、耐藥突變基因Staphylococcus aureus rpoB和Pseudomonas aeruginosa gyrA。該菌耐藥基因的分布是獨特的,我們發(fā)現(xiàn)了一個耐藥區(qū)域,并將其命名為MY63039-RR。通過分析可知,菌株P(guān)R63039抗生素耐藥基因與藥敏試驗(AST)中的耐藥譜表型基本一致。對M.odoratimimus PR63039的基因組分析部分澄清了其抗生素多耐藥機制。此外,M.odoratimimus PR63039基因組中還預(yù)測到38個毒力因子和兩個原噬菌體。四株臨床M.odoratimimus菌株(M.odoratimimus PR63039、M.odoratimimus CCUG10230、M.odoratimimus CCUG12901、M.odoratimimus CIP101113)之間的比較基因組學(xué)分析結(jié)果表明,它們的基因組同源性比較大。系統(tǒng)發(fā)育樹顯示菌株CCUG10230、CCUG12901和CIP101113可能屬于相同的克隆。所有菌株基因組中均存在多種抗生素耐藥基因,CCUG12901、CIP101113和CCUG10230中預(yù)測到的耐藥基因幾乎一致。共線性分析表明四個基因組是非常相似的,即使存在一些染色體重排。通過CRISPR預(yù)測分析,我們發(fā)現(xiàn)菌株P(guān)R63039含有三種類型的CRISPR,CCUG10230含有四種類型的CRISPR,但菌株CCUG12901和CIP101113的基因組中無CRISPR序列。此外,所有四株臨床分離菌基因組中均預(yù)測到原噬菌體序列,盡管原噬菌體組成元件略有差別�？傮w上,該論文在基因組水平上部分闡明了M.odoratimimus PR63039抗生素多耐藥機制、致病力機制、4株臨床M.odoratimimus致病菌之間的同源性。這些基因組數(shù)據(jù)和發(fā)現(xiàn)為臨床M.odoratimimus菌感染的治療和新抗生素研發(fā)提供了理論指導(dǎo)。
[Abstract]:Myroides sp. Flavobacterium odoratumum, which is non-fermentative, specific aerobic, Gram-negative Acinetobacter sp. can produce the yellow substance. Myroides sp. in the metabolic process. It can infect people with low immunity, causing various nosocomial infections and even erupting infections, such as urinary tract infection, cellulitis, bacteremia, malignant tumor, necrotizing fasciitis, septicemia, ventricular inflammation and diabetic complications, etc. Myroides spp. Infection is difficult to cure because of Myroides sp. Antibiotic resistance is widespread and the mechanism of antibiotic resistance is unclear. In this paper, a clinical isolates of multidrug resistant Myroides odoratimim M. odoratimimia PR63039 were sequenced by PacBio RSII sequencing system. The whole genome of PR63039strain was sequenced and bioinformatics analysis was carried out to elucidate the mechanism of antibiotic resistance and pathogenesis of M.odoratimimus PR63039 at the genomic level. The genome of M. odoratimimus was composed of chromosome 4366950bpand plasmid 90798bp. Using CG viewer, we obtained that the genome of PR63039, PR63039, contained a large number of drug-resistant genes, including 尾 -lactam resistance, tetracycline resistance, chloramphenicol resistance, quinolone resistance, and multidrug resistance proton pump genes. Staphylococcus aureus rpoB and Pseudomonas aeruginosa gyrA. The distribution of drug resistance gene was unique. We found a drug resistance region and named it MY63039-RR. The results showed that the antibiotic resistance genes of the strain PR63039 were basically consistent with the drug resistance patterns in the drug sensitivity test (AST). The genome analysis of M.odoratimimus PR63039 clarifies the mechanism of multidrug resistance of M.odoratimimus. In addition, 38 virulence factors and two prophages were predicted in the genome of M. odoratimimus PR63039. The comparative genomics analysis between four clinical M.odoratimimus strains M. odoratimimus PR63039 and M. odoratimimus CCUG10230 (M.odoratimimus CCUG12901, M.odoratimimus CIP101113) showed that their genomes were homologous. Phylogenetic tree showed that CCUG10230 CCUG12901 and CIP101113 might belong to the same clone. All strains had multiple antibiotic resistance genes CCUG12901CIP101113 and the predicted resistance genes in CCUG10230 were almost the same. Co-linear analysis showed that the four genomes were very similar, even if there were some chromosome rearrangements. By CRISPR prediction analysis, we found that the strain PR63039 contained three types of CRISPRA CCUG10230, but no CRISPR sequence in the genome of CCUG12901 and CIP101113. In addition, prophage sequences were predicted in the genomes of all four clinical isolates, although the composition of the phage was slightly different. In general, at the genomic level, the mechanism of multidrug resistance of M.odoratimimus PR63039 and the homology of four clinical M.odoratimimus pathogens were elucidated in this paper. These genomic data and findings provide theoretical guidance for the treatment of clinical M.odoratimimus infection and the development of new antibiotics.
【學(xué)位授予單位】：華僑大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R378

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