本文選題：特應(yīng)性皮炎　切入點(diǎn)：VDRAs　出處：《天津醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的 觀察在特應(yīng)性皮炎(AD)小鼠模型中維生素D受體激動劑(VDRAs)的干預(yù)對皮損以及Toll受體2、Toll受體4(TLR2、TLR4)和鼠陽離子抗菌肽CRAMP的影響,并探討其相互關(guān)聯(lián)機(jī)制。方法 把25只雌性BALB/c小鼠隨機(jī)劃分為5組,其分別為空白組、AD模型組、AD模型不干預(yù)組、PBS對照組、VDRAs治療組,每組分別為5只。除空白組以外的其余4組,均用二硝基氯苯(DNCB)誘導(dǎo)小鼠特應(yīng)性皮炎模型。AD造模成功后,模型組小鼠直接處死,AD模型不干預(yù)組造模完成后不予以任何處理,VDRAs治療組小鼠腹腔注射VDRAs液,PBS對照組小鼠腹腔注射PBS液,兩組均為每3日注射1次,總計(jì)5次。實(shí)驗(yàn)干預(yù)完成后,對各組小鼠背部皮損的改變情況進(jìn)行觀察,隨后處死小鼠取背部皮膚組織做組織病理切片和免疫組化,用顯微鏡觀察各組小鼠背部皮損處的組織改變情況,免疫組化染色后觀察各組小鼠TLR2、TLR4及C RAMP在皮損中的表達(dá)水平和表達(dá)定位,Real Time PCR檢測各組小鼠皮損中TLR2、TLR4及CRAMP的RNA含量并定量分析其變化。結(jié)果 VDRAs治療組小鼠皮損較AD模型不干預(yù)組和PBS對照組明顯改善,免疫組化和Real-Time PCR結(jié)果示VDRAs治療組小鼠皮損中CRAMP的表達(dá)高于AD模型不干預(yù)組和PBS對照組,TLR2和TLR4的表達(dá)較PBS對照組和AD模型組低,其差異有統(tǒng)計(jì)學(xué)意義(P0.01)。結(jié)論 在AD小鼠模型中,VDRAs可抑制TLR2、TLR4表達(dá),上調(diào)鼠陽離子抗菌肽CRAMP的表達(dá)。
[Abstract]:Objective To observe the effect on atopic dermatitis (AD) in a mouse model of vitamin D receptor agonist (VDRAs) intervention on lesions and Toll 2 receptor, Toll receptor 4 (TLR2, TLR4) and the effect of rat cationic antimicrobial peptide CRAMP, and to explore the correlation mechanism. Methods 25 female BALB/c mice were randomly divided into the 5 groups were control group, AD model group, AD model group, PBS control group, VDRAs treatment group, each group was 5. In addition to the blank group of the other 4 groups, with two nitrochlorobenzene (DNCB) induced by atopic dermatitis model.AD after successful modeling, model groups of mice were executed, the AD model is not the intervention group after modeling without any treatment, VDRAs treatment group were injected with VDRAs solution, the PBS control group were injected with PBS solution, two groups were injected 1 times every 3 days, a total of 5 times. After the completion of the experimental intervention, the changes of mice back skin the Observe, then the mice were killed to take back the skin tissue for pathology and immunohistochemistry, microscope observed mice back skin lesions tissue changes were observed after TLR2 immunohistochemical stain, expression level in the lesions and the localization of TLR4 and C RAMP, TLR2 Real Time PCR lesions were detected in the analysis of TLR4 and CRAMP, change of RNA content and quantitative. Results VDRAs mice treated with AD model lesions of non intervention group and PBS control group improved significantly, the expression of immunohistochemistry and Real-Time PCR results showed that VDRAs treatment of CRAMP mice in the lesions was higher than that of AD model group and PBS control group, the expression of TLR2 and TLR4 compared with PBS control group and AD model group, the difference was statistically significant (P0.01). Conclusion in a mouse model of AD, VDRAs can inhibit the expression of TLR4, TLR2, expression of rat cationic antimicrobial peptide CRAMP.

【學(xué)位授予單位】：天津醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R758.2;R-332

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