發(fā)布時(shí)間：2018-02-24 18:31

  本文關(guān)鍵詞： EMMPRIN 梗阻性腎病 腎間質(zhì)纖維化 上皮間質(zhì)轉(zhuǎn)分化　出處：《細(xì)胞與分子免疫學(xué)雜志》2013年02期 　論文類型：期刊論文

【摘要】：目的檢測(cè)細(xì)胞外基質(zhì)金屬蛋白酶誘導(dǎo)因子(EMMPRIN)在大鼠腎間質(zhì)纖維化模型中的表達(dá)變化。方法 64只Sprague-Dawley大鼠隨機(jī)分為假手術(shù)組(Sham組)和模型組(UUO組),每組16只。假手術(shù)組,僅游離輸尿管不結(jié)扎,模型組,行單側(cè)輸尿管結(jié)扎術(shù),分別于術(shù)后3、7、14 d處死大鼠并留取腎臟組織。腎臟標(biāo)本行HE、Masson染色方法檢測(cè)間質(zhì)纖維化損傷的程度。應(yīng)用免疫組織化學(xué)方法檢測(cè)腎組織中EMMPRIN,α-SMA,E-cadherin的表達(dá)。結(jié)果 HE染色顯示:UUO術(shù)后腎間質(zhì)腎小管萎縮、擴(kuò)張,胞外基質(zhì)沉積,炎細(xì)胞浸潤(rùn)增加。Masson染色顯示:UUO術(shù)后大鼠腎組織膠原纖維組織增生,出現(xiàn)明顯的間質(zhì)纖維化改變。免疫組織化學(xué)結(jié)果顯示:與假手術(shù)組相比,UUO模型3 d組的EMMPRIN表達(dá)顯著增高,同時(shí)有α-SMA表達(dá)升高和E-cadherin表達(dá)顯著下降(P0.05);隨著梗阻時(shí)間的延長(zhǎng),模型7 d組EMMPRIN的表達(dá)逐漸減弱(P0.05)。結(jié)論EMMPRIN可能參與了UUO大鼠腎間質(zhì)纖維化的早期上皮間質(zhì)轉(zhuǎn)分化過(guò)程,而隨著纖維化程度加重,后期表達(dá)逐漸減少。
[Abstract]:Objective to investigate the expression of extracellular matrix metalloproteinase-inducible factor (EMMPRIN) in rat renal interstitial fibrosis model. Methods Sixty-four Sprague-Dawley rats were randomly divided into sham-operated group (Sham group) and model group (UUO group, 16 rats in each group). The model group was treated with unilateral ureteral ligation. The rats were killed on the 14th day after operation, and the renal tissue was taken. The degree of interstitial fibrosis was detected by HEMA Masson staining. The expression of EMMPRIN, 偽 -SMA-E-cadherin in renal tissue was detected by immunohistochemical method. The results showed that the expression of EMMPRIN, 偽 -SMA-E-cadherin in renal tissue was detected by HE staining. It shows that the renal tubules atrophy in the renal interstitium after the operation of the UUO, Dilatation, deposition of extracellular matrix, increased infiltration of inflammatory cells. Masson staining showed the proliferation of collagen fibers in renal tissue of rats after WUO. The results of immunohistochemistry showed that the expression of EMMPRIN was significantly increased, and the expression of 偽 -SMA and E-cadherin decreased significantly in the 3-day group compared with the sham-operated group, and the expression of E-cadherin decreased significantly with the prolongation of the time of obstruction, and the expression of 偽 -SMA and E-cadherin decreased significantly with the prolongation of the time of obstruction. Conclusion EMMPRIN may be involved in the process of early epithelial stromal transdifferentiation of renal interstitial fibrosis in UUO rats, but the expression decreases gradually with the severity of fibrosis.
【作者單位】： 第四軍醫(yī)大學(xué)西京醫(yī)院腎臟內(nèi)科;
【基金】：西京醫(yī)院學(xué)科助推計(jì)劃(XJZT09D06)
【分類號(hào)】：R363

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