本文關(guān)鍵詞： 樹突狀細(xì)胞 誘導(dǎo) 鑒定　出處：《華中科技大學(xué)學(xué)報(醫(yī)學(xué)版)》2013年04期 　論文類型：期刊論文

【摘要】：目的體外誘導(dǎo)擴(kuò)增獲取大量高純度的小鼠骨髓來源樹突狀細(xì)胞(DC),并研究不同生長狀態(tài)的DC成熟情況及進(jìn)行生物學(xué)特性鑒定。方法以重組小鼠粒細(xì)胞巨噬細(xì)胞集落刺激因子(rmGM-CSF)和重組小鼠白細(xì)胞介素-4(rmIL-4),體外誘導(dǎo)小鼠骨髓細(xì)胞分化為DC,脂多糖(LPS)刺激24h,倒置顯微鏡動態(tài)觀察細(xì)胞形態(tài)學(xué)變化,并分別收集懸浮細(xì)胞、貼壁細(xì)胞,流式細(xì)胞術(shù)(FACS)分析DC細(xì)胞表面分子CD11c、MHCⅡ類分子、CD80和CD86的表達(dá)水平,并應(yīng)用混合淋巴細(xì)胞反應(yīng)(MLR)檢測其刺激異基因淋巴細(xì)胞增殖的能力。結(jié)果經(jīng)體外誘導(dǎo)培養(yǎng)第3天即可見大量細(xì)胞集落形成;培養(yǎng)至第9天,細(xì)胞形態(tài)不規(guī)則,具有典型DC形態(tài)。同組懸浮DC表面MHCⅡ類分子和共刺激分子CD80、CD86的表達(dá)水平高于貼壁DC。LPS刺激24h后,DC表面MHCⅡ類分子、CD80、CD86的表達(dá)水平顯著升高,同時可以顯著刺激同種異基因淋巴細(xì)胞增殖。結(jié)論體外簡易誘導(dǎo)培養(yǎng)可以獲得高純度小鼠骨髓來源的各具特性的DC,為開展DC的相關(guān)實驗研究提供大量穩(wěn)定的細(xì)胞來源。
[Abstract]:Objective to obtain a large number of high purity mouse bone marrow derived dendritic cells (DC) by induction amplification in vitro, and to study the maturation and biological characteristics of DC in different growth states. Methods the colony of murine granulocyte macrophages was obtained by recombinant mouse granulocyte macrophage colony. The stimulating factor rmGM-CSF and recombinant mouse interleukin-4rmIL-4 were induced to differentiate into DCand lipopolysaccharide (LPS) for 24 h. The morphological changes were observed by inverted microscope. Suspension cells, adherent cells and FACS were collected to analyze the expression levels of CD11cMHC class 鈪，

本文編號：1508738