發(fā)布時(shí)間：2018-01-01 08:36

  本文關(guān)鍵詞：IL-17和P38信號(hào)通路在三叉神經(jīng)痛ION-CCI模型大鼠中的研究　出處：《安徽醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 三叉神經(jīng)痛 眶下神經(jīng)慢性縮窄術(shù) 三叉神經(jīng)節(jié) IL-17

【摘要】：目的選用成年雄性SD大鼠,經(jīng)眶下神經(jīng)慢性縮窄術(shù)建立大鼠三叉神經(jīng)痛模型,通過行為學(xué)實(shí)驗(yàn)驗(yàn)證模型是否建立成功、Western blot實(shí)驗(yàn)檢測(cè)三叉神經(jīng)節(jié)內(nèi)IL-17和P-P38蛋白的表達(dá)量、RT-PCR實(shí)驗(yàn)檢測(cè)三叉神經(jīng)節(jié)內(nèi)IL-17A、CXCL1、CXCL2的m RNA表達(dá)量,探討IL-17和P38信號(hào)通路在ION-CCI模型大鼠中作用機(jī)制。方法將雄性SD大鼠隨機(jī)分為兩組,ION-CCI組:成年雄性SD大鼠,經(jīng)顴骨下緣入路,行眶下神經(jīng)慢性縮窄術(shù);sham組:單純暴露眶下神經(jīng)而不結(jié)扎。分別在術(shù)前、術(shù)后1 w、術(shù)后2 w、術(shù)后3 w對(duì)兩組大鼠的觸須墊機(jī)械痛閾進(jìn)行測(cè)定,記錄結(jié)果并比較,確定大鼠TN模型是否建立成功;提取三叉神經(jīng)節(jié)組織總蛋白,采用Western blot實(shí)驗(yàn)方法檢測(cè)三叉神經(jīng)節(jié)內(nèi)IL-17和P-P38的表達(dá)量,根據(jù)實(shí)驗(yàn)結(jié)果條帶的灰度值進(jìn)行半定量分析比較目的蛋白的表達(dá)量變化;提取三叉神經(jīng)節(jié)組織總m RNA,利用RT-PCR實(shí)驗(yàn)檢測(cè)IL-17A、CXCL1和CXCL2的m RNA相對(duì)表達(dá)量;所有實(shí)驗(yàn)數(shù)據(jù)均采用SPSS17.0軟件進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果與sham組相比,ION-CCI組大鼠的機(jī)械痛閾明顯降低,并在2 w時(shí)痛閾達(dá)到最低(sham組:14.11±0.81 g vs ION-CCI組:4.45±0.92 g,n=5,P0.01)。與建模前相比,ION-CCI組大鼠的TG在建模后1 w、2 w、3 w時(shí)IL-17和P-P38的蛋白表達(dá)量均明顯增高,并在2 w時(shí)表達(dá)量達(dá)到峰值,3 w時(shí)有所下降,但仍高于建模前,且差異有統(tǒng)計(jì)學(xué)意義(n=3,P0.05)。在建模后2 w時(shí),與sham組相比,ION-CCI組大鼠的TG中,IL-17及相關(guān)趨化因子(CXCL1、CXCL2)的m RNA表達(dá)量明顯升高,且IL-17A組差異有統(tǒng)計(jì)學(xué)意義(n=3,P0.05)。結(jié)論IL-17與三叉神經(jīng)痛的發(fā)生密切相關(guān);IL-17可能是通過P38信號(hào)通路參與三叉神經(jīng)痛的發(fā)生。
[Abstract]:Objective to select the adult male SD rats with chronic constriction of the infraorbital nerve to establish a rat model of trigeminal neuralgia, through the behavioral experiment model is established successfully, the expression of Western was detected by blot assay and P-P38 protein IL-17 in the trigeminal ganglion, trigeminal ganglion RT-PCR assay of IL-17A, CXCL1, expression of M RNA CXCL2 the role of IL-17 and P38 signaling pathway in ION-CCI rats. Methods male SD rats were randomly divided into two groups, group ION-CCI: adult male SD rats by the zygomatic margin approach for infraorbital nerve chronic constriction; group sham: simple and not exposed infraorbital nerve ligation. Respectively in preoperative, postoperative 1 W, 2 W after operation, 3 W after the operation of two groups of rat vibrissa pad mechanical pain threshold were measured and recorded the results and comparison, determine the TN rat model is established successfully; extraction of trigeminal ganglion tissue total protein by Western blot. The expression assay in the trigeminal ganglion of IL-17 and P-P38, according to the experimental results with the gray value of the semi quantitative analysis of the variation of the expression of target protein is extracted; trigeminal ganglion tissue total m RNA, using RT-PCR IL-17A m RNA CXCL1 assay, and the expression of CXCL2; all of the experimental data by SPSS17.0 the software for statistical analysis. Results compared with sham group, the mechanical pain threshold of rats in ION-CCI group were significantly decreased, and at 2 W reached the lowest threshold (Group sham: 14.11 + 0.81 g vs ION-CCI group: 4.45 + 0.92 g, n=5, P0.01). Compared with before modeling, the rats of group ION-CCI in TG modeling after 1 W, 2 W, 3 W IL-17 and P-P38 protein expression were significantly increased, and in 2 w expression reached the peak at 3 W decreased, but still higher than that before modeling, and the difference was statistically significant (n=3, P0.05). In 2 W after modeling, compared with sham group, ION-C The rats of group CI in TG, IL-17 and related chemokines (CXCL1, CXCL2) m RNA expression was significantly increased, and there was significant difference in group IL-17A (n=3, P0.05). Conclusion IL-17 and trigeminal neuralgia is closely related to the occurrence of; IL-17 may be through P38 pathway in trigeminal neuralgia.

【學(xué)位授予單位】：安徽醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R745.11;R-332

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 韓良;崔曼曼;徐文華;劉悅雁;王烈成;王元銀;;三叉神經(jīng)痛模型大鼠三叉神經(jīng)節(jié)神經(jīng)元中NPR-A表達(dá)量的變化[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2016年08期

2 顏劍豪;李盟;汪天悅;詹文峰;江桂華;;三叉神經(jīng)痛患者腦灰質(zhì)結(jié)構(gòu)的變化:一項(xiàng)基于體素的形態(tài)學(xué)測(cè)量[J];南方醫(yī)科大學(xué)學(xué)報(bào);2015年08期

3 馬騰飛;王霽蕾;黃姍姍;秦健;韓良;趙利;肖芳莉;汪聰;王元銀;;經(jīng)眶下孔注射相關(guān)炎癥因子制造大鼠三叉神經(jīng)痛模型的實(shí)驗(yàn)研究[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2015年06期

4 黃姍姍;徐文華;馬騰飛;肖芳莉;劉衍釗;王烈成;王元銀;;三叉神經(jīng)痛患者部分促炎因子水平變化及意義[J];安徽醫(yī)科大學(xué)學(xué)報(bào);2015年05期

5 孫暉;李國(guó)超;王元銀;王烈成;;眶下神經(jīng)慢性縮窄環(huán)三叉神經(jīng)痛動(dòng)物模型的研究進(jìn)展[J];醫(yī)學(xué)綜述;2013年12期

6 秦泗佳;王福;;原發(fā)性三叉神經(jīng)痛發(fā)病機(jī)制的研究進(jìn)展[J];中華神經(jīng)外科雜志;2012年12期

7 葛永紅;;三叉神經(jīng)痛病因及治療現(xiàn)狀[J];武警醫(yī)學(xué)院學(xué)報(bào);2010年01期

8 王福,張奎啟,裘罡;三叉神經(jīng)痛的病因和發(fā)病機(jī)制學(xué)研究的進(jìn)展[J];大連醫(yī)科大學(xué)學(xué)報(bào);2004年04期

9 張景熙;IL-17的研究進(jìn)展[J];國(guó)外醫(yī)學(xué)(免疫學(xué)分冊(cè));2003年06期

10 徐默菡,牛忠英,倪龍興;谷氨酸樣陽(yáng)性神經(jīng)終末和NMDAR_1、GluR_1在大鼠三叉脊束核尾側(cè)亞核的分布[J];西南國(guó)防醫(yī)藥;2003年01期

相關(guān)博士學(xué)位論文 前1條

1 孫彩霞;SNL大鼠Th17細(xì)胞脊髓浸潤(rùn)及其中樞敏化的可能機(jī)制研究[D];江蘇大學(xué);2011年

，

本文編號(hào)：1363758