TLR2在實驗腦型瘧中的作用研究
[Abstract]:Objective to investigate the role of Toll-like receptor 2 (Toll-like receptors 2, TLR2) in experimental cerebral malaria (experimental cerebral malaria,ECM induced by Plasmodium berghei ANKA strain (Plasmodium berghei ANKA,Pb ANKA. Methods TLR2-/-C57BL/6 mice were infected with Pb ANKA in three ways: mosquito bite infection, megaspore quantitative infection and intrared protozoa infection, and WT C57BL/6 mice were used as control. When mosquito bites, Anopheles stephensi is first infected with Plasmodium Pb ANKA, and then Anopheles stephensi is infected with Anopheles stephensi by biting experimental mice. When the spores were quantitatively infected, Anopheles stephensi was first infected with Plasmodium Pb ANKA, then the spores of Anopheles stephensi were obtained by dissecting the saliva gland of Anopheles, and the experimental mice were infected by quantitative injection. When WT C57BL/6 mice were infected with Plasmodium Pb ANKA, the red blood cells (parasitized?Red?Blood?Cell,pRBC) of Plasmodium falciparum were obtained, and then the mice were quantitatively infected with pRBC. The erythrocyte protozoa rate, survival rate and ECM incidence of experimental mice were observed to determine whether TLR2 was involved in the occurrence of ECM. Results there was no significant difference in protozoa rate between TLR2-/-C57BL/6 mice and WT C57BL/6 mice during ECM infection (6 脳 9 days), indicating that TLR2 deletion had no significant effect on the proliferation of Pb ANKA Plasmodium in mice. The typical CM symptoms such as paralysis and coma began to occur in both groups after 6 days of infection. ECM and death occurred in all mice within 9 days, the incidence of ECM was 100%, and the survival rate of mice was 0. 5%. There was no significant difference in erythrocyte protozoa rate between the two groups, the survival rate of mice was 0 in TLR2-/- group, 100% in WT group and 90% in WT group. There was no significant difference in erythrocyte protozoa rate between the two groups. ECM occurred and died 8 days after infection, the incidence of ECM was 100%, and the survival rate of mice was 0. 5%. Conclusion the deletion of TLR2 in three kinds of infection mice has no effect on the process of ECM, which indicates that the occurrence of ECM does not depend on the participation of TLR2.
【作者單位】： 第三軍醫(yī)大學基礎(chǔ)醫(yī)學部病原生物學教研室;
【基金】：國家自然科學基金項目(No.81301456) 重慶市自然科學基金項目(No.cstc2012jjA10048)
【分類號】：R531.3
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