【摘要】：第一部分日本血吸蟲尾蚴逸出晝夜節(jié)律性及光照周期變化對(duì)其影響 目的:觀察感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出的晝夜節(jié)律性，以及光照周期變化對(duì)此特征的影響。 方法:以安徽（山區(qū)）和江蘇（湖區(qū)）兩地感染性釘螺為研究對(duì)象，分別將釘螺置于自然光照、晝夜顛倒、7:00～9:00插入短暫黑暗、17:00～19:00插入短暫黑暗這四種光照周期條件下連續(xù)逸蚴24h，記錄每2h時(shí)間段內(nèi)感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出數(shù)量，，從而探討光照周期變化與尾蚴逸出的關(guān)系。 結(jié)果：在自然光照周期下，山區(qū)感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出高峰時(shí)段為下午17:00～21:00，表現(xiàn)為‘晚逸蚴’；湖區(qū)感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出高峰時(shí)段為上午7:00～11:00，表現(xiàn)為‘早逸蚴’。在晝夜顛倒的光照周期下，湖區(qū)感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出節(jié)律性出現(xiàn)逆轉(zhuǎn)；上午7:00～9:00插入短暫黑暗會(huì)相應(yīng)延遲山區(qū)和湖區(qū)感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出高峰時(shí)段；下午17:00～19:00插入短暫黑暗對(duì)兩地感染性釘螺體內(nèi)日本血吸蟲尾蚴逸出節(jié)律性幾乎沒有影響。 結(jié)論：日本血吸蟲尾蚴逸出呈現(xiàn)晝夜節(jié)律性，光照周期變化會(huì)相應(yīng)改變尾蚴逸出節(jié)律性。 第二部分日本血吸蟲尾蚴的趨向吸附作用以及溫度和尾蚴時(shí)齡對(duì)其影響 目的：觀察日本血吸蟲尾蚴對(duì)油酸的趨向吸附能力，以及尾蚴時(shí)齡和水溫對(duì)此特征的影響。 方法：利用蜂蠟、指甲油的可凝固性與不同濃度（0.15g/ml、0.3g/ml、0.6g/ml）油酸混合后制備成一種皮膚模擬物，供尾蚴吸附�？瞻捉M、蜂蠟組、指甲油組作為對(duì)照組。模擬小鼠腹部貼片感染尾蚴的方法，用接種環(huán)挑取50±5條尾蚴，置于預(yù)先制備好的皮膚模擬物上，一段時(shí)間后滴加盧戈氏碘液固定染色，顯微鏡下計(jì)算皮膚模擬物上尾蚴斷尾率�；蚪胰ドw玻片，用去氯水多次沖洗皮膚模擬物后滴加盧戈氏碘液固定染色，顯微鏡下計(jì)數(shù)經(jīng)去氯水沖洗后仍吸附在皮膚模擬物上的尾蚴數(shù)量。 結(jié)果：油酸組皮膚模擬物上尾蚴斷尾率及吸附數(shù)量與對(duì)照組相比較存在極其顯著性差異（P＜0.01）。尾蚴與皮膚模擬物接觸時(shí)間相等時(shí)，0.15g/ml油酸組與0.3g/ml油酸組皮膚模擬物上尾蚴斷尾率存在顯著性差異（P＜0.05），0.3g/ml油酸組與0.6g/ml油酸組皮膚模擬物上尾蚴斷尾率不存在顯著性差異。油酸濃度相同時(shí)，逸出后0h尾蚴與逸出后5h尾蚴在皮膚模擬物上吸附數(shù)量無顯著性差異；逸出后5h尾蚴與逸出后10h尾蚴在皮膚模擬物上吸附數(shù)量存在顯著性差異（P＜0.05）。水溫變化（25～35℃）對(duì)尾蚴斷尾率及吸附數(shù)量均無顯著性差異。 結(jié)論：日本血吸蟲尾蚴對(duì)油酸具有趨向吸附作用，尾蚴時(shí)齡影響尾蚴趨向吸附的能力。水溫變化（25～35℃）對(duì)其影響不大。
[Abstract]:The effect of the circadian rhythm of the first part of Schistosoma japonicum and the changes of the photoperiod Objective: To observe the circadian rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis and the shadow of the changes in the photoperiod. In this paper, Oncomelania hupensis (Oncomelania hupensis) in Anhui (mountain area) and Jiangsu (lake area) were used as the research object, and the snail was put into the natural light, the day and night was reversed,7:00 ~ 9:00 was inserted into the short dark, and 17:00 ~ 19:00 was inserted into the short dark. After 24 h, the amount of the tail of Schistosoma japonicum in the infected snails was recorded for every 2 h, so as to study the changes of the illumination period and the escape of the tail. The results showed that during the natural light period, the peak period of the tail of Schistosoma japonicum in the infected snails was 17:00 to 21:00 in the afternoon, and the peak period was 7:00 a.m. to 7:00 AM in the infected Oncomelania hupensis. 11:00, performance as In the period of day and night, the occurrence of the rhythm of the tail of Schistosoma japonicum in the infected Oncomelania hupensis in the lake area is reversed; the insertion of the short darkness at 7:00 to 9:00 in the morning will delay the peak period of the tail of the Schistosoma japonicum in the infected snails in the mountain area and the lake area; and 17: 00-19:00 insertion of short-time dark on the rhythm of the tail of Schistosoma japonicum in the infected snails in the two places There is no effect. Conclusion: The circadian rhythm of the tail of Schistosoma japonicum and the change of light cycle can be changed accordingly. The rhythm of the tail of the tail, the tendency of the second part of the tail of the Schistosoma japonicum, and the temperature. Objective: To observe the tendency of the tail of Schistosoma japonicum to the tendency of oleic acid to adsorb and the tail of the tail. The effect of age and water temperature on this characteristic was studied. The method was prepared by mixing the setting of beeswax and nail polish with different concentration (0.15 g/ ml, 0.3 g/ ml, 0.6 g/ ml) of oleic acid. The invention relates to a skin mimetic, which is used for the absorption of the tail gas. The blank group Cera Flava and nail polish group were used as control group. The method for simulating the infection of the tail of the abdominal patch of mice was carried out by using the inoculation ring to pick up 50% of the tail of the tail of the skin, and then put it on the pre-prepared skin dummy. After a period of time, the iodine solution of Lugol's iodine was added for fixation, and the microscope was under the microscope. The tail-tail rate of the skin-simulating object is calculated, or the cover glass is removed, and after the skin simulation is washed with the dechlorine water for several times, the lugohlet iodine solution is added for fixing and dyeing, the under-microscope counting is still absorbed after the chlorine-removing water is washed, The number of tails attached to the skin mimetics. Results: The tail and tail and the number of adsorption on the skin of the oleic acid group were compared with the control group. There was a significant difference (P <0.01). When the contact time of the tail hair and the skin analog was the same, there was a significant difference (P <0.05), 0.3 g/ ml of oleic acid group and 0.6 g/ ml of the skin of the oleic acid group. There was no significant difference in the tail-tail rate of the tail of the skin. When the concentration of the oleic acid was the same, there was no significant difference in the number of adsorption of the tail of the tail of the tail of the tail of the tail after the escape and the 5-hour after the escape. There was a significant difference (P <0.05). The change of water temperature (25 ~ 35 鈩

本文編號(hào)：2499309