【摘要】：目的研究廣西艾滋病病毒1型流行株的亞型及env基因C2V3區(qū)序列變異特征。方法 2009-2012年共收集138份廣西HIV-1感染者血漿樣本,提取病毒RNA,用巢式聚合酶鏈反應(yīng)對(duì)env基因C2V3區(qū)序列進(jìn)行擴(kuò)增,并測(cè)序,用MEGA、Hy Phy等軟件對(duì)序列信息進(jìn)行分析。結(jié)果 138份樣本中,CRF01_AE亞型72例;CRF08_BC亞型40例;B亞型13例;CRF07_BC亞型8例;C亞型2例。各亞型基因離散率分別為:CRF01_AE(0.052±0.053);CRF08_BC(0.044±0.048);CRF07_BC(0.030±0.041);B亞型(0.024±0.044)。各亞型之間基因離散率差異無(wú)統(tǒng)計(jì)學(xué)意義(F=1.260,P=0.291)。CRF01_AE的負(fù)向選擇壓力比CRF08_BC大(t=10.060,P0.001)。共發(fā)現(xiàn)7種V3環(huán)頂端四肽,其中CRF01_AE有6種,CRF08_BC亞型有2種,兩者均以GPGQ為主;B(B’)亞型有2種,以GPGR為主,CRF07_BC僅出現(xiàn)了1種。在CRF01_AE亞型和B亞型中分別發(fā)現(xiàn)了新的V3環(huán)頂端四肽GPGM和GTGR。各亞型輔助受體均主要為CCR5。結(jié)論 CRF01_AE亞型在廣西HIV-1流行株中占優(yōu)勢(shì),其V3環(huán)頂端四肽以GPGQ為主。CRF01_AE亞型和B亞型V3環(huán)序列出現(xiàn)了新的變異特征。廣西HIV-1流行株主要為巨噬細(xì)胞嗜性的非合胞體誘導(dǎo)型。
[Abstract]:Objective to study the subtypes of HIV 1 epidemic strain and the sequence variation of C2V3 region of env gene in Guangxi. Methods A total of 138 plasma samples of HIV-1 infected people in Guangxi were collected from 2009 to 2012. The C2V3 region of env gene was amplified by nest polymerase chain reaction (NPCR) and sequenced. The sequence information was analyzed by MEGA,Hy Phy and other software. Results among the 138 samples, there were 72 cases of CRF01_AE subtypes, 40 cases of CRF08_BC subtypes, 13 cases of subtypes B, 8 cases of CRF07_BC subtypes and 2 cases of subtypes C. The gene dispersion rates of each subgroup were CRF01_AE (0.052 鹵0.053), CRF08_BC (0.044 鹵0.048) and CRF07_BC (0.030 鹵0.041); B) (0.024 鹵0.044), respectively. There was no significant difference in gene dispersion rate among subtypes (F 鈮，

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