發(fā)布時間：2018-11-22 20:11

【摘要】：目的研究不同濃度的亞砷酸鈉(NaAsO2)對體外培養(yǎng)的細(xì)粒棘球蚴原頭節(jié)生長及相關(guān)抗氧化酶活性的影響,探討NaAsO2可能的作用機(jī)制。方法將不同濃度的NaAsO2(3、6、9、12和15μmol/L)作用于體外培養(yǎng)的細(xì)粒棘球蚴原頭節(jié),經(jīng)0.1%伊紅染色后于光鏡下觀察原頭節(jié)的活力,實驗獨立重復(fù)3次。不同濃度NaAsO2作用原頭節(jié)24h后,采用半胱氨酸天冬氨酸蛋白酶-3(caspase-3)檢測試劑盒檢測caspase-3酶活性;采用化學(xué)比色法或ELISA法測定NaAsO2作用后原頭節(jié)抗氧化酶活性的變化。結(jié)果 12μmol/L和15μmol/L NaAsO2對原頭節(jié)殺傷作用明顯,從作用第1d開始,原頭節(jié)活力開始下降,培養(yǎng)至第10d時12μmol/L組原頭節(jié)存活率僅為12.64%,15μmol/L組原頭節(jié)全部死亡。不同濃度(≥6μmol/L)NaAsO2作用原頭節(jié)24h后caspase-3表達(dá)較正常對照組顯著增高(P0.05)。NaAsO2作用后的原頭節(jié)抗氧化酶(SOD、GST、HO-1和NQO-1)活性均顯著下降(P0.05),且呈時間及劑量依賴性。結(jié)論亞砷酸鈉能顯著抑制細(xì)粒棘球蚴原頭節(jié)生長,并顯著降低抗氧化酶的活性,推測這種抑制作用與下調(diào)抗氧化酶活力有關(guān),然而關(guān)于亞砷酸鈉的具體作用機(jī)制有待于進(jìn)一步研究。
[Abstract]:Objective to study the effects of different concentrations of sodium arsenite (NaAsO2) on the growth of proganglion and the activities of antioxidant enzymes in cultured echinococcus granulosus in vitro, and to explore the possible mechanism of NaAsO2. Methods different concentrations of NaAsO2 (3Cephalococcus granulosus 12 and 15 渭 mol/L) were applied to cultured echinococcus granulosus in vitro. After 0.1% eosin staining, the activity of the protocephalic ganglion was observed under light microscope, and the experiment was repeated three times independently. The activity of caspase-3 was detected by cysteine aspartate protease 3 (caspase-3) assay kit 24 hours after treatment with different concentrations of NaAsO2, and the changes of antioxidant enzyme activity of NaAsO2 were measured by chemical colorimetry or ELISA method. Results the killing effect of 12 渭 mol/L and 15 渭 mol/L NaAsO2 on the protocephalic ganglia was obvious. From the 1st day, the activity of the protocephalic ganglia began to decrease, and the survival rate of the 12 渭 mol/L group was only 12.64% and the survival rate of the 15 渭 mol/L group was 12.64% on the 10th day. At different concentrations (鈮，

本文編號：2350439