【摘要】：目的：分析青海地區(qū)藏族人群中TNF-α基因的2個(gè)位點(diǎn)-238及-308與慢性乙型肝炎易感性之間的關(guān)系，并篩選出相關(guān)的主要位點(diǎn)，為青海地區(qū)藏族人群的HBV易感性風(fēng)險(xiǎn)的預(yù)測(cè)奠定分子學(xué)基礎(chǔ)。從而為臨床診斷及預(yù)防慢性乙型肝炎提供新的思路和方法。 方法： (1)研究對(duì)象：以250例青海地區(qū)藏族慢性乙肝患者(病例組)及134例健康者(對(duì)照組)做為研究對(duì)象。 (2)基因多態(tài)性分析：采用血液基因組小量提取試劑盒提取外周血DNA，運(yùn)用飛行質(zhì)譜技術(shù)分析測(cè)定基因位點(diǎn)TNF-α的-238A/G、-238A/A、-238G/G、-308G/A、-308G/G的基因型。 (3)數(shù)據(jù)分析：計(jì)算各位點(diǎn)基因型及等位基因頻率，比較組間各基因型的差異，，并進(jìn)行Hardy-Weinberg平衡吻合度檢驗(yàn)，應(yīng)用logistic多元回歸分析各基因型與乙肝易感的相關(guān)性。 結(jié)果： (1)-238位點(diǎn)在病例組以及對(duì)照組之間的頻率分布:-238A/G在病例組和對(duì)照組分別為8.4%和8.2%，經(jīng)統(tǒng)計(jì)學(xué)分析結(jié)果表明,各組間的分布頻率未見(jiàn)顯著性差異；-238A/A在病例組和對(duì)照組分別為0.4%和0.0%，經(jīng)統(tǒng)計(jì)學(xué)分析結(jié)果表明,各組間的分布頻率未見(jiàn)顯著性差異；-238G/G在病例組和對(duì)照組分別為91.2%和91.8%，經(jīng)統(tǒng)計(jì)學(xué)分析結(jié)果表明,各組間的分布頻率未見(jiàn)顯著性差異； (2)-308在各臨床類型中的分布：-308G/A在慢性病毒性乙型肝炎以及正常對(duì)照組分別為7.6%和7.5%,各組間的分布頻率比較亦未見(jiàn)顯著性統(tǒng)計(jì)學(xué)差異；-308G/G在各組分別為92.4%和92.5%,各組間的分布頻率比較亦未見(jiàn)顯著性統(tǒng)計(jì)學(xué)差異； 結(jié)論： (1)TNF-α-238基因位點(diǎn)多態(tài)性與青海地區(qū)藏族人群HBV感染的易感性可能無(wú)關(guān)； (2)TNF-α-308基因位點(diǎn)多態(tài)性與青海地區(qū)藏族人群HBV感染的易感性可能無(wú)關(guān)。
[Abstract]:Objective: to analyze the relationship between the susceptibility of chronic hepatitis B (CHB) and the two loci of TNF- 偽 gene-238 and -308 in Tibetan population in Qinghai area, and to screen out the main related loci. To lay a molecular foundation for the prediction of HBV susceptibility risk in Qinghai Tibetan population. So as to provide new ideas and methods for clinical diagnosis and prevention of chronic hepatitis B. Methods: (1) study subjects: 250 Tibetan chronic hepatitis B patients (case group) and 134 healthy persons (control group) in Qinghai. (2) Polymorphism Analysis of Gene: peripheral DNA, was extracted from peripheral blood by using a small quantity extraction kit of blood genome. The TNF- 偽 gene locus was analyzed by FMS to determine -238A / G / -238A / An / 238G / A / G / 308G / A, -genotype of 308G/G. (3) data analysis: the frequencies of genotypes and alleles were calculated, the differences of genotypes were compared, and the Hardy-Weinberg equilibrium coincidence test was performed. The correlation between genotypes and susceptibility to hepatitis B was analyzed by logistic multivariate regression analysis. Results: (1) the frequency distribution of -238 locus in the case group and the control group:-238A/G was 8.4% and 8.2% in the case group and the control group, respectively. There was no significant difference in distribution frequency among groups. -238A/A in the case group and the control group were 0.4% and 0.0.The results of statistical analysis showed that there was no significant difference in the distribution frequency among the groups. -238G/G was 91.2% and 91.8% in the case group and the control group, respectively. The results of statistical analysis showed that there was no significant difference in the distribution frequency among the three groups. (2) the distribution of -308 in each clinical type:-308G/A in chronic toxic hepatitis B and normal control group were 7.6% and 7.5%, respectively. -308G/G was 92.4% and 92.5% in each group, and there was no significant statistical difference in the distribution frequency between each group. Conclusion: (1) the polymorphism of TNF- 偽 -238 gene may not be associated with the susceptibility to HBV infection in Tibetan population in Qinghai; (2) the polymorphism of TNF- 偽 -308 gene may not be associated with the susceptibility to HBV infection in Tibetan population in Qinghai.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R512.62

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