【摘要】：目的探討和比較熒光RT-PCR檢測(cè)與ELISA檢測(cè)在戊肝臨床診斷的效果。方法從我院健康體檢中心2013年1月至2016年1月的50500血清標(biāo)本中隨機(jī)選擇100份作為研究標(biāo)本。通過(guò)采用酶聯(lián)免疫法對(duì)戊肝抗體的檢測(cè),然后再通過(guò)熒光RT-PCR檢測(cè)法進(jìn)行確診,觀察和比較兩組檢測(cè)方法的檢查結(jié)果。結(jié)果陽(yáng)性一致率為55%,陰性一致率為100%,總一致率為75%,Kappa值為0.59(0.4Kappa≤0.75),表示兩者一致性一般。ELISA陽(yáng)性檢出率為30%,PCR陽(yáng)性檢出率為50%,PCR陽(yáng)性檢出率明顯高于ELISA,數(shù)據(jù)比較差異具有統(tǒng)計(jì)學(xué)意義,P=0.0000.005。結(jié)論熒光RTPCR檢測(cè)和ELISA檢測(cè)在戊肝的診斷中都具有一定的檢查準(zhǔn)確度,同時(shí)熒光RT-PCR檢測(cè)的陽(yáng)性檢出率明顯高于ELISA檢測(cè)。由于ELISA檢測(cè)容易受到各方面因素的影響而發(fā)生漏診的情況,因此建議對(duì)于ELISA檢測(cè)出現(xiàn)可疑的標(biāo)本,使用RT-PCR進(jìn)行確診,使更具可靠性。
[Abstract]:Objective to investigate and compare the clinical diagnosis of hepatitis E by fluorescence RT-PCR and ELISA. Methods from January 2013 to January 2016, 50500 serum samples were randomly selected. The antibody against hepatitis E was detected by enzyme-linked immunosorbent assay (Elisa), and then confirmed by fluorescence RT-PCR assay. The results of the two methods were observed and compared. Results the positive consistency rate was 55, the negative consistency rate was 100 and the total consistency rate was 75. 59 (0.4Kappa 鈮，

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