【摘要】：目的：探討Toll樣受體4基因在HIV相關型KS (Kaposi's sarcoma)腫瘤組織和及正常組織中的表達。方法：收集2011年5月至2013年7月在新疆醫(yī)科大學第一附屬醫(yī)院及新疆傳染病醫(yī)院就診并經病理明確診斷的患者KS腫瘤組織35例,包括HIV-KS26例,經典KS9例,應用實時熒光定量PCR技術、免疫組化方法檢測TLR4在卡波西肉瘤組織中的表達與分布。應用Western Blot方法檢測TLR4信號通路上蛋白的表達。結果：TLR4基因在KS腫瘤組織中mRNA水平的表達均低于正常組織,差異有統(tǒng)計學意義(P=0.0017),經典KS及正常對照之間表達有顯著性差異(P=0.0497), HIV-KS與正常對照之間表達也有顯著性差異(P=0.0054)。而在經典KS及HIV-KS之間表達無統(tǒng)計學意義(P=0.46)。免疫組化中,在經典KS及HIV-KS病損皮膚組織真皮層可見TLR4陽性細胞,主要表達于內皮細胞和異形細胞胞漿和胞膜。蛋白水平：HIV-KS及經典KS組織中可見到Ras、Raf、ERK、IκB-α、P50、P65表達較正常組織高,P0.05,且有顯著性差異,而在]HIV-KS和經典KS之間無顯著性差異。其余蛋白在正常及KS之間表達無顯著性差異。結論：在艾滋病患者中,TLR4低表達與HIV-KS的發(fā)生密切相關。
[Abstract]:Aim: to investigate the expression of Toll-like receptor 4 gene in HIV-associated KS (Kaposi's sarcoma) tumors and normal tissues. Methods: from May 2011 to July 2013, 35 patients with KS tumor tissue, including HIV-KS26, classic KS9, were collected from the first affiliated Hospital of Xinjiang Medical University and Xinjiang Infectious Diseases Hospital. The expression and distribution of TLR4 in Kaposi's sarcoma were detected by real-time fluorescence quantitative PCR and immunohistochemistry. Western Blot method was used to detect the expression of protein in TLR4 signaling pathway. Results: the expression of TLR4 gene in KS was significantly lower than that in normal tissue (P0. 0017). There was a significant difference between classical KS and normal control (P0. 0497). There was also a significant difference between HIV-KS and normal control (P0. 0054). However, there was no significant difference between the expression of KS and HIV-KS (P < 0. 46). In immunohistochemistry, TLR4 positive cells were found in the dermis of classical KS and HIV-KS lesions, mainly expressed in the cytoplasm and membrane of endothelial cells and allogeneic cells. Protein level: the expression of Ras,Raf,ERK,I 魏 B- 偽 and P50 p65 in HIV-KS and classical KS tissues was higher than that in normal tissues, and there was significant difference between HIV-KS and classical KS, but there was no significant difference between HIV-KS and classical KS. There was no significant difference in the expression of other proteins between normal and KS. Conclusion: the low expression of TLR4 is closely related to the occurrence of HIV-KS in AIDS patients.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R512.91

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