【摘要】：目的觀察氯硝柳胺原位固化長效注射劑對感染日本血吸蟲(Schistosoma japonicum)的小鼠和水牛的療效。方法 50只昆明小鼠隨機均分為2組,給藥組小鼠皮下注射低濃度氯硝柳胺原位固化長效注射劑(71 mg/ml)450 mg/kg 1次,對照組小鼠不作任何處理。給藥后第1、15、43、57和71天,各組分別取5只小鼠采用腹部貼片法感染日本血吸蟲尾蚴(80±4)條/鼠。35 d后,處死小鼠,檢獲成蟲,計算減蟲率。10頭水牛分成2組,給藥組4頭、對照組6頭。給藥組水牛皮下注射高濃度氯硝柳胺原位固化長效注射劑(222 mg/ml)30 mg/kg1次,對照組水牛不作任何處理。于給藥后第1和3個月糞檢,計算糞檢陽性率,并觀察水牛的不良反應。采用SPSS19.0軟件進行統(tǒng)計學分析,組間比較采用t檢驗。結果給藥后第1、15、43、57和71天感染日本血吸蟲尾蚴,35 d后給藥組小鼠的蟲荷分別為(3.6±1.4)條、(17.5±8.6)條、(10.0±6.8)條、0條、(11.3±8.4)條,均少于對照組小鼠的蟲荷(P0.05),且減蟲率均超過50.0%。第1和3個月對水牛進行糞檢,給藥組均為陰性,對照組分別有2和3頭陽性,其中1頭牛給藥后8 h出現(xiàn)精神沉郁。結論氯硝柳胺原位固化長效注射劑預防小鼠感染日本血吸蟲尾蚴的作用達2個月,預防水牛感染日本血吸蟲尾蚴的作用達3個月,具有現(xiàn)場應用預防血吸蟲感染的潛力。
[Abstract]:Objective to observe the effect of niclosamide in situ solidified long-acting injection on mice and buffalo infected with Schistosoma japonicum (Schistosoma japonicum). Methods Fifty Kunming mice were randomly divided into two groups. The mice in the control group were given low concentration of niclosamide in situ solidified long-term injection (71 mg/ml) for 450 mg/kg subcutaneously, and the control group was not treated with any treatment. 5 mice were infected with cercariae of Schistosoma japonicum (80 鹵4) per mouse by abdominal patch method in each group on the 1st day after administration. After 35 days, the mice were killed, the adult worms were seized, and the rate of worm reduction was calculated. 10 buffalo were divided into 2 groups and 4 in the administration group. There were 6 heads in the control group. High concentration of niclosamide in situ solidified long-acting injection (222 mg/ml) was injected subcutaneously for 30 mg/kg1 in the treatment group, and no treatment was given in the control group. The fecal positive rate was calculated at 1 and 3 months after administration, and the adverse reactions of buffalo were observed. SPSS19.0 software was used for statistical analysis and t test was used for comparison between groups. Results the Schistosoma japonicum cercariae were infected with Schistosoma japonicum cercariae on day 1, 15, 43, 57 and 71, respectively. After 35 days of administration, the worm load of mice in the administration group was (3.6 鹵1.4), (17.5 鹵8.6), (10.0 鹵6.8), 0, (11.3 鹵8.4), respectively, which was lower than that in the control group (P0.05), and the worm reduction rate was more than 50.0%. In the first and third months, the faecal examination of buffalo was negative in the administration group, and 2 and 3 positive in the control group. One of the buffaloes developed depression at 8 hours after administration. Conclusion niclosamide in situ solidified long-term injection can prevent mice from infection with Schistosoma japonicum cercariae for 2 months and buffalo for 3 months. It has the potential to prevent Schistosoma japonicum infection in the field.
【作者單位】： 中國疾病預防控制中心寄生蟲病預防控制所世界衛(wèi)生組織熱帶病合作中心科技部國家級熱帶病國際聯(lián)合研究中心衛(wèi)生部寄生蟲病原與媒介生物學重點實驗室;上海健康醫(yī)學院;
【基金】：上海市公共衛(wèi)生三年行動計劃(No.GWIV-29,15GWZK0101) 上海市衛(wèi)生和計劃生育委員會科研課題(No.20154Y0051) 國家重點研發(fā)計劃“重要熱帶病相關入侵媒介生物及其病原的動態(tài)分布與資源庫建設(No.2016YFC1202000)”~~
【分類號】：R532.21
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本文編號：2268967