【摘要】：目的觀察轉(zhuǎn)染pc DNA3.1-HBV Creg DNA質(zhì)粒的小鼠肝細胞表面干性分子CD44、CD90、CD133的表達變化。方法從含有pc DNA3.1-HBV Creg DNA質(zhì)粒的Ecoli DH5α菌株中提取pc DNA3.1-HBV Creg DNA質(zhì)粒。取SPF級正常小鼠的肝細胞,常規(guī)培養(yǎng),待細胞貼壁后,加入50μL pc DNA3.1-HBV Creg DNA混合轉(zhuǎn)染細胞。采用免疫細胞化學染色法檢測轉(zhuǎn)染前后小鼠肝細胞表面干性分子CD44、CD90、CD133表達。結(jié)果轉(zhuǎn)染前,部分小鼠肝細胞CD90、CD133呈陽性,且陽性染色細胞都是圓形。未見有CD44陽性染色細胞。轉(zhuǎn)染pc DNA3.1-HBV Creg DNA后,小鼠肝細胞表面干性分子CD44、CD90、CD133呈陽性染色。結(jié)論轉(zhuǎn)染pc DNA3.1-HBV Creg DNA質(zhì)粒的小鼠肝細胞表達CD44、CD90、CD133。HBV Creg DNA可能影響小鼠肝細胞表面干性分子CD44的表達。
[Abstract]:Objective to observe the expression of dry molecule CD44,CD90,CD133 on the surface of mouse hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid. Methods pc DNA3.1-HBV Creg DNA plasmid was extracted from Ecoli DH5 偽 strain containing pc DNA3.1-HBV Creg DNA plasmid. The hepatocytes of SPF grade normal mice were cultured routinely. After the cells were adhered, 50 渭 L pc DNA3.1-HBV Creg DNA was added into the transfected cells. The expression of dry molecule CD44,CD90,CD133 on hepatocytes of mice before and after transfection was detected by immunocytochemical staining. Results before transfection, some mouse hepatocytes were positive for CD90,CD133, and the positive staining cells were round. No CD44 positive staining cells were found. After transfection of pc DNA3.1-HBV Creg DNA, CD44,CD90,CD133 positive staining was observed on the surface of mouse hepatocytes. Conclusion the expression of CD44,CD90,CD133.HBV Creg DNA in murine hepatocytes transfected with pc DNA3.1-HBV Creg DNA plasmid may affect the expression of dry molecule CD44 on the surface of mouse hepatocytes.
【作者單位】： 黔西南民族職業(yè)技術學院醫(yī)藥系;
【基金】：貴州省科技廳科學技術基金資助項目(2013-2268)
【分類號】：R735.7;R512.62
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本文編號：2226907