本文選題：肺炎克雷伯菌 + 噬菌體治療��； 參考：《中國病原生物學(xué)雜志》2015年05期

【摘要】：目的觀察肺炎克雷伯菌噬菌體LH-01治療不同宿主菌所致小鼠敗血癥的效果,分析影響療效因素,探索評(píng)價(jià)噬菌體療效的方法。方法通過比較噬菌斑和成斑率,從9株LH-01宿主菌中選擇對(duì)其敏感性較強(qiáng)的菌株。尾靜脈注射絕對(duì)致死量的不同敏感菌致小鼠敗血癥,2h后通過尾靜脈注射LH-01治療并觀察其療效。比較LH-01在小鼠新鮮血清、補(bǔ)體熱滅活血清,以及在標(biāo)準(zhǔn)補(bǔ)體溶血活性由低至高的溶液中對(duì)敏感菌的殺滅能力,明確引起LH-01對(duì)不同敏感菌感染組小鼠療效差異的因素。結(jié)果 9株宿主菌中KP6、KP13、KP16和KP22在營養(yǎng)肉湯中對(duì)LH-01最為敏感。當(dāng)小鼠全身感染致死量的這4種細(xì)菌后,LH-01的治療可使KP6感染組小鼠生存率達(dá)90%(9/10),KP13組為30%(3/10),而KP16組和KP22組小鼠全部死亡。體外試驗(yàn)顯示,除LH-01對(duì)KP6的作用不受補(bǔ)體影響外,LH-01對(duì)其余3株菌的殺滅作用均受補(bǔ)體影響,其中25U/ml的補(bǔ)體活性即可完全抑制LH-01對(duì)KP16和KP22的殺滅作用,而完全抑制對(duì)KP13的殺滅作用所需的補(bǔ)體活性≥50U/ml。結(jié)論補(bǔ)體是影響LH-01治療小鼠肺炎克雷伯菌感染療效的最主要原因。血清中噬菌體對(duì)致病菌的殺菌力和補(bǔ)體干擾殺菌的程度有助于預(yù)測(cè)噬菌體療效,指導(dǎo)細(xì)菌感染的噬菌體治療。
[Abstract]:Objective to observe the efficacy of Klebsiella pneumoniae bacteriophage LH-01 in the treatment of septicemia in mice induced by different host bacteria, analyze the influencing factors and explore the method of evaluating the efficacy of bacteriophage. Methods by comparing plaque and plaque forming rate, 9 strains of LH-01 were selected. Mice with septicemia were treated by tail vein injection of LH-01 2 hours after injection of different sensitive bacteria with absolute lethal dose and the curative effect was observed. To compare the killing ability of LH-01 against sensitive bacteria in fresh serum, heat-inactivated serum and standard complement hemolytic activity from low to high solution, the factors that caused the difference of the efficacy of LH-01 to mice infected with different sensitive bacteria were determined. Results KP6, KP13, KP16 and KP22 were most sensitive to LH-01 in nutrient broth. The survival rate of mice in KP6 infected group was 90% (9 / 10), and that in KP13 group was 30% (3 / 10), while that in KP16 and KP22 groups were all dead. In vitro experiments showed that the killing effect of LH-01 on KP6 was not affected by complement, and the cytotoxicity of LH-01 to KP16 and KP22 could be completely inhibited by the complement activity of 25 U / ml, and the effect of LH-01 on KP16 and KP22 was completely inhibited by the addition of LH-01 to KP16 and KP22, and the effect of LH-01 on KP16 and KP22 was inhibited by LH-01. The complement activity required to completely inhibit the killing effect of KP13 was more than 50 U / ml. Conclusion complement is the most important factor affecting the efficacy of LH-01 in the treatment of Klebsiella pneumoniae infection in mice. The bactericidal ability of bacteriophages in serum and the degree of complement interference in bactericidal efficacy can be used to predict the efficacy of bacteriophage and to guide the bacteriophage therapy of bacterial infection.
【作者單位】： 河南醫(yī)學(xué)高等�？茖W(xué)校病原生物學(xué)與免疫學(xué)教研室;鄭州大學(xué)基礎(chǔ)醫(yī)學(xué)院病原生物學(xué)教研室;
【基金】：河南省科技廳重點(diǎn)科技攻關(guān)項(xiàng)目(No.142102310440) 河南省醫(yī)學(xué)科技攻關(guān)計(jì)劃項(xiàng)目(No.201303199)
【分類號(hào)】：R515.3

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