發(fā)布時(shí)間：2018-06-12 21:31

  本文選題：青海地區(qū) + 藏族��； 參考：《青海大學(xué)》2013年碩士論文

【摘要】：目的：探討青海地區(qū)藏族人群白介素-10（IL-10）基因多態(tài)性與乙型肝炎病毒(HBV)易感的相關(guān)性。本文主要研究IL-10-592、-1082位點(diǎn)基因單核苷酸多態(tài)性(SNP)與乙型病毒性肝炎易感性的關(guān)系。 方法：采集青海地區(qū)藏族人群血樣，根據(jù)乙肝五項(xiàng)及血清標(biāo)記物檢測(cè)結(jié)果抽取HBV感染患者血樣250例作為病例組，抽取健康者外周血134例作為對(duì)照組。通過(guò)離心機(jī)離心獲得單個(gè)核細(xì)胞及血清，由單個(gè)核細(xì)胞中提取基因組DNA，選取細(xì)胞因子IL-10-592、-1082位點(diǎn)進(jìn)行多態(tài)性分析。最終通過(guò)統(tǒng)計(jì)學(xué)分析該基因在感染者與健康者之間有無(wú)顯著差異。 結(jié)果：1.在病例組中IL-10-592C/C、C/A、A/A位點(diǎn)各基因型頻率分別為24.0%、52.0%、24.0%，與對(duì)照組IL-10-592C/C、C/A、A/A位點(diǎn)各基因型頻率分別為23.9%、46.33%、29.9%比較，無(wú)顯著差異，不具有統(tǒng)計(jì)學(xué)意義。在logistic多元回歸分析中發(fā)現(xiàn)，與IL-10-592A/A基因型比較，攜帶IL-10-592C/A、C/C基因型的群體發(fā)生乙型病毒性肝炎的危險(xiǎn)性分別為OR=1.398（95%CI=0.847-2.308），p=0.198，OR=1.250（95%CI=0.695-2.247），p=0.551，均無(wú)統(tǒng)計(jì)學(xué)意義。2.在病例組中IL-10-1082G/G、G/A、A/A位點(diǎn)各基因型頻率分別為1.2%、23.2%、75.6%，與對(duì)照組IL-10-1082G/G、G/A、A/A位點(diǎn)各基因型頻率分別為4.5%、19.4%、76.1%比較，無(wú)顯著差異，不具備統(tǒng)計(jì)學(xué)意義。在logistic多元回歸分析中發(fā)現(xiàn)，與IL-10-1082A/A比較，攜帶IL-10-1082G/A、G/G基因型的群體發(fā)生乙型病毒性肝炎的危險(xiǎn)性分別為OR=1.204（95%CI=0.715-2.028），p=0.516，OR=1.270（95%CI=0.760-2.102），p=0.075，，均無(wú)統(tǒng)計(jì)學(xué)意義。 結(jié)論：1.IL-10-592各基因型頻率無(wú)顯著差異。攜帶IL-10-592C/A、C/C基因型的群體與青海地區(qū)藏族人群乙型病毒性肝炎易感不相關(guān)。2.IL-10-1082各基因型頻率無(wú)顯著差異。攜帶IL-10-1082G/A、G/G基因型的群體與青海地區(qū)藏族人群乙型病毒性肝炎易感不相關(guān)。
[Abstract]:Objective: To investigate the correlation between the polymorphism of interleukin -10 (IL-10) gene and the susceptibility to hepatitis B virus (HBV) in the Tibetan population in Qinghai. This paper mainly studies the relationship between the single nucleotide polymorphisms (SNP) of the IL-10-592 and -1082 loci and the susceptibility of hepatitis B virus.
Methods: collecting blood samples from Tibetan population in Qinghai area, 250 cases of HBV infected patients were selected according to the five items of hepatitis B and the detection results of serum markers. 134 cases of peripheral blood from healthy people were selected as the control group. The single nucleus cells and serum were obtained by centrifuge centrifuge. The genomic DNA was extracted from single nuclear cells, and the cytokine was selected. The polymorphisms were analyzed by IL-10-592 and -1082 loci.
Results: 1. the genotype frequencies of IL-10-592C/C, C/A and A/A loci in the case group were 24%, 52%, 24% respectively. The genotype frequencies of IL-10-592C/C, C/A and A/A in the control group were 23.9%, 46.33%, 29.9%, respectively, and no significant difference was found. In logistic multivariate regression analysis, it was found to be compared with the IL-10-592A/A genotype, The risk of viral hepatitis B with IL-10-592C/A and C/C genotypes was OR=1.398 (95%CI=0.847-2.308), p=0.198, OR=1.250 (95%CI=0.695-2.247), p=0.551, and.2. was not statistically significant in the case group, IL-10-1082G/G, G/A, and the frequencies of the genotypes were 1.2%, 23.2%, and 75.6% respectively. The genotype frequencies of the A/A loci were 4.5%, 19.4%, and 76.1%, respectively, and no significant difference was found. In logistic multivariate regression analysis, the risk of viral hepatitis B with IL-10-1082G/A and G/G genotype was OR=1.204 (95%CI=0.715-2.028), p=0.516, OR=1.270 (95), respectively. %CI=0.760-2.102), p=0.075, no statistical significance.
Conclusion: there is no significant difference in the genotype frequencies of 1.IL-10-592. There is no significant difference in the genotype frequencies of IL-10-592C/A, C/C genotype and the susceptibility to viral hepatitis B in Qinghai Tibetan population. The population with IL-10-1082G/A, G/G genotype and the susceptibility to hepatitis B in the Tibetan people of Qinghai Haiti region are susceptible to viral hepatitis It's not related.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2013
【分類號(hào)】：R512.62

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本文編號(hào)：2011150