本文選題：流感病毒 + HN亞型��； 參考：《中國(guó)病原生物學(xué)雜志》2017年07期

【摘要】：目的了解2010-2016年唐山市分離的H3N2亞型流感病毒株血凝素HA基因特征和變異規(guī)律。方法隨機(jī)選取2010-2016年唐山市分離的20株H3N2亞流感毒株提取RNA,采用RT-PCR擴(kuò)增目的片段,測(cè)序,采用MEGA 6.0軟件構(gòu)建HA基因核苷酸系統(tǒng)進(jìn)化樹(shù),采用BioEdit v7.2.5軟件比較分析其核苷酸序列特征及氨基酸變異情況。結(jié)果 0株病毒HA1區(qū)核苷酸和氨基酸同源性分別為92.4%和89.1%。系統(tǒng)進(jìn)化和序列分析顯示,2010-2011流行年度分離的5株病毒分別屬于HA基因1和5分支,其中1分支毒株在抗原決定簇發(fā)生E50K,I260M,R261Q氨基酸替換,5分支毒株發(fā)生D53N、Y94H、I230V和E280A替換;2011-2016流行年度分離的15株H3N2亞型病毒屬于3C分支,與疫苗株A/Perth/16/2009比較抗原表位發(fā)生S45N、T48I、A198S、N278K和N312S位點(diǎn)突變,并逐年進(jìn)化為3C.1(2010-2011)、3C-2012/13(2012-2013)、3C.3(2013-2014)、3C.3a(2014-2015)和3C.2a(2015-2016)分支。與相應(yīng)疫苗株相比,3C.3a和3C.2a分支毒株抗原表位A、B及受體結(jié)合部位均發(fā)生了氨基酸位點(diǎn)突變,其中3C.2a毒株擁有Q311H(C區(qū))和N171K(D區(qū))位點(diǎn)突變。結(jié)論 2010-2016年唐山市分離株A/H3N2亞型流感病毒發(fā)生抗原漂移,未來(lái)應(yīng)關(guān)注3C.2a分支流行株的變化。
[Abstract]:Objective to investigate the characteristics and variation of hemagglutinin HA gene of influenza virus strain H3N2 isolated from Tangshan City from 2010 to 2016. Methods Twenty strains of H3N2 subinfluenza virus isolated from Tangshan City from 2010 to 2016 were randomly selected to extract RNAs. The target fragments were amplified by RT-PCR and sequenced. The phylogenetic tree of HA gene was constructed by MEGA6.0 software. The nucleotide sequence and amino acid variation of BioEdit v7.2.5 were compared and analyzed. Results the homology of nucleotide and amino acid in HA1 region of 0 strains were 92.4% and 89.1%, respectively. Phylogeny and sequence analysis showed that the five viruses isolated in 2010-2011 belong to the HA gene 1 and 5 branches, respectively. Among them, 1 branch of H3N2 subtype virus belonged to 3C branch in the antigen determinant cluster E50KN I260MU R261Q amino acid replacement strain 5. The 15 H3N2 subtype viruses isolated in the epidemic year 2011-2016 belonged to the 3C branch. Compared with the vaccine strain A / P / 16 / 2009, the antigen epitopes of S45NN T48IU A198S278K and N312S were mutated. And it has evolved into 3C-2012 / 3C-2012 / 3C.3 / 3C.3 / 3C.3a / 3C.3a (2013-2014 / 2015) and 3C.2aer 2015-2016 (respectively), and has evolved into three branches: 3C.1a (2010-2011) and 3C.2a (2015-2016). In comparison with the corresponding vaccine strains, amino acid site mutations were found in the epitopes of 3C.3a and 3C.2a, and in the receptor binding sites, among which the 3C.2a strains had Q311HnC) and N171KGF-D) mutations. Conclusion Antigen drift of influenza virus A / H _ 3N _ 2 subtype occurred in Tangshan City from 2010 to 2016, so we should pay attention to the change of 3C.2a branch epidemic strain in the future.
【作者單位】： 唐山市疾病預(yù)防控制中心微生物科;
【基金】：河北省醫(yī)學(xué)研究重點(diǎn)課題(No.ZL20171385)
【分類(lèi)號(hào)】：R440;R511.7

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