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  本文選題：泡球蚴 + 肝星狀細(xì)胞�。� 參考：《青海大學(xué)》2014年碩士論文

【摘要】：目的：研究泡球蚴囊液刺激環(huán)境對大鼠肝星狀細(xì)胞（HSC-T6）TGF-β1、IL-6、TNF-α表達(dá)的影響。 方法：用濃度分別為0、0.01、0.025、0.05、0.1、0.2、0.4、0.9、1.7、3.4、6.8、13.5mg/ml的泡球蚴囊液與大鼠HSC-T6細(xì)胞共培養(yǎng)24h，0mg/ml濃度組做空白對照。ELISA法(酶聯(lián)免疫吸附測定法)檢測各濃度泡球蚴囊液處理組轉(zhuǎn)化生長因子β1（TGF-β1）、白細(xì)胞介素6（IL-6）、腫瘤壞死因子-α（TNF-α）的表達(dá)變化。 結(jié)果：TGF-β1的表達(dá)在泡球蚴囊液濃度為0.01～6.8mg/ml范圍內(nèi)略高于空白對照組，但差異無統(tǒng)計(jì)學(xué)意義；泡球蚴囊液濃度高于3.4mg/ml時，IL-6的表達(dá)極顯著增加（P 0.01），其中6.8、13.5mg/ml泡球蚴囊液處理組相比空白對照組分別增加3028.65、3879.23倍；泡球蚴囊液濃度≤0.2mg/ml時，TNF-α的表達(dá)趨于平穩(wěn)；此后急劇增加又逐漸下降，，其中泡球蚴囊液濃度為0.4mg/ml時TNF-α的表達(dá)量達(dá)到最大，為空白對照組的3.53倍，差異具統(tǒng)計(jì)學(xué)意義（P0.05）。 結(jié)論：泡球蚴囊液對大鼠HSC-T6細(xì)胞TGFβ1無明顯的刺激分泌作用；高濃度的泡球蚴囊液可刺激大鼠HSC-T6細(xì)胞IL-6的表達(dá)分泌；一定濃度的泡球蚴囊液可刺激HSC-T6細(xì)胞TNF-α分泌。
[Abstract]:Objective: To study the effect of echinococcus cyst fluid stimulation on the expression of TGF- beta 1, IL-6 and TNF- alpha in rat hepatic stellate cells (HSC-T6).
Methods: the alveolar cyst fluid with the concentration of 0,0.01,0.025,0.05,0.1,0.2,0.4,0.9,1.7,3.4,6.8,13.5mg/ml and the rat HSC-T6 cells were co cultured with 24h, and the concentration group of 0mg/ml was used as the blank control.ELISA (enzyme linked immunosorbent assay) to detect the transforming growth factor beta 1 (TGF- beta 1), interleukin 6 (IL-6), and the swelling of interleukin 6 (IL-6) in the treatment group. The changes in the expression of tumor necrosis factor - alpha (TNF- - alpha).
Results: the expression of TGF- beta 1 was slightly higher than that of the blank control group in the range of 0.01 ~ 6.8mg/ml of alveolar cyst fluid, but the difference was not statistically significant. The expression of IL-6 was significantly increased (P 0.01) when the concentration of vesicle cyst fluid was higher than that of 3.4mg/ml. The 6.8,13.5mg/ml alveolar cyst fluid was increased by 3028.653879.23, respectively, compared with the blank control group. When the concentration of vesicle cyst fluid was less than 0.2mg/ml, the expression of TNF- alpha tended to be stable and then increased rapidly and gradually, and the expression of TNF- a was the maximum when the concentration of vesicle cyst fluid was 0.4mg/ml, which was 3.53 times that of the blank control group, and the difference was statistically significant (P0.05).
Conclusion: the hydatid cyst fluid has no obvious stimulating effect on TGF beta 1 of rat HSC-T6 cells, and the high concentration of alveolar cyst fluid can stimulate the expression and secretion of IL-6 in rat HSC-T6 cells, and a certain concentration of vesicle sac can stimulate the secretion of TNF- alpha in HSC-T6 cells.
【學(xué)位授予單位】：青海大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R532.32

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 孟存仁;張瓊;張朝霞;;Em18抗原ELISA法對泡球蚴病診斷價值的系統(tǒng)評價[J];中國循證醫(yī)學(xué)雜志;2009年07期

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