本文選題：發(fā)熱伴血小板減少綜合征新布尼亞病毒 + 酶聯(lián)免疫吸附實驗　； 參考：《鄭州大學》2017年碩士論文

【摘要】：發(fā)熱伴血小板減少綜合征(Severe Fever with Thrombocytopenia Syndrome,SFTS)是近年來新發(fā)現(xiàn)的一種傳染病,新布尼亞病毒(New Bunyavirus)是該病的病原體[1-2]。河南省是我國乃至全世界首次發(fā)現(xiàn)SFTS的地區(qū),也是報告病例數(shù)最多的地區(qū)[1,3],目前尚無我省SFTS流行地區(qū)自然人群中新布尼亞病毒的抗體水平及隱性感染狀況報告。信陽市作為我省SFTS發(fā)病數(shù)最多的地區(qū)[4],是SFTS理想的研究現(xiàn)場。目的:通過調查我省SFTS流行地區(qū)新布尼亞病毒的抗體水平及隱性感染狀況,了解人群中新布尼亞病毒的自然感染率和人群免疫狀況,為我省SFTS疫情防控提供依據(jù)。方法:本次調查采用分層隨機抽樣法,最終在信陽市的平橋區(qū)和新縣各選取7個自然村作為調查點;于2016年4月~5月在調查點采集調查對象的血清標本;采用酶聯(lián)免疫吸附法(enzyme-linked immunosorbent assay,ELISA)對標本進行新布尼亞病毒特異性IgG和IgM抗體檢測;根據(jù)采集標本信息對檢測結果進行分類整理,應用統(tǒng)計學分析方法,研究新布尼亞病毒特異性抗體在不同性別、不同年齡組、不同地區(qū)人群中的分布情況。對IgM抗體陽性的血清標本,采用熒光定量PCR(Real-time PCR)進行新布尼亞病毒核酸檢測,同時采用細胞培養(yǎng)技術進行病毒分離并鑒定;于標本采集1個月后,對所有IgM抗體陽性者逐一進行隨訪,確證其是否為隱性感染者。結果:本次橫斷面調查在信陽地區(qū)共抽取調查對象1463人,平橋區(qū)695人,新縣768人;男性409人,女性1054人;年齡分布從2歲到95歲,年齡中位數(shù)為60歲。1463名被調查者中,新布尼亞病毒IgG抗體陽性者有153人,總體陽性率為10.46%(153/1463),平橋區(qū)和新縣分別為7.19%(50/695)和13.41%(103/768),差異有統(tǒng)計學意義(P0.05);不同性別和不同年齡組間IgG抗體陽性率差異無統(tǒng)計學意義(P0.05)。新布尼亞病毒IgM抗體陽性者有12人,總體陽性率為0.82%(12/1463),平橋區(qū)和新縣分別為0.43%(3/695)和1.17%(9/768),差異無統(tǒng)計學意義(P0.05);不同性別和不同年齡組間IgM抗體陽性率差異也無統(tǒng)計學意義(P0.05)。12份IgM抗體陽性血清標本中,新布尼亞病毒核酸陽性標本有6份;經細胞培養(yǎng),成功從5份血清標本中分離出新布尼亞病毒,病毒分離率為41.67%(5/12),其中平橋1份,新縣4份。距標本采集1個月后隨訪,IgM抗體陽性者均無SFTS臨床癥狀出現(xiàn),確系隱性感染者。結論:信陽地區(qū)自然人群中新布尼亞病毒Ig G抗體水平較高,人群中新布尼亞病毒感染的流行強度較強,新布尼亞病毒感染譜中存在隱性感染或病毒攜帶者,人群有較高的新布尼亞病毒潛在感染風險。
[Abstract]:Fever with thrombocytopenia syndrome (Severe Fever with Thrombocytopenia Syndromeg SFTS) is a newly discovered infectious disease in recent years, and New Bunyavirusis is the pathogen of the disease [1-2]. Henan Province is the region where SFTS was first discovered in China and the whole world, and also the region with the largest number of reported cases. At present, there is no report on the antibody level and recessive infection of Neobunia virus in the natural population of SFTS epidemic area in Henan Province. Xinyang is the region with the highest incidence of SFTS in our province [4], which is an ideal site for SFTS research. Objective: to investigate the level of antibody and recessive infection of Neobunia virus in epidemic area of SFTS in our province, to understand the natural infection rate and immune status of new Bunia virus in population, and to provide the basis for the prevention and control of SFTS epidemic situation in our province. Methods: stratified random sampling method was used to select 7 natural villages in Pingqiao District and Xinxian County of Xinyang City, and serum samples were collected from April to May 2016. Enzyme-linked immunosorbent assaysa was used to detect the specific IgG and IgM antibodies of new Bunia virus, and the results were classified and sorted according to the information collected from the samples, and the statistical analysis method was used. To study the distribution of specific antibodies against NBV in different gender, age groups and different regions. The new Bunia virus nucleic acid was detected by fluorescence quantitative PCR(Real-time PCR, and the virus was isolated and identified by cell culture technique. All patients with positive IgM antibody were followed up one by one to confirm whether they were recessive infection. Results: a total of 1463 subjects were selected from this cross-sectional survey, including 695 in Pingqiao District, 768 in Xinxian County, 409 in males and 1054 in females. The age distribution ranged from 2 to 95 years old, with a median age of 60 years. The positive rate of IgG antibody in Pingqiao district and Xinxian county was 7.1950 / 695 and 13.41 / 103 / 768, respectively. The difference was statistically significant (P 0.05), but there was no significant difference in the positive rate of IgG antibody between different sex and different age groups (P 0.05). There are 12 people who are positive for IgM antibodies to the New Bunia virus. The overall positive rate was 0.82% 1463%, 0.43% in Pingqiao District and 0.43% in Xinxian County, respectively. There was no significant difference in the positive rate of IgM antibody between the two groups (P 0.05). There was no significant difference in the positive rate of IgM antibody among different sex and age groups. New Bunia virus was isolated from 5 serum samples by cell culture. The isolation rate of the virus was 41.675 / 12, of which 1 was Pinqiao and 4 were Xinxian. One month after the collection of specimens, no clinical symptoms of SFTS were found in all the patients who were positive for IgM antibody, and they were indeed recessive infected. Conclusion: the level of IgG antibody to neobonia virus in natural population in Xinyang area is high, and the epidemic intensity of new Bunia virus infection in population is stronger. There is recessive infection or virus carrier in infection spectrum of new Bunia virus. The population has a higher risk of potential new Bunia virus infection.
【學位授予單位】：鄭州大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R512.8;R181.3

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